FastQCFastQC Report
Fri 17 Jun 2016
SRR1526479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21053
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1050.4987412720277395No Hit
CATCCCGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.35149384885764495No Hit
CATCCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT600.2849950125872797No Hit
CATCCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC560.2659953450814611No Hit
CATCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAG540.25649551132855175No Hit
CATCCCGGGATGGGGAGAAGGTGGTGACAGAGTCCCAGAAGGAACAGCACAGTGAC490.23274592694627846No Hit
CATCCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA480.22799601006982376No Hit
CATCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC470.2232460931933691No Hit
CATCCCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC450.21374625944045977No Hit
CATCCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA430.20424642568755047No Hit
CATCCCGGGATGTCCGAGCTGGAGAAGGCCATGGTTGCCCTCATTGATGTCTTCCA400.1899966750581865No Hit
CATCCCGGGACATCGACACAGCAGCCAAGTTCATTGGTGCTGGGGCCGCCACAGTT370.17574692442882248No Hit
CATCCCGGGATGAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.17099700755236782No Hit
CATCCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA350.16624709067591317No Hit
CATCCCGGGGGATTCTCTCTGCGTTTGCTGTCGCTGCAGCAGCGGACGACCTCCTT340.16149717379945852No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC290.1377475894171852No Hit
CATCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT280.13299767254073055No Hit
CATCCCGGGAGGGCTTGTTTTCTTAGACTAATAGGGGCAGTAAAGTAGACGGTGGC240.11399800503491188No Hit
CATCCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC240.11399800503491188No Hit
CATCCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.10449817128200256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGG251.8730683E-550.6280236
CACAGGG251.8730683E-550.6280237
GGTTGCC150.0080677350.38461333
TCCCAGA150.0080677350.38461333
AACCACC150.0080677350.38461331
GTCCCAG150.0080677350.38461332
AGTCCCA150.0080677350.38461331
AGGGGAT251.9553123E-550.2637940
ATGCTGA251.9553123E-550.2637940
CAGCACA150.00830099750.0238745
ATAGGCC150.00830099750.0238745
GAACAGC150.00830099750.0238742
AAAACAT252.0117865E-550.0238747
CAGTGAC150.00830099750.0238750
GATAGGC150.00830099750.0238744
AACATCG204.080195E-450.0238749
CGGGGGT355.0389644E-849.9047666
CGGGATC700.049.9047666
CGGGAGA650.049.9047666
CGGGACT355.0389644E-849.9047666
Produced by FastQC (version 0.11.5)