##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526476_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 133379 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.37507403714228 39.0 38.0 40.0 35.0 40.0 2 38.09344799406203 39.0 38.0 40.0 35.0 40.0 3 38.397596323259286 39.0 38.0 40.0 36.0 40.0 4 38.24360656475157 39.0 38.0 40.0 35.0 40.0 5 37.98106898387302 39.0 38.0 40.0 35.0 40.0 6 37.691593129353194 39.0 38.0 40.0 33.0 40.0 7 38.373904437730076 39.0 38.0 40.0 36.0 40.0 8 38.578456878519106 39.0 38.0 40.0 36.0 40.0 9 38.570134728855365 39.0 38.0 40.0 36.0 40.0 10 37.47674671425037 39.0 37.0 40.0 32.0 40.0 11 37.48955982575968 39.0 37.0 40.0 33.0 40.0 12 37.64768816680287 39.0 38.0 40.0 33.0 40.0 13 37.715180050832586 39.0 38.0 40.0 33.0 40.0 14 37.74523725623974 39.0 38.0 40.0 33.0 40.0 15 37.78293434498684 39.0 38.0 40.0 34.0 40.0 16 37.74504232300437 39.0 38.0 40.0 34.0 40.0 17 37.633143148471646 39.0 38.0 40.0 33.0 40.0 18 37.53846557554038 39.0 38.0 40.0 33.0 40.0 19 37.477219052474524 39.0 37.0 40.0 33.0 40.0 20 37.37699337976743 39.0 37.0 40.0 33.0 40.0 21 37.26338479070918 39.0 37.0 40.0 33.0 40.0 22 37.16897712533457 39.0 37.0 40.0 33.0 40.0 23 36.975071038169425 39.0 36.0 40.0 33.0 40.0 24 36.82939593189332 39.0 36.0 40.0 32.0 40.0 25 36.726928526979506 39.0 36.0 40.0 31.0 40.0 26 36.23144572983753 38.0 36.0 40.0 31.0 40.0 27 36.049730467314944 38.0 35.0 40.0 30.0 40.0 28 35.84907669123325 38.0 35.0 40.0 29.0 40.0 29 35.677805351667054 38.0 35.0 40.0 29.0 40.0 30 35.44075154259666 38.0 35.0 40.0 29.0 40.0 31 34.69408227682019 38.0 34.0 39.0 27.0 40.0 32 34.559653318738334 38.0 33.0 39.0 26.0 40.0 33 34.37895020955323 38.0 33.0 39.0 26.0 40.0 34 34.488727610793305 38.0 33.0 39.0 26.0 40.0 35 34.336634702614354 38.0 33.0 39.0 25.0 40.0 36 33.606324833744445 38.0 33.0 39.0 21.0 40.0 37 33.044497259688555 38.0 33.0 39.0 16.0 40.0 38 33.0563282075889 37.0 32.0 39.0 18.0 40.0 39 32.97168969627902 37.0 32.0 39.0 17.0 40.0 40 32.555034900546566 37.0 32.0 39.0 11.0 40.0 41 31.871793910585623 36.0 31.0 39.0 4.0 40.0 42 31.74402267223476 36.0 30.0 39.0 4.0 40.0 43 31.497319668013706 36.0 30.0 39.0 4.0 40.0 44 31.067844263339808 36.0 30.0 39.0 4.0 40.0 45 30.907609143868225 36.0 30.0 39.0 4.0 40.0 46 30.202378185471478 35.0 29.0 39.0 4.0 40.0 47 29.93880595895906 35.0 28.0 39.0 4.0 40.0 48 29.53889292917176 35.0 27.0 39.0 4.0 40.0 49 29.380374721657834 35.0 27.0 38.0 4.0 40.0 50 28.94963225095405 35.0 26.0 38.0 4.0 40.0 51 28.162394379924876 34.0 23.0 38.0 4.0 40.0 52 27.828203840184738 34.0 23.0 38.0 4.0 40.0 53 27.490751917468266 34.0 19.0 38.0 4.0 40.0 54 27.144692942667138 33.0 15.0 38.0 4.0 40.0 55 26.55672932020783 33.0 4.0 38.0 4.0 40.0 56 15.948665082209343 19.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.605935787651461 1 2 -0.8932913326095573 1 3 -0.603781426123696 1 4 -0.7705456676970783 1 5 -0.5841488194351427 1 6 0.14484861640473667 1 7 -0.38502386376859477 1 8 -0.9949443297032516 1 9 -0.8095137872918414 1 10 -0.054346456207738925 1 11 -0.8253874967018149 1 12 -0.5944231768886041 1 13 -0.22279656624034772 1 14 -0.019342722392686085 1 15 -0.128748381022028 1 16 -0.17979256731602788 1 17 -0.3181919194685605 1 18 -0.08508095588673825 1 19 -0.23130401989170224 1 20 -0.3230977907651251 1 21 -0.7159542608152236 1 