FastQCFastQC Report
Fri 17 Jun 2016
SRR1526478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151170
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7130.4716544287887809No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.45577826288284706No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5710.377720447178673No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.35258318449427795No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.27055632731362045No Hit
ACCTGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3610.23880399550175302No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3570.236157967850764No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3420.22623536415955547No Hit
ACCTGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3300.2182972812065886No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2830.18720645630746843No Hit
ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2830.18720645630746843No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2630.17397631805252364No Hit
ACCTGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2550.16868426275054574No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2450.16206919362307337No Hit
ACCTGAGGGGTTACATGGGACCACGAAATGCAATCTCTCTTTCTGAATCTGAATGTCTGCTCAT2310.15280809684461202No Hit
ACCTGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2230.14751604154263412No Hit
ACCTGAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1980.13097836872395316No Hit
ACCTGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1920.12700932724746974No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.1230402857709863No Hit
ACCTGAGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTTC1760.11642521664351393No Hit
ACCTGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1760.11642521664351393No Hit
ACCTGAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA1750.11576370973076669No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1640.10848713369054706No Hit
ACCTGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC1610.10650261295230534No Hit
ACCTGAGGGAGTGTTTGTGAATCAATTAATTGTGATTGCACAAAAATAATTTTAAAAATGTGGT1580.10451809221406363No Hit
ACCTGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1570.1038565853013164No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1560.10319507838856916No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1530.10121055765032745No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1520.1005490507375802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTATT201.9756018E-458.1719952
CCCTATC201.9788546E-458.15264548
CTATCGG201.9788546E-458.15264550
AATTGTT201.9788546E-458.15264546
CCTATCG201.9788546E-458.15264549
TATCGGG201.9788546E-458.15264551
TCTATTC150.004630889358.15264545
TAAGCAT351.5950718E-858.1526445
GTTAGAA201.9821117E-458.13331240
CTGACTA150.004636992258.13331242
GACTACT201.9821117E-458.13331244
CGTGCAA201.9886385E-458.0946937
TAGTTAG201.9886385E-458.0946938
CCATAGA201.9886385E-458.0946937
TACCCGC303.7318387E-758.01758632
CCTCGAA150.00467373558.01758630
CGGATTC150.00467373558.01758634
GACGGAT150.00467373558.01758632
CCTTGAT150.00467373558.01758632
TCGAAGA150.00467373558.01758632
Produced by FastQC (version 0.11.5)