##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526477_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147238 Sequences flagged as poor quality 0 Sequence length 64 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.201496896181695 39.0 38.0 40.0 35.0 40.0 2 37.93150545375514 39.0 38.0 40.0 35.0 40.0 3 38.2388649669243 39.0 38.0 40.0 35.0 40.0 4 38.021482226055774 39.0 38.0 40.0 35.0 40.0 5 37.9127738763091 39.0 38.0 40.0 35.0 40.0 6 37.54056018147489 39.0 38.0 40.0 33.0 40.0 7 38.31655550876812 39.0 38.0 40.0 36.0 40.0 8 38.17927437210503 39.0 38.0 40.0 35.0 40.0 9 38.3846493432402 39.0 38.0 40.0 36.0 40.0 10 37.24761950040071 39.0 37.0 40.0 32.0 40.0 11 37.138198019533 39.0 36.0 40.0 32.0 40.0 12 37.258031214767925 39.0 37.0 40.0 32.0 40.0 13 37.28980969586656 39.0 37.0 40.0 32.0 40.0 14 37.28461402627039 39.0 37.0 40.0 33.0 40.0 15 37.11922873171328 39.0 37.0 40.0 33.0 40.0 16 36.95074641057336 39.0 36.0 40.0 32.0 40.0 17 36.60201170893384 39.0 36.0 40.0 31.0 40.0 18 36.865258968472816 39.0 36.0 40.0 32.0 40.0 19 36.819170322878605 39.0 36.0 40.0 31.0 40.0 20 36.66471291378584 38.0 36.0 40.0 31.0 40.0 21 36.71319903829175 39.0 36.0 40.0 31.0 40.0 22 36.594955106698 38.0 36.0 40.0 31.0 40.0 23 36.40721824528994 38.0 35.0 40.0 31.0 40.0 24 36.295725288308724 38.0 35.0 40.0 30.0 40.0 25 36.18216764693897 38.0 35.0 40.0 30.0 40.0 26 36.29136500088293 39.0 36.0 40.0 30.0 40.0 27 35.749575517189854 39.0 35.0 40.0 28.0 40.0 28 35.65940857659028 39.0 35.0 40.0 29.0 40.0 29 35.532321819095614 38.0 35.0 40.0 28.0 40.0 30 35.49759572936335 38.0 35.0 40.0 28.0 40.0 31 35.06307474972493 38.0 35.0 40.0 27.0 40.0 32 35.20462108966435 38.0 35.0 40.0 27.0 40.0 33 35.08096415327565 38.0 34.0 40.0 27.0 40.0 34 34.46795664162784 38.0 33.0 40.0 24.0 40.0 35 34.54916529700213 38.0 33.0 40.0 25.0 40.0 36 33.89718686752061 38.0 33.0 39.0 22.0 40.0 37 33.59458835355004 38.0 33.0 39.0 21.0 40.0 38 33.554238715548976 38.0 33.0 39.0 22.0 40.0 39 33.105434738314834 38.0 32.0 39.0 17.0 40.0 40 33.18764177725859 38.0 32.0 39.0 19.0 40.0 41 32.59767858840788 37.0 32.0 39.0 13.0 40.0 42 32.290251157989104 37.0 31.0 39.0 9.0 40.0 43 32.19302082342873 36.0 31.0 39.0 10.0 40.0 44 32.030141675382716 36.0 31.0 39.0 8.0 40.0 45 31.82655292791263 36.0 31.0 39.0 4.0 40.0 46 31.387501867724364 36.0 30.0 39.0 4.0 40.0 47 31.163218734294137 36.0 30.0 39.0 4.0 40.0 48 30.970041701191267 36.0 29.0 39.0 4.0 40.0 49 30.76507423355384 35.0 29.0 39.0 4.0 40.0 50 30.337249894728263 35.0 29.0 39.0 4.0 40.0 51 29.88368491829555 35.0 28.0 39.0 4.0 40.0 52 29.665724880805225 35.0 28.0 39.0 4.0 40.0 53 29.40559502302395 35.0 27.0 39.0 4.0 40.0 54 29.12051915945612 35.0 26.0 39.0 4.0 40.0 55 28.736582947336963 35.0 25.0 38.0 4.0 40.0 56 28.086648827069098 35.0 22.0 38.0 4.0 40.0 57 27.651278881810402 34.0 21.0 38.0 4.0 40.0 58 27.374672299270568 33.0 20.0 38.0 4.0 40.0 59 27.057002947608634 33.0 18.0 38.0 4.0 39.0 60 26.735312894769013 33.0 14.0 38.0 4.0 39.0 61 26.403374128961275 33.0 4.0 38.0 4.0 39.0 62 26.125694453877397 33.0 4.0 37.0 4.0 39.0 63 25.830804547739035 33.0 4.0 37.0 4.0 39.0 64 25.702603947350546 33.