22 -0.9323005338512829 1 23 -0.8355508817360544 1 24 -0.7149276728555876 1 25 -0.29943242090791244 1 26 0.2931258782017707 1 27 -0.3575590846090435 1 28 -0.4045641894954173 1 29 0.16060248262536447 1 30 -0.6150183956923314 1 31 -0.05522915637447312 1 32 0.1359846487621681 1 33 -0.2865800353253647 1 34 0.31912985986396336 1 35 0.5992664403788268 1 36 1.1780808310832285 1 37 1.049800564442478 1 38 1.4772845076821497 1 39 1.679495169167538 1 40 1.1645582480242282 1 41 0.8889480580531917 1 42 1.1980699406239026 1 43 0.6540655228786285 1 44 1.7020050905098607 1 45 1.865559503035911 1 46 1.740690064824154 1 47 2.6789783786426824 1 48 2.863444998995881 1 49 3.046903841433881 1 50 2.821625153527364 1 51 2.445345928811353 1 52 2.7091376060900245 1 53 2.761952811020933 1 54 2.9724792500297106 1 55 2.7257119068488755 1 56 2.939294682798355 2 1 0.050181797094303704 2 2 0.4734989216277299 2 3 0.2645978111644425 2 4 0.025932086540208843 2 5 -0.18240093807921198 2 6 -0.8221799852901768 2 7 0.11908100911276165 2 8 -0.21687229580494716 2 9 -0.3194449313596408 2 10 0.0997848997244617 2 11 -0.1764203356848668 2 12 -0.06449732943097786 2 13 -0.15632410861322654 2 14 -0.016297171545225808 2 15 -0.5279803725474537 2 16 -0.13490379612958492 2 17 -0.32706374150245665 2 18 -0.06283519317487674 2 19 -0.17039300294254645 2 20 -0.01258401957868216 2 21 -0.09691294725590183 2 22 -0.07848697452924824 2 23 -0.12275956817673261 2 24 -0.3915696220081273 2 25 0.19672753671920873 2 26 -0.9628328082389075 2 27 -0.9763355676598877 2 28 -0.23917753695304356 2 29 -0.24485302584920987 2 30 -0.8421105567092795 2 31 0.03971258091366536 2 32 -0.7215365376785101 2 33 -0.38549423024061724 2 34 -0.47284577572925457 2 35 -0.21694118673981677 2 36 -1.148852431628633 2 37 -0.20854159657447013 2 38 -0.5166243906229369 2 39 -0.9598056782900883 2 40 -0.8173008621452666 2 41 -0.29418223855697434 2 42 -1.7828622627659314 2 43 -1.3387840533925548 2 44 -1.0923540196596342 2 45 -1.0837254545912103 2 46 -0.7426256131419464 2 47 -0.9550258586454561 2 48 -1.1263590264278491 2 49 -1.4644838704305272 2 50 -1.8346248464726358 2 51 -0.4620958084767821 2 52 0.08241620778494152 2 53 -0.4060821466061846 2 54 -0.12378663980079807 2 55 -0.6559088558629895 2 56 -0.617114215506728 3 1 0.34154962496315733 3 2 0.5885091675293666 3 3 0.3524564177218181 3 4 0.530926963589387 3 5 0.5319945537240685 3 6 0.20458743274261337 3 7 0.7019447386209592 3 8 0.5971645894409576 3 9 0.342056093230525 3 10 1.039846375134296 3 11 0.007621672511852751 3 12 0.04436537548705388 3 13 -0.008014682383716831 3 14 -0.27872032728293306 3 15 -0.03399983976056831 3 16 0.22101321206713465 3 17 0.1059536765303335 3 18 -0.08230240628962804 3 19 -0.09918398654910732 3 20 0.2662325787819739 3 21 -0.19386479151819458 3 22 0.05880810743796161 3 23 -0.0019860026029618894 3 24 0.08307177143449707 3 25 -0.09284725836275953 3 26 0.8643956753676534 3 27 0.4384953749630611 3 28 1.038614471243676 3 29 1.0166736134950511 3 30 0.1503330908317011 3 31 1.1960906546841557 3 32 0.7482380524854264 3 33 0.8866625320544657 3 34 0.3359400849264844 3 35 0.3501694689978905 3 36 0.42940064214185725 3 37 1.4053159853927397 3 38 0.49131618314755343 3 39 1.1882476003984337 3 40 -0.3294678703419862 3 41 0.7530769827545036 3 42 -0.16605898407740582 3 43 0.3095253728369549 