FastQCFastQC Report
Fri 17 Jun 2016
SRR1526474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209877
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6270.29874640861075774No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5940.28302291342071784No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC5290.2520523925918514No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4190.19964074195838516No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4020.19154075958775854No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3730.177723142602572No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3620.17248197753922534No Hit
TGCGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2510.11959385735454575No Hit
TGCGGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2390.11387622273998581No Hit
TGCGGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2360.1124468140863458No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2350.11197034453513247No Hit
TGCGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2320.1105409358814925No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC2310.11006446633027916No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2290.1091115272278525No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.10387036216450588No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG2140.10196448395965256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTC201.9626672E-458.2599237
CGGCGTA303.6326492E-758.25991436
ACTTCGA150.00459969958.25991436
GTAAATT150.00459969958.25991437
ATTACCG150.004617243558.20393857
TTACCGC150.004617243558.20393858
AAAGTAA150.004626034858.1759947
GTAGAAT201.9767093E-458.1759955
AATGTGC150.004626034858.1759949
TAACCCG150.00463043558.1620233
CCACGAC150.00463043558.1620253
CGGTAGG150.004665748758.0505531
ACCTAAC150.00467017758.03664430
GCGAACC150.004692365457.96722426
TAGAATA150.00471909557.88413613
ATAGAAT150.00471909557.88413612
TAGGGTA150.00474593857.80128510
GTATTGT202.0432996E-457.7875029
TACCAGT202.0432996E-457.78750221
TATAGCC303.8430153E-757.78750221
Produced by FastQC (version 0.11.5)