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.06906338523961608 2 2 0.131252147638655 2 3 -0.026118113372483265 2 4 0.45992278980897794 2 5 -0.01559219477999818 2 6 0.16996691348676762 2 7 -0.1810948092881972 2 8 0.4084383901737638 2 9 -0.127324634806115 2 10 0.4637927926444121 2 11 -0.25367153520393515 2 12 -0.27132268838569473 2 13 -0.3949017595239823 2 14 -0.11494657374784367 2 15 0.07756264429665549 2 16 -0.6892886500379731 2 17 -0.018569519216207198 2 18 -0.7512003754731111 2 19 -0.6967846356509142 2 20 -0.15499866594480238 2 21 -0.3339380592445451 2 22 -0.9095452885520956 2 23 -0.2525179070042256 2 24 -0.38010047330324426 2 25 -0.289982621552376 2 26 0.22013264086378825 2 27 0.7565062282296253 2 28 0.5996414247147044 2 29 0.5863922332873699 2 30 0.6009203550976849 2 31 0.623352418713452 2 32 0.1843223169192072 2 33 0.03689412162677996 2 34 0.08760480491832823 2 35 0.34060653231745164 2 36 -0.7378300966167473 2 37 -0.9937833588506919 2 38 -1.030651126447026 2 39 -4.9361902402430715 2 40 -1.1698829399600292 2 41 -1.3658672551733133 2 42 -1.1602189786617494 2 43 -1.3441854467068204 2 44 -0.917816976458294 2 45 -1.323492727981943 2 46 0.10861831684392342 2 47 0.5068646234878678 2 48 0.1143573037666279 2 49 0.1478121884042949 2 50 0.35568395261550734 2 51 0.5634534938097815 2 52 -0.13187410644552244 2 53 0.002946586685308006 2 54 -0.5918950554139712 2 55 -0.6702773024683957 2 56 -0.9529284153770874 2 57 -0.6396701084842888 2 58 -0.8060931311056265 2 59 -1.1587121641586755 2 60 -0.9480074428598293 2 61 -2.268749902147423 2 62 -1.9650689151623766 2 63 -2.8029047225398998 2 64 -2.8313631826041643 3 1 -0.175531209354979 3 2 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1.27205844704595 4 59 1.0496605442140314 4 60 0.7936292987769136 4 61 1.4718945884970687 4 62 1.5105500604565982 4 63 1.3048253851902096 4 64 0.8870265357855516 5 1 -0.040023962978168015 5 2 0.30144407870015755 5 3 -0.18220898685466125 5 4 0.20972694178703932 5 5 -0.0013932131897078648 5 6 0.31195672938962815 5 7 -0.08258325425490654 5 8 0.25029111246126945 5 9 0.09545249014492185 5 10 0.46457618473214524 5 11 -2.8322065919998636 5 12 -2.5174057279470006 5 13 -2.2101191508283335 5 14 -1.9293805729644546 5 15 -1.336362150062925 5 16 -19.26253581024165 5 17 -2.8794194996314104 5 18 -2.850201550561245 5 19 -3.235366695972104 5 20 -2.6643014469867126 5 21 -1.9921832609680123 5 22 -1.9591927621126075 5 23 -2.0007552748068065 5 24 -2.362865847372966 5 25 -2.890158884772113 5 26 0.13023839879563326 5 27 0.6651431259969627 5 28 0.8090029601631983 5 29 0.7897804040668461 5 30 0.057050398184244955 5 31 0.4220206521642922 5 32 0.1580786819799158 5 33 -0.25824884742922904 5 34 0.4658852592857343 5 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-0.02700675722597623 119 44 -0.6455050962327427 119 45 -0.2106694730723575 119 46 -0.08878592192722223 119 47 -0.49080210799181145 119 48 -0.5337566375127807 119 49 -0.2334827756244806 119 50 0.13348873581597331 119 51 -1.4514697027680938 119 52 -1.1720743786601844 