FastQCFastQC Report
Fri 17 Jun 2016
SRR1526476_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526476_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133379
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7220.5413145997495858No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC6730.5045771823150571No Hit
ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.44084900921434406No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4660.3493803372344972No Hit
ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.31114343337406936No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC3420.25641217882875117No Hit
ACCTGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2990.2241732206719199No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC2880.21592604532947465No Hit
ACCTGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2790.20917835641292856No Hit
ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2460.18443683038559294No Hit
ACCTGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2450.18368708717264337No Hit
ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2450.18368708717264337No Hit
ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2450.18368708717264337No Hit
ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2420.18143785753379468No Hit
ACCTGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2270.17019170933955122No Hit
ACCTGAAAATTGACATCATCCCCAACCCTCAGGAGCGCACGCTGACTTTGGTGGAC2180.16344402042300513No Hit
ACCTGAGGGGTTACATGGGACCACGAAATGCAATCTCTCTTTCTGAATCTGAATGT2110.15819581793235815No Hit
ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1950.1461999265251651No Hit
ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1860.13945223760861905No Hit
ACCTGAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG1770.13270454869207296No Hit
ACCTGAGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG1700.127456346201426No Hit
ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.12670660298847644No Hit
ACCTGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC1660.12445737334962775No Hit
ACCTGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1570.11770968443308168No Hit
ACCTGAGGGAGTGTTTGTGAATCAATTAATTGTGATTGCACAAAAATAATTTTAAA1540.11546045479423298No Hit
ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1510.11321122515538429No Hit
ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1500.1124614819424347No Hit
ACCTGAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA1490.11171173872948514No Hit
ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1430.10721327945178777No Hit
ACCTGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1420.10646353623883821No Hit
ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC1360.1019650769611408No Hit
ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1350.10121533374819125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCA150.00799065250.65801634
AGTAGAT150.0080754250.5230333
CTATCAG251.9709621E-550.44621731
CCTTTTA150.00812415950.44621731
ATTTGTT251.9709621E-550.44621731
AAATCAC252.0293273E-550.19818538
TTACTTC150.008284070550.19818539
GCCGCAT150.008284070550.19818539
AGGCTAA150.008284070550.19818538
AATGCCG150.00830887850.1602446
CGATCCA252.0384323E-550.1602445
TCGCGCA451.3278623E-1050.1034450
ACATGCT150.00834619450.10343650
CGCATCT150.0083586650.08452641
CTTCTAT252.1031201E-549.89624428
ATGCAAT252.1031201E-549.89624428
CCATTTG252.1031201E-549.89624429
TTAATTG150.00853464649.82132326
GCTTTAA150.00853464649.82132326
TCAATTA150.00854732149.80262822
Produced by FastQC (version 0.11.5)