3 44 0.2981429065698755 3 45 1.0852038486874775 3 46 1.1444133212842829 3 47 1.0833962725020854 3 48 1.1365764243918228 3 49 1.2601575230120972 3 50 1.0101497436912972 3 51 1.1202556259494472 3 52 1.0428414127029733 3 53 1.3750397796233251 3 54 1.0074889749533007 3 55 0.9360481113501251 3 56 -0.02208347780180908 4 1 -0.09910357112275392 4 2 0.1930967898447875 4 3 -0.09550393302160387 4 4 -0.32369242896366757 4 5 0.42721630998280347 4 6 -0.05249733025141978 4 7 0.2791464160895032 4 8 -0.0015137686731634403 4 9 0.028483847710127463 4 10 -0.3287519219809667 4 11 0.3023223968732722 4 12 -0.2857909340821436 4 13 0.20401988363483525 4 14 -0.2462511444134421 4 15 -0.42884035316760816 4 16 -0.5966891643466425 4 17 -0.9340768228978078 4 18 -0.8491724024772012 4 19 -0.7307127703844074 4 20 -0.9531726823693774 4 21 -0.0054037418295465045 4 22 -0.4980606179400979 4 23 -0.9350778821302228 4 24 0.04653599814691489 4 25 0.003353021215332319 4 26 0.23623453672233552 4 27 0.6972957307897261 4 28 0.4767260483182767 4 29 -0.054809421198044106 4 30 0.5789771370891685 4 31 0.73211810416948 4 32 0.6139770282129646 4 33 0.25119423875162994 4 34 0.4655626545033016 4 35 0.5114987357408012 4 36 0.6786063862008263 4 37 0.5228658646658388 4 38 0.5816071985243525 4 39 0.7003202907021588 4 40 0.5347461339787642 4 41 0.7875159203577518 4 42 -0.022203348037248105 4 43 0.4828002489330281 4 44 1.1187046043713735 4 45 0.47750274308320684 4 46 1.1085129527495248 4 47 0.5970574746878761 4 48 1.0582945588434676 4 49 1.2507535326702488 4 50 1.4844158512017813 4 51 2.04559556749221 4 52 2.2320649480950188 4 53 2.4475394619209467 4 54 2.7457022167883487 4 55 2.8967800976253812 4 56 1.5513693116250558 5 1 0.3005660184057817 5 2 0.654082938021169 5 3 0.45901379477100335 5 4 0.39158270129430406 5 5 0.6303552094617686 5 6 -0.0822978131590304 5 7 0.46423982058816904 5 8 0.17093508124423096 5 9 0.18631838831249325 5 10 0.04804309644577387 5 11 -0.2874602947012619 5 12 -0.33268380484081206 5 13 -0.24571960041650698 5 14 -0.05740885187309175 5 15 -0.3454752495966318 5 16 -0.16423268957220927 5 17 0.19611761095656277 5 18 0.04884513469397689 5 19 0.10573404623777805 5 20 -0.04524283105408955 5 21 -0.11189757840344328 5 22 -0.07233943354564332 5 23 -0.34624829768493015 5 24 -0.6874200318441908 5 25 -0.158421028854562 5 26 -0.0864239967635001 5 27 -0.04511118241398293 5 28 0.0959915204240076 5 29 -0.34398212420986596 5 30 -0.34147018785682093 5 31 0.3846152448480922 5 32 0.2564347737968973 5 33 -0.03137025483078304 5 34 -0.3607812265489301 5 35 0.49771045260444424 5 36 -0.40666493162863304 5 37 -3.127470899853158 5 38 -0.6480280791475295 5 39 -0.24617862910976385 5 40 0.1951222935924406 5 41 -0.3452836729832036 5 42 0.11262954051275642 5 43 -0.010146758310586534 5 44 0.2571593000124963 5 45 -0.06233713491907977 5 46 0.570642829481006 5 47 -0.1379152029077524 5 48 0.16116658832624964 5 49 0.07982965415963506 5 50 0.03473990762572399 5 51 0.1694359538183008 5 52 -0.2522405545101414 5 53 -0.13315694168815284 5 54 -0.3531667627516164 5 55 0.263916963809141 5 56 -0.16142774009689198 6 1 -0.0682864406106134 6 2 0.39178785605395916 6 3 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1.8341155367713817 6 44 1.732569136078073 6 45 1.3229087667202677 6 46 1.316544468825267 6 47 1.935855288895528 