119 53 -1.7569211947652086 119 54 -1.8385409819160152 119 55 -0.8256827078738027 119 56 -1.0324539841112923 119 57 -1.0301037075509818 119 58 -1.3045376187587507 119 59 -0.391552918582164 119 60 -1.2696096205771141 119 61 -1.3864341331410053 119 62 -1.2684910423255111 119 63 -1.178949832397958 119 64 -1.3888199199170188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 165.0 5 20.0 6 38.0 7 54.0 8 147.0 9 139.0 10 198.0 11 237.0 12 316.0 13 463.0 14 566.0 15 747.0 16 835.0 17 889.0 18 989.0 19 1217.0 20 1414.0 21 1589.0 22 2059.0 23 2196.0 24 2240.0 25 2465.0 26 2341.0 27 2731.0 28 3167.0 29 3667.0 30 4248.0 31 4997.0 32 6004.0 33 7587.0 34 9280.0 35 11316.0 36 14765.0 37 18583.0 38 21822.0 39 17701.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.28618970646164 0.5637131718713919 0.07674649207405697 0.073350629592904 8 99.1763562598963 0.4634694973190804 0.1889215839511794 0.1712526588334432 9 98.42703649872995 0.9087327999565329 0.2974775533489996 0.36675314796452 10 59.38005134544071 24.68656189298958 5.803528980290414 10.12985778127929 11 35.077221912821415 26.397397410994444 18.406932992841522 20.118447683342616 12 37.24921555576685 16.473328896073024 24.67162009807251 21.605835450087614 13 26.55021122264633 17.08594248767302 29.598337385729227 26.765508903951424 14 24.50318531900732 19.543188579035302 31.439574023010366 24.51405207894701 15 27.002188778770204 20.30806041573202 28.539092132631836 24.15065867286594 16 30.10732966510427 20.180694246314786 24.609061884382854 25.102914204198086 17 31.535374149659862 19.84965986394558 20.367346938775512 28.24761904761905 18 30.631358752495956 22.32575829609204 22.695228134041486 24.347654817370515 19 30.920686235890194 20.554476425922655 21.525693095532404 26.99914424265475 20 27.596983490726274 24.163326312928866 21.741286772199196 26.49840342414566 21 28.952444341813933 20.418641926676536 26.306388296499545 24.322525435009982 22 28.555807604015264 19.867833032233527 25.97495211833902 25.60140724541219 23 30.98384927803964 20.89474184653418 24.65871582064413 23.46269305478205 24 29.862394044772877 24.002934144751144 22.029857639643556 24.104814170832427 25 29.005491070215022 20.726751298012886 23.87460244107973 26.39315519069236 26 28.29417079350846 18.112589754563302 25.30314455154986 28.290094900378378 27 25.026202240461703 18.134672710196416 27.830182258701186 29.008942790640695 28 26.06713437081091 21.1855007178873 25.400962173123116 27.346402738178675 29 27.880325371347737 21.39534251047391 25.906605364818542 24.81772675335981 30 27.504253726264206 19.727761519090723 28.90560130674471 23.86238344790036 31 27.259608184825673 19.44440662736716 30.448041604835748 22.847943582971425 32 30.033200892614165 18.667337941544655 30.272002394818482 21.027458771022697 33 29.458256181243875 19.115564753294848 30.171686090839778 21.2544929746215 34 24.794273052667464 20.751320947673427 35.30731208454065 19.14709391511846 35 24.40539013080396 25.100605334295693 32.24681842013877 18.247186114761575 36 26.728921000613454 22.768727421443664 30.19426078658578 20.3080907913571 