FastQCFastQC Report
Fri 17 Jun 2016
SRR1526477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147238
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6020.40886184273081677No Hit
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.39663673779866615No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5650.38373246037028486No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.2506146511090887No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3540.2404270636656298No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.21461850880886726No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2650.17998071150110706No Hit
ACCTGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2540.17250981404257054No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2530.17183064154633992No Hit
ACCTGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2520.17115146905010936No Hit
ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2280.15485132914057512No Hit
ACCTGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2210.150097121666961No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2110.14330539670465506No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2110.14330539670465506No Hit
ACCTGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2070.14058870671973267No Hit
ACCTGAGGGGTTACATGGGACCACGAAATGCAATCTCTCTTTCTGAATCTGAATGTCTGCTCAT2050.1392303617272715No Hit
ACCTGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1660.11274263437427838No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1580.10730925440443363No Hit
ACCTGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1510.10255504693081949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG150.00459079558.27964434
GTAAAGT150.00459079558.27964433
CACAAAG201.9774272E-458.16005357
TCACTAC201.9841261E-458.12029654
GATCAGC150.004647135358.1004553
AACGCGC150.004653427358.080631
CAATCTC453.092282E-1158.080631
TAGTAAG150.004653427358.080631
TTTAGGA150.004659725458.06077648
AAACTAA150.004659725458.06077648
GTTTAGG150.004659725458.06077647
TAAACTA150.004659725458.06077647
ACTAACT150.004659725458.06077650
CATACCG150.004659725458.06077646
ATCATCT150.004659725458.06077647
TACGCGC201.9975784E-458.0409645
TAATTGT201.9975784E-458.0409627
AGCGATG201.9975784E-458.0409630
TTACGCG201.9975784E-458.0409644
GCAATCT453.092282E-1158.0409630
Produced by FastQC (version 0.11.5)