6 48 1.3332977358672338 6 49 1.7273706377661924 6 50 1.846215317461791 6 51 1.3005834948019057 6 52 1.952677478276744 6 53 1.252088959951191 6 54 1.3271611061008421 6 55 1.3704743408583226 6 56 -0.3499523302608267 7 1 -0.2228978784782143 7 2 0.14131857811627668 7 3 -0.04348902089662943 7 4 -0.33517239819261846 7 5 -0.20589807548379468 7 6 -0.5865525997939969 7 7 -0.018741032871972152 7 8 0.06819449808818234 7 9 -0.2915942634207127 7 10 -0.051694107909128206 7 11 -0.4644093624024208 7 12 -0.6419066805760139 7 13 -0.18339949029261504 7 14 -0.4360848623849236 7 15 -0.5099102389596695 7 16 -0.773918586205923 7 17 -0.6241176537161977 7 18 -0.5995718343962508 7 19 0.029273027591806056 7 20 -0.6233306798840275 7 21 -0.5899353709200241 7 22 -0.7683724707124568 7 23 -0.4843411521462002 7 24 0.07825385127431872 7 25 -0.35163839839529487 7 26 -0.5503208807579938 7 27 -0.5053789836904201 7 28 0.26505671877215065 7 29 -0.011670773940814172 7 30 -0.06378278953370398 7 31 0.21498691297473727 7 32 -0.11967585065561082 7 33 0.6022801018204547 7 34 0.49787225862188933 7 35 -0.37796217910622687 7 36 0.10478545005075546 7 37 0.7308071629675155 7 38 -0.09665778756950516 7 39 0.15064279499235766 7 40 -0.4643070644353422 7 41 0.32884972709188176 7 42 0.6627484242569679 7 43 -0.1635693587360656 7 44 0.8517657322487615 7 45 -0.02116581718663113 7 46 1.2527226693008018 7 47 1.2179226146369864 7 48 1.4420450766255861 7 49 1.2209884578137462 7 50 1.0620831687945405 7 51 0.8163512335079517 7 52 0.6237449291589883 7 53 1.192021383928168 7 54 0.597527768596148 7 55 0.12951576245762197 7 56 0.1630623112108296 8 1 -0.018654324952940726 8 2 0.5299703783206411 8 3 0.21304761431404984 8 4 0.07397588575280878 8 5 0.4188539831806324 8 6 -0.3283930758413547 8 7 0.4683059106875618 8 8 0.2887871530139492 8 9 0.1224226867505962 8 10 0.45633017531500997 8 11 -0.024907048283289157 8 12 -0.5405061876986963 8 13 -0.44360265192031534 8 14 -0.3181549471357741 8 15 -0.6916737780592612 8 16 -0.524113933924859 8 17 -0.3640347060693827 8 18 -0.31923283097015087 8 19 -0.22329654624963524 8 20 -0.5532459290275042 8 21 -0.30797347875196834 8 22 -0.4712035099623222 8 23 -0.27402679258618434 8 24 -0.32476351964592 8 25 -0.3391361443831542 8 26 -0.10204294603418163 8 27 -0.4952209023055545 8 28 -0.43879613931525085 8 29 -1.0599366872665357 8 30 0.005760998408831597 8 31 -0.5927308245981422 8 32 0.17388669066794904 8 33 -0.7959888168547877 8 34 -0.20555985053240278 8 35 0.3029899156223905 8 36 0.45160278490679673 8 37 0.22176106090584824 8 38 0.7143057274873001 8 39 0.41691676265479316 8 40 0.4471553386421334 8 41 1.5204708126241293 8 42 0.6177282884151722 8 43 1.0392917340090229 8 44 1.008224228371862 8 45 0.5981766123379231 8 46 1.2668478120549054 8 47 0.6180548500159624 8 48 0.7411975680603398 8 49 1.179093885474984 8 50 0.33737318502342717 8 51 0.7938712190822734 8 52 0.5288359912495082 8 53 0.30165653449617835 8 54 0.2213782224039278 8 55 -0.24879763539054878 8 56 0.16450487898146093 9 1 0.042369297094303704 9 2 0.6099572549610599 9 3 0.21147281116444105 9 4 0.4077029198735431 9 5 0.4665573952541209 9 6 0.1585491813764932 9 7 0.6008518424460974 9 8 0.4643777041950514 9 9 0.4800342353070235 9 10 0.110201566391126 9 11 0.33972549764846605 9 12 0.19696100390235216 9 13 -0.4302824419465594 9 14 -0.29702633821189295 9 15 0.3277487941192163 9 16 -0.9302162961295863 9 17 -0.34008457483579235 9 18 -0.1336685265082096 9 19 -0.601643002942545 9 20 -0.4401881862453507 9 21 -0.37555878058923753 9 22 -0.5784869745292482 9 23 0.26317793182326454 9 24 -0.38427795534146014 9 25 -0.5985849632807927 9 26 -0.14147864157224177 9 27 -0.38623140099321773 9 28 -0.14594837028637642 9 29 -0.5375613591825399 9 30 0.3115352766240562 9 31 -0.44830825241966465 9 32 0.15138012898815845 9 33 -0.1360150635739501 9 34 0.24017505760407687 9 35 0.2934754799268475 9 36 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-0.7018342726134961 120 34 -1.5283277672546802 120 35 -0.7142928816550693 120 36 -0.40666493162863304 120 37 -0.15358926606599255 120 38 -0.4549188821483625 120 39 0.1625460166251642 120 40 -0.20832310790797948 120 41 0.2364056851718388 120 42 0.028578415200168905 120 43 0.5862689127091407 120 44 0.329123734577653 120 45 0.7214917064257378 120 46 0.910181590247884 120 47 0.40779193796471347 120 48 0.6939534735721509 120 49 1.2418190956711683 120 50 0.7029810857307552 120 51 1.199583216946948 120 52 0.37862913151375466 120 53 0.43991891271585004 120 54 1.0826487415551327 120 55 0.2935085170183669 120 56 0.18505739466276339 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 99.0 5 16.0 6 18.0 7 33.0 8 45.0 9 82.0 10 77.0 11 112.0 12 150.0 13 237.0 14 309.0 15 412.0 16 541.0 17 625.0 18 758.0 19 889.0 20 902.0 21 1110.0 22 1191.0 23 1326.0 24 1864.0 25 2689.0 26 2624.0 27 2419.0 28 2927.0 29 2962.0 30 3573.0 31 4281.0 32 4991.0 33 6185.0 34 8010.0 35 10652.0 36 14484.0 37 19804.0 38 22421.0 39 14561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.16504752402457 0.6083975364025777 0.15828838493334635 0.06826655463950008 8 99.28099625878136 0.5518110047308796 0.052482024906469533 0.1147107115812834 9 98.41579259103756 1.0024066757135681 0.25941115168054946 0.3223895815683129 10 60.06042930296373 24.32391905772273 5.77002376685985 9.845627872453685 11 34.90729425171878 26.16753761836571 18.560642979779427 20.36452515013608 12 36.74116615059342 16.20869852075664 25.130642754856463 21.919492573793477 13 26.13229968735708 17.15787342835079 29.56612360266609 27.143703281626042 14 24.803754714010452 19.287893896340503 31.645911275388176 24.262440114260865 15 26.676613259958465 19.918427938431087 28.949084938408593 24.455873863201855 16 29.401180095817182 20.25731187068429 24.696541434558664 25.64496659893986 17 31.35351142233785 19.749735715517435 20.334535421618096 28.56221744052662 18 30.709482002414173 22.273371370305668 22.597260438299884 24.419886188980275 19 31.046116705028524 20.312792868442557 21.86101260318341 26.780077823345504 20 27.498331821351186 23.694884502058045 22.36259081264667 26.4441928639441 21 29.123025363812893 20.181587806176385 25.97410386942472 24.721282960586 22 28.15285764625616 19.85020130605268 25.81515830827941 26.18178273941175 23 31.42923548684576 20.890844885626674 24.176219644771667 23.503699982755908 24 30.029165448315677 23.688492018863823 22.211475741694596 24.070866791125905 25 28.926326527550668 21.110427699185404 23.638218395115437 26.325027378148487 26 28.607332828172943 17.88417822658694 25.340045464434958 28.168443480805156 27 25.29384391371572 17.996907696233695 27.317351426812976 29.391896963237613 28 