##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526473_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197511
Sequences flagged as poor quality	0
Sequence length	56
%GC	59
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.1267574970508	39.0	38.0	40.0	35.0	40.0
2	37.78147039911701	39.0	38.0	40.0	35.0	40.0
3	37.78489805631079	39.0	38.0	40.0	35.0	40.0
4	37.817083605470074	39.0	38.0	40.0	35.0	40.0
5	37.73599951395112	39.0	38.0	40.0	35.0	40.0
6	36.76797747973531	39.0	36.0	39.0	31.0	40.0
7	38.145500756919866	39.0	38.0	40.0	36.0	40.0
8	38.326234994506635	39.0	38.0	40.0	36.0	40.0
9	37.708846595885795	39.0	38.0	40.0	35.0	40.0
10	36.95138498615267	39.0	35.0	39.0	32.0	40.0
11	36.752018874898106	39.0	35.0	39.0	32.0	40.0
12	36.95653406645706	39.0	35.0	39.0	32.0	40.0
13	37.08367635220317	39.0	36.0	39.0	32.0	40.0
14	37.089589946889035	39.0	36.0	39.0	33.0	40.0
15	37.11751244234498	39.0	36.0	39.0	33.0	40.0
16	37.086440755198446	38.0	36.0	39.0	33.0	40.0
17	36.90100298211239	38.0	36.0	39.0	33.0	40.0
18	36.76056017133223	38.0	36.0	39.0	32.0	40.0
19	36.67066644389426	38.0	36.0	39.0	32.0	40.0
20	36.544010207026446	38.0	35.0	39.0	31.0	40.0
21	36.385851927234434	38.0	35.0	39.0	31.0	40.0
22	36.26393972993909	38.0	35.0	39.0	31.0	40.0
23	36.02911230260593	38.0	35.0	39.0	30.0	40.0
24	35.85598270476075	38.0	35.0	39.0	30.0	40.0
25	35.73206049283331	38.0	35.0	39.0	29.0	40.0
26	35.02795793651999	38.0	34.0	39.0	28.0	40.0
27	34.7555933593572	38.0	33.0	39.0	27.0	40.0
28	34.486114697409256	38.0	33.0	39.0	27.0	40.0
29	34.30626648642354	38.0	33.0	39.0	26.0	40.0
30	33.99892157905129	37.0	33.0	39.0	25.0	40.0
31	33.11960852813261	36.0	32.0	38.0	22.0	40.0
32	32.91919437398423	36.0	32.0	38.0	22.0	39.0
33	32.6862655750819	36.0	31.0	38.0	21.0	39.0
34	32.87463989347429	36.0	32.0	38.0	22.0	40.0
35	32.635351955080985	36.0	31.0	38.0	20.0	40.0
36	32.01138670757578	36.0	31.0	38.0	12.0	40.0
37	31.55111360886229	36.0	30.0	38.0	6.0	40.0
38	31.548182126565102	36.0	30.0	38.0	10.0	40.0
39	31.311121912197294	36.0	30.0	38.0	7.0	40.0
40	30.900572626334736	35.0	29.0	38.0	4.0	40.0
41	30.108677491380227	35.0	28.0	38.0	4.0	39.0
42	29.967399284090508	35.0	28.0	38.0	4.0	39.0
43	29.70118120003443	35.0	27.0	38.0	4.0	39.0
44	29.33994056027259	35.0	27.0	38.0	4.0	39.0
45	28.929836819215133	34.0	26.0	38.0	4.0	39.0
46	28.17279543924136	34.0	24.0	38.0	4.0	39.0
47	27.785237277923763	33.0	23.0	38.0	4.0	39.0
48	27.32293897555073	33.0	21.0	38.0	4.0	39.0
49	27.159378464996887	33.0	20.0	38.0	4.0	39.0
50	26.715858863556967	33.0	17.0	37.0	4.0	39.0
51	25.798299841527815	33.0	4.0	37.0	4.0	39.0
52	25.478732830070225	32.0	4.0	36.0	4.0	39.0
53	25.095792133096385	32.0	4.0	36.0	4.0	39.0
54	24.748084916789445	32.0	4.0	36.0	4.0	39.0
55	24.262167676736993	32.0	4.0	36.0	4.0	39.0
56	14.739761329748722	18.0	4.0	23.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-1.267226736834921
1	2	-0.857830509843069
1	3	-1.543451308860753
1	4	-2.394444522930854
1	5	-2.1728311667692637
1	6	-0.7665937653299935
1	7	-1.5799773483818385
1	8	-2.022318918996781
1	9	-1.9835469054159631
1	10	-0.7145382870502033
1	11	-0.7470220536105714
1	12	-0.5490258116207727
1	13	-1.2159261921961857
1	14	-1.4095785111886627
1	15	-1.177542258793899
1	16	-1.4437375188526431
1	17	-1.2039621737851292
1	18	-1.627135445519663
1	19	-1.1235519267639447
1	20	-1.4303911370639781
1	21	-1.0534684225458832
1	22	-1.283955577431854
1	23	-1.240878980185279
1	24	-1.1664611501779234
1	25	-0.845700813600331
1	26	-0.4714464524824393
1	27	-0.6981849669429963
1	28	-0.32732219682937114
1	29	-0.26567715063529107
1	30	-0.4440552313726016
1	31	0.6162895954651972
1	32	-0.32851768664291825
1	33	0.3126129704263647
1	34	0.49593886482698935
1	35	0.44250628711761664
1	36	0.14974655210735222
1	37	-0.1974986043744522
1	38	0.4118291902457578
1	39	-0.2738379266727975
1	40	0.18104081501086