37 25.733935886687252 24.372737868842403 30.043828258661705 19.849497985808643 38 26.993463606807666 22.865789240510644 31.269041760668497 18.871705392013197 39 26.227300076578054 20.423777486051854 30.600453998468442 22.74846843890165 40 25.999481851154925 20.280073086258145 31.575717908860344 22.14472715372658 41 21.740969114918695 21.445623704027064 32.66874931790898 24.14465786314526 42 21.797462823121354 19.87204872253554 34.28124701287706 24.049241441466044 43 22.09447603962559 19.08842023335677 36.558089997337355 22.259013729680277 44 24.12771852246129 19.000143304604173 34.64900608020963 22.223132092724903 45 22.909669315420114 19.895688266295295 32.4096829687884 24.784959449496192 46 21.962594486817938 20.946541116702743 32.837370005940635 24.253494390538684 47 22.238381024559104 20.823689259403398 35.12149826235978 21.81643145367772 48 23.364964500273075 21.860322228290553 34.242900054614964 20.53181321682141 49 24.864805811984485 20.679931173867917 33.01196263724259 21.443300376905007 50 21.268916868309887 21.190993725136366 33.5896594621936 23.950429944360142 51 20.998811426697817 23.57883519816386 33.34836127163682 22.07399210350151 52 21.314432109308285 23.33253629376601 33.50777113578138 21.84526046114432 53 23.705429746757595 21.44633803009389 32.96729578658212 21.8809364365664 54 23.46438185818336 21.055220760054112 32.05974391560421 23.420653466158324 55 23.816586246018836 21.760050302773486 31.068113782686552 23.355249668521125 56 22.763066154456098 22.657798679371677 29.664237767782687 24.91489739838954 57 23.785827073065697 24.78245712371747 29.653503585270656 21.778212217946177 58 22.606721286810465 26.345556756165283 29.578996320663105 21.46872563636115 59 25.334281747361654 24.92801400734565 28.87715530507698 20.86054894021572 60 25.851852865509876 23.716404838797963 28.356779243527285 22.07496305216487 61 23.943344247950947 23.552332059581506 28.058542454216713 24.445781238250834 62 23.00870433302108 22.957421931090128 28.415920792620803 25.61795294326799 63 22.255726495726496 24.175042735042734 27.69094017094017 25.878290598290597 64 23.336709415162257 22.608570685572722 28.20990104430423 25.84481885496079 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 3.0 5 3.5 6 1.0 7 2.0 8 3.0 9 14.5 10 47.5 11 69.0 12 98.0 13 162.5 14 198.0 15 417.0 16 932.0 17 1228.0 18 1159.0 19 1090.0 20 1272.5 21 1699.0 22 1943.0 23 2353.0 24 2904.5 25 3046.0 26 3158.0 27 3315.0 28 3360.0 29 2931.0 30 2536.5 31 2571.0 32 2224.5 33 1878.0 34 1955.5 35 1985.0 36 1937.0 37 1910.0 38 1881.5 39 1880.0 40 2052.0 41 2148.0 42 2072.0 43 2185.0 44 2298.0 45 2522.0 46 2733.5 47 2721.0 48 2790.5 49 3185.5 50 3511.0 51 3658.5 52 3978.0 53 4150.0 54 5228.5 55 6787.5 56 7268.0 57 7490.0 58 7712.0 59 8656.5 60 10018.5 61 10436.0 62 10676.0 63 9755.5 64 8595.0 65 7822.5 66 6301.5 67 5553.0 68 4693.5 69 3834.0 70 3429.0 71 2613.0 72 2202.0 73 1949.0 74 1401.0 75 1106.0 76 891.0 77 557.5 78 439.0 79 338.5 80 177.5 81 117.0 82 100.5 83 84.0 84 64.5 85 36.0 86 27.0 87 18.0 88 8.0 89 7.0 90 3.5 91 0.0 92 0.0 93 1.0 94 2.