26.409018922022653 21.145979539296412 25.194586845403848 27.250414693277094 29 28.289108137736328 20.740790797555224 25.871363998137888 25.09873706657056 30 27.81973532926032 19.451270374461753 28.706479999398816 24.02251429687911 31 28.073102183747388 19.115679997595322 29.964531012819933 22.846686805837354 32 30.34644046893917 18.73651123092773 29.228987599732292 21.68806070040081 33 30.076121131888133 19.007717419102494 29.366058399650985 21.550103049358384 34 24.97366757952391 20.774775045893648 35.12820126997502 19.12335610460742 35 24.813359824196997 24.792287546283752 31.632499473193054 18.7618531563262 36 27.424867501806794 22.264966273187184 29.935557696940496 20.374608528065526 37 26.126331464344865 24.109191472550183 29.460880399933142 20.30359666317181 38 27.48137041418335 22.378107128595 30.63088178783746 19.509640669384186 39 26.76456405774389 20.224676772851932 30.607878002471296 22.402881166932882 40 26.25268959269186 20.026424068551584 31.15624174247858 22.56464459627798 41 22.7171979132113 20.809836856548355 32.01275594825246 24.460209281987876 42 22.03067734491652 19.661252130371174 33.53166523385065 24.77640529086165 43 22.72891893523269 18.796402486871493 35.74953220257137 22.72514637532444 44 24.493739197379444 18.8654323689911 33.699647523227995 22.941180910401464 45 23.59765993583695 19.8633704472542 31.636912625023587 24.90205699188526 46 22.270884145190397 20.84374740434786 31.93010805463895 24.955260395822794 47 23.02128944587045 20.28536916805073 33.95591121848105 22.737430167597765 48 23.719010139043256 21.679104195492247 33.47396435835205 21.12792130711245 49 25.422268574149225 20.825114683232442 32.2503608648665 21.50225587775183 50 22.1327952601191 21.225930292312807 32.8829237326562 23.758350714911884 51 21.934947678591666 23.050130331305958 32.75584602017302 22.259075969929356 52 22.170909420728023 23.090004837344296 32.72690168097714 22.01218406095054 53 24.422015574204277 21.289786043698495 32.04657140697059 22.241626975126636 54 24.394374716467564 21.13791017692424 31.079691516709513 23.388023589898683 55 24.20001209811572 21.474155400296404 30.175725130810875 24.150107370777 56 23.262250453720508 22.29053236539625 28.988959467634608 25.45825771324864 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 2.0 5 4.0 6 2.0 7 0.0 8 0.5 9 1.0 10 13.0 11 25.0 12 55.0 13 104.5 14 124.0 15 153.5 16 183.0 17 388.0 18 593.0 19 885.0 20 1177.0 21 1092.5 22 1192.5 23 1377.0 24 1641.5 25 1906.0 26 2428.0 27 2950.0 28 3262.5 29 3630.0 30 3685.0 31 3777.5 32 3870.0 33 3242.5 34 2615.0 35 2741.5 36 2868.0 37 2357.5 38 1705.5 39 1564.0 40 1734.0 41 1904.0 42 2094.0 43 2284.0 44 2391.5 45 2499.0 46 2612.0 47 2705.5 48 2686.0 49 2889.0 50 3092.0 51 3554.5 52 4017.0 53 4616.0 54 5766.5 55 6318.0 56 6840.5 57 7363.0 58 8472.5 59 9582.0 60 10531.5 61 11481.0 62 11153.0 63 10004.0 64 9183.0 65 8102.5 66 7022.0 67 6210.5 68 5399.0 69 4639.0 70 3879.0 71 3351.0 72 2395.0 73 1967.0 74 1736.0 75 1505.0 76 1212.0 77 919.0 78 735.0 79 448.0 80 345.0 81 269.0 82 193.0 83 138.0 84 83.0 85 61.5 86 40.0 87 27.5 88 10.0 89 5.0 90 3.