FastQCFastQC Report
Fri 17 Jun 2016
SRR1526473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197511
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12990.6576848884365935No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9630.48756778103498033No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC5430.27492139678296396No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC4680.2369488281665325No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4620.23391102267721797No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC3390.17163601014627033No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC3320.16809190374207006No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT3040.15391547812526898No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2960.14986507080618294No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2850.14429576074243966No Hit
TGCGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2830.14328315891266816No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG2580.13062563604052432No Hit
TGCGGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA2490.12606892780655254No Hit
TGCGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2330.1179681131683805No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG2320.11746181225349474No Hit
TGCGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT2290.1159429095088375No Hit
TGCGGAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG2280.11543660859395172No Hit
TGCGGAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCC2270.11493030767906598No Hit
TGCGGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA2250.11391770584929448No Hit
TGCGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG2230.11290510401952296No Hit
TGCGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.11239880310463721No Hit
TGCGGAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTCAGG2140.10834839578555118No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG2060.10429798846646515No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.1037916875515794No Hit
TGCGGAGGGAGTTCTTCTGATTACATTCTCTGTCTTCATCTAGAATTTGATTACTA2030.10277908572180791No Hit
TGCGGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1980.10024758114737914No Hit
TGCGGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC1980.10024758114737914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCATA150.00799293550.66357437
GCAAACT203.9122681E-450.6635737
CGAACCT150.00800925650.63744435
ATATGAT150.00800925650.63744435
TATATGA150.00801742650.6243934
TATGCGT150.00809123450.50720632
GTATGCG150.00811594850.4682631
AGATACG150.00821536950.31309548
TAAAGGT150.0082570650.24872244
ACCGCAA150.0082570650.24872244
ATAAGAC150.0082570650.24872243
ATACCTC150.0082570650.24872243
ATTACTA150.00826541750.23586750
CTCATGG150.00826541750.23586750
ATACGAT150.00826541750.23586750
GATTACT150.00826541750.23586749
TAACCGA204.0794798E-450.23586349
AATCTGG204.0794798E-450.23586350
ACTAAAA150.0082821550.2101840
AATCCTG150.00829052650.19734642
Produced by FastQC (version 0.11.5)