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.05908800717206156 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08421738953259349 16 0.019016829894456595 17 0.16164305410288105 18 0.0 19 0.0 20 0.03260027981906845 21 0.0 22 0.0 23 0.0 24 0.004075034977383556 25 0.061125524660753335 26 0.02105434738314837 27 0.20646843885410016 28 0.18948912644833535 29 0.13990953422350208 30 0.20918512883902254 31 0.22548526874855676 32 0.17250981404257054 33 0.2322769937108627 34 0.3823741153778237 35 0.2560480310789334 36 0.35860307800975294 37 0.3592822505059835 38 0.3538488705361388 39 0.6669473912984419 40 0.38169494288159306 41 0.4278786726252734 42 0.5270378570749399 43 0.5209253046088645 44 0.4733832298727231 45 0.5120960621578668 46 0.5358670995259376 47 0.5263586845787093 48 0.5148127521427892 49 0.5311128920523235 50 0.6384221464567571 51 0.5732215868186202 52 0.5861258642470015 53 0.608538556622611 54 0.5976717966829215 55 0.6275553865170677 56 0.6411388364416795 57 0.6418180089379101 58 0.6900392561702822 59 0.6988684986212799 60 0.7375813309064236 61 0.6458930439152936 62 0.6717015987720562 63 0.6710224262758255 64 0.755239815808419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 147238.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.040234178676705 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40696657661537 41.32357136065418 2 8.210328491445127 8.545348347573317 3 3.834357829894418 5.986226381776444 4 2.332197904023596 4.854725003056276 5 1.4603970087310598 3.799970116410166 6 1.029716925727262 3.215202597155625 7 0.7595630554794253 2.7669487496434346 8 0.5155110084439398 2.146185088088673 9 0.41632408023700457 1.9499042366780315 >10 1.887161818253005 16.357869571713824 >50 0.09396656356446498 3.4196335185210343 >100 0.04959346410346763 4.445183987829228 >500 0.003915273481852707 1.1892310408997677 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 602 0.40886184273081677 No Hit ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 584 0.39663673779866615 No Hit ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 565 0.38373246037028486 No Hit ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 369 0.2506146511090887 No Hit ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 354 0.2404270636656298 No Hit ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 316 0.21461850880886726 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 265 0.17998071150110706 No Hit ACCTGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 254 0.17250981404257054 No Hit ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG 253 0.17183064154633992 No Hit ACCTGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 252 0.17115146905010936 No Hit ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC 228 0.15485132914057512 No Hit ACCTGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 221 0.150097121666961 No Hit ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 211 0.14330539670465506 No Hit ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA 211 0.14330539670465506 No Hit ACCTGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC 207 0.14058870671973267 No Hit ACCTGAGGGGTTACATGGGACCACGAAATGCAATCTCTCTTTCTGAATCTGAATGTCTGCTCAT 205 0.1392303617272715 No Hit ACCTGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC 166 0.11274263437427838 No Hit ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT 158 0.10730925440443363 No Hit ACCTGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 151 0.10255504693081949 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0033958624811529633 0.0 0.0 0.0 0.0 7 0.0033958624811529633 0.0 0.0 0.0 0.0 8 0.004075034977383556 0.0 0.0 0.0 0.0 9 0.004075034977383556 0.0 0.0 0.0 0.0 10 0.008150069954767112 0.0 0.0 0.0 0.0 11 0.013583449924611853 0.0 0.0 0.0 0.0 12 0.014262622420842445 0.0 0.0 0.0 0.0 13 0.01562096741330363 0.0 0.0 0.0 0.0 14 0.017658484901995408 0.0 0.0 0.0 0.0 15 0.019696002390687188 0.0 0.0 0.0 0.0 16 0.019696002390687188 0.0 0.0 0.0 0.0 17 0.021733519879378965 0.0 0.0 0.0 0.0 18 0.023771037368070742 0.0 0.0 0.0 0.0 19 0.023771037368070742 0.0 0.0 0.0 0.0 20 0.029883589834146077 0.0 0.0 0.0 0.0 21 0.033958624811529635 0.0 0.0 0.0 0.0 22 0.04482538475121912 0.0 0.0 0.0 0.0 23 0.04550455724744971 0.0 0.0 0.0 0.0 24 0.0461837297436803 0.0 0.0 0.0 0.0 25 0.048221247232372075 0.0 0.0 0.0 0.0 26 0.05093793721729445 0.0 0.0 0.0 0.0 27 0.055012972194678 0.0 0.0 0.0 0.0 28 0.06587973213436749 0.0 0.0 0.0 0.0 29 0.06723807712682867 0.0 0.0 0.0 0.0 30 0.07267145709667341 0.0 0.0 0.0 0.0 31 0.07538814708159579 0.0 0.0 0.0 0.0 32 0.07742566457028756 0.0 0.0 0.0 0.0 33 0.08150069954767111 0.0 0.0 0.0 0.0 34 0.0835382170363629 0.0 0.0 0.0 0.0 35 0.09508414947228297 0.0 0.0 0.0 0.0 36 0.10255504693081949 0.0 0.0 0.0 0.0 37 0.10730925440443363 0.0 0.0 0.0 0.0 38 0.11342180687050897 0.0 0.0 0.0 0.0 39 0.11613849685543134 0.0 0.0 0.0 0.0 40 0.12157187682527608 0.0 0.0 0.0 0.0 41 0.1304011192762738 0.0 0.0 0.0 0.0 42 0.13379698175742674 0.0 0.0 0.0 0.0 43 0.14126787921596326 0.0 0.0 0.0 0.0 44 0.14534291419334683 0.0 0.0 0.0 0.0 45 0.14941794917073037 0.0 0.0 0.0 0.0 46 0.15145546665942217 0.0 0.0 0.0 0.0 47 0.1562096741330363 0.0 0.0 0.0 0.0 48 0.16028470911041987 0.0 0.0 0.0 0.0 49 0.16368057159157282 0.0 0.0 0.0 0.0 50 0.16911395156141756 0.0 0.0 0.0 0.0 51 0.17250981404257054 0.0 0.0 0.0 0.0 52 0.17726402151618467 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTACG 15 0.004590795 58.279644 34 GTAAAGT 15 0.004590795 58.279644 33 CACAAAG 20 1.9774272E-4 58.160053 57 TCACTAC 20 1.9841261E-4 58.120296 54 GATCAGC 15 0.0046471353 58.10045 53 AACGCGC 15 0.0046534273 58.0806 31 CAATCTC 45 3.092282E-11 58.0806 31 TAGTAAG 15 0.0046534273 58.0806 31 TTTAGGA 15 0.0046597254 58.060776 48 AAACTAA 15 0.0046597254 58.060776 48 GTTTAGG 15 0.0046597254 58.060776 47 TAAACTA 15 0.0046597254 58.060776 47 ACTAACT 15 0.0046597254 58.060776 50 CATACCG 15 0.0046597254 58.060776 46 ATCATCT 15 0.0046597254 58.060776 47 TACGCGC 20 1.9975784E-4 58.04096 45 TAATTGT 20 1.9975784E-4 58.04096 27 AGCGATG 20 1.9975784E-4 58.04096 30 TTACGCG 20 1.9975784E-4 58.04096 44 GCAATCT 45 3.092282E-11 58.04096 30 >>END_MODULE