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05847997061006606 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0014994864258991295 25 0.04573433598992345 26 0.06597740273956171 27 0.1087127658776869 28 0.1109619955165356 29 0.14769941295106426 30 0.23092090958846595 31 0.22867167994961726 32 0.2983977987539268 33 0.3246388112071616 34 0.34713110759564847 35 0.37712083611363106 36 0.41010953748341195 37 1.3172988251523854 38 0.4948305205467128 39 0.491081804481965 40 0.6927627287653978 41 0.5510612615179301 42 0.5795515036100136 43 0.6320335285164831 44 0.6657719730992135 45 0.6777678645064065 46 0.7092570794502883 47 0.68901401270065 48 0.8382129120776134 49 0.7932283193006395 50 0.7909790896617909 51 0.7677370500603543 52 0.8059739539207821 53 0.8314652231610674 54 0.838962655290563 55 0.8449606009941595 56 0.8539575195495542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 133379.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.84466820114111 #Duplication Level Percentage of deduplicated Percentage of total 1 70.72314197663584 29.59386410154522 2 11.239518383143409 9.40627834966524 3 5.57944528058482 7.004101095374835 4 3.2788647602666097 5.4881203187908145 5 2.2522038271339495 4.712136093388015 6 1.5175947824840537 3.8101950082096883 7 1.0123270981151007 2.9652344072155286 8 0.7453594209130653 2.4951454126961514 9 0.5823120475883322 2.192998897877477 >10 2.8273489572135024 20.330786705553347 >50 0.16125564394753816 4.635662285667159 >100 0.07525263384218447 5.878736532737537 >500 0.0053751881315846056 1.486740791278987 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCTGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 722 0.5413145997495858 No Hit ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 673 0.5045771823150571 No Hit ACCTGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 588 0.44084900921434406 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 466 0.3493803372344972 No Hit ACCTGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.31114343337406936 No Hit ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 342 0.25641217882875117 No Hit ACCTGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 299 0.2241732206719199 No Hit ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 288 0.21592604532947465 No Hit ACCTGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA 279 0.20917835641292856 No Hit ACCTGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 246 0.18443683038559294 No Hit ACCTGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 245 0.18368708717264337 No Hit ACCTGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 245 0.18368708717264337 No Hit ACCTGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT 245 0.18368708717264337 No Hit ACCTGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 242 0.18143785753379468 No Hit ACCTGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 227 0.17019170933955122 No Hit ACCTGAAAATTGACATCATCCCCAACCCTCAGGAGCGCACGCTGACTTTGGTGGAC 218 0.16344402042300513 No Hit ACCTGAGGGGTTACATGGGACCACGAAATGCAATCTCTCTTTCTGAATCTGAATGT 211 0.15819581793235815 No Hit ACCTGAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 195 0.1461999265251651 No Hit ACCTGAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 186 0.13945223760861905 No Hit ACCTGAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG 177 0.13270454869207296 No Hit ACCTGAGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG 170 0.127456346201426 No Hit ACCTGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 169 0.12670660298847644 No Hit ACCTGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC 166 0.12445737334962775 No Hit ACCTGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 157 0.11770968443308168 No Hit ACCTGAGGGAGTGTTTGTGAATCAATTAATTGTGATTGCACAAAAATAATTTTAAA 154 0.11546045479423298 No Hit ACCTGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT 151 0.11321122515538429 No Hit ACCTGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 150 0.1124614819424347 No Hit ACCTGAGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA 149 0.11171173872948514 No Hit ACCTGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 143 0.10721327945178777 No Hit ACCTGAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 142 0.10646353623883821 No Hit ACCTGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 136 0.1019650769611408 No Hit ACCTGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 135 0.10121533374819125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.002998972851798259 0.0 0.0 0.0 0.0 7 0.002998972851798259 0.0 0.0 0.0 0.0 8 0.002998972851798259 0.0 0.0 0.0 0.0 9 0.003748716064747824 0.0 0.0 0.0 0.0 10 0.007497432129495648 0.0 0.0 0.0 0.0 11 0.013495377833092166 0.0 0.0 0.0 0.0 12 0.01424512104604173 0.0 0.0 0.0 0.0 13 0.01424512104604173 0.0 0.0 0.0 0.0 14 0.01574460747194086 0.0 0.0 0.0 0.0 15 0.01574460747194086 0.0 0.0 0.0 0.0 16 0.01574460747194086 0.0 0.0 0.0 0.0 17 0.01574460747194086 0.0 0.0 0.0 0.0 18 0.019493323536688683 0.0 0.0 0.0 0.0 19 0.021742553175537378 0.0 0.0 0.0 0.0 20 0.025491269240285202 0.0 0.0 0.0 0.0 21 0.029239985305033026 0.0 0.0 0.0 0.0 22 0.038986647073377366 0.0 0.0 0.0 0.0 23 0.044984592776973885 0.0 0.0 0.0 0.0 24 0.04873330884172171 0.0 0.0 0.0 0.0 25 0.05173228169351997 0.0 0.0 0.0 0.0 26 0.05398151133236866 0.0 0.0 0.0 0.0 27 0.05473125454531823 0.0 0.0 0.0 0.0 28 0.06222868667481388 0.0 0.0 0.0 0.0 29 0.06447791631366256 0.0 0.0 0.0 0.0 30 0.0682266323784104 0.0 0.0 0.0 0.0 31 0.06897637559135995 0.0 0.0 0.0 0.0 32 0.07122560523020866 0.0 0.0 0.0 0.0 33 0.07422457808200691 0.0 0.0 0.0 0.0 34 0.07497432129495647 0.0 0.0 0.0 0.0 35 0.08247175342445212 0.0 0.0 0.0 0.0 36 0.08547072627625038 0.0 0.0 0.0 0.0 37 0.08846969912804864 0.0 0.0 0.0 0.0 38 0.0937179016186956 0.0 0.0 0.0 0.0 39 0.09446764483164516 0.0 0.0 0.0 0.0 40 0.10121533374819125 0.0 0.0 0.0 0.0 41 0.11021225230358603 0.0 0.0 0.0 0.0 42 0.11471071158128342 0.0 0.0 0.0 0.0 43 0.11845942764603123 0.0 0.0 0.0 0.0 44 0.12220814371077905 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTGCA 15 0.007990652 50.658016 34 AGTAGAT 15 0.00807542 50.52303 33 CTATCAG 25 1.9709621E-5 50.446217 31 CCTTTTA 15 0.008124159 50.446217 31 ATTTGTT 25 1.9709621E-5 50.446217 31 AAATCAC 25 2.0293273E-5 50.198185 38 TTACTTC 15 0.0082840705 50.198185 39 GCCGCAT 15 0.0082840705 50.198185 39 AGGCTAA 15 0.0082840705 50.198185 38 AATGCCG 15 0.008308878 50.16024 46 CGATCCA 25 2.0384323E-5 50.16024 45 TCGCGCA 45 1.3278623E-10 50.10344 50 ACATGCT 15 0.008346194 50.103436 50 CGCATCT 15 0.00835866 50.084526 41 CTTCTAT 25 2.1031201E-5 49.896244 28 ATGCAAT 25 2.1031201E-5 49.896244 28 CCATTTG 25 2.1031201E-5 49.896244 29 TTAATTG 15 0.008534646 49.821323 26 GCTTTAA 15 0.008534646 49.821323 26 TCAATTA 15 0.008547321 49.802628 22 >>END_MODULE