1	41	0.3725789073489132
1	42	0.6835557688681462
1	43	0.431377456082906
1	44	0.16083619725875664
1	45	0.33180913227917586
1	46	0.1698886731586633
1	47	0.35130060959332
1	48	0.7843262235399635
1	49	1.0963440824211474
1	50	0.5979239682028741
1	51	1.3289449792627401
1	52	1.155194063723485
1	53	1.0796440564579477
1	54	0.6247720063723214
1	55	0.8772132082827966
1	56	1.4812122734164976
2	1	-0.33744465451046324
2	2	0.07831871444174254
2	3	-0.448840960013122
2	4	-0.2947225249037686
2	5	0.1231064173039158
2	6	-0.35730311875121146
2	7	0.12220182837361193
2	8	-0.07998190886316081
2	9	-0.08246180094103295
2	10	0.16672796709740823
2	11	-0.0583842632778655
2	12	-0.4953983342644861
2	13	-0.23126113973452078
2	14	-0.2225997648878888
2	15	-0.5301563741198834
2	16	-0.28070281344506043
2	17	-0.5210637790223274
2	18	-0.48015311209665157
2	19	-0.4687227634601996
2	20	-0.13597807993905775
2	21	0.02204050042066541
2	22	-0.08675353277217823
2	23	-0.08968177814062273
2	24	-0.061358468804051824
2	25	0.16568829948369768
2	26	-0.5248945737271669
2	27	-0.17581029202595033
2	28	-0.5683768107653435
2	29	-0.36055653365026785
2	30	0.5476047094398808
2	31	0.3433992717274137
2	32	-0.43093002634339683
2	33	0.13361557108998312
2	34	0.21739882357508833
2	35	-0.01978409042699525
2	36	0.3938502199398357
2	37	0.610680034312665
2	38	0.11831291367863273
2	39	0.08146653032657625
2	40	0.418418628659861
2	41	0.26137811818202294
2	42	-0.11422021534851723
2	43	0.27435118041256246
2	44	0.2767002453261931
2	45	0.5583359011788254
2	46	1.1268432063843825
2	47	1.0376067704757546
2	48	1.511525577857963
2	49	1.0957163360306907
2	50	0.8606806716375424
2	51	0.6554627803568138
2	52	1.2585928620375384
2	53	1.2360425857378345
2	54	0.753101304193148
2	55	0.48020845534583856
2	56	-0.11550550974196483
3	1	0.106665621178756
3	2	0.5347653866778899
3	3	0.15104765040180723
3	4	0.18845764775008433
3	5	0.3952033262429282
3	6	-0.5430730992580379
3	7	0.17934468551646887
3	8	0.05106793797615694
3	9	0.04187682339759391
3	10	-0.02143688948850553
3	11	0.006917880971641921
3	12	-0.4394807625574373
3	13	-0.019732317679384437
3	14	-0.44799659028470984
3	15	0.035452091488586746
3	16	-0.6057654700866593
3	17	-0.30182122820082924
3	18	-0.49198825551074066
3	19	-0.18543120122127021
3	20	-0.4486486452411995
3	21	0.09369185101938626
3	22	0.16495908209832777
3	23	0.06328396956196514
3	24	0.43452011654821376
3	25	-0.22333982638653538
3	26	0.20428949756216497
3	27	0.5476372156320863
3	28	1.0743132477141941
3	29	0.2399725668788335
3	30	0.7079221697573459
3	31	1.2524775229109295
3	32	1.3409441034254712
3	33	1.1302460361415
3	34	1.7282314607235136
3	35	0.9938889811408131
3	36	1.0189407239777069
3	37	1.5418412707370592
3	38	1.374035553611801
3	39	0.8537862184357401
3	40	-0.11973230422791659
3	41	0.8492366534089797
3	42	0.19753138587676844
3	43	1.0222487019942967
3	44	0.9418353051980972
3	45	0.8504829354311241
3	46	0.9673611679549765
3	47	1.2229033452460136
3	48	1.2280669312414219
3	49	0.9154044451924825
3	50	1.2310788894041806
3	51	1.0742319254417474
3	52	1.8495146108540226
3	53	2.128885804896843
3	54	1.4503444119625684
3	55	1.4129848407538041
3	56	0.4022054342847685
4	1	0.14080502373921178
4	2	0.5707540068770385
4	3	0.34621123503907825
4	4	0.28248325230200777
4	5	0.68097857517607
4	6	0.12477896333086846
4	7	0.5469122530840309
4	8	0.33840790952665856
4	9	0.42295790447867176
4	10	0.5721619725314113
4	11	-0.4023282072218137
4	12	0.18839974953360183
4	13	-0.06781197628535551
4	14	-0.015564157852281824
4	15	-0.29157493553844205
4	16	-0.49125622399846947
4	17	-1.043357501316045
4	18	-0.46396549590903646
4	19	-0.5081908029282403
4	20	-0.6244665684275432
4	21	0.21417549255566115
4	22	0.6089135628949123
4	23	-0.34510863356747734
4	24	0.1934105859649975
4	25	0.2016957354911284
4	26	-0.09883994767254478
4	27	0.019756703541048637
4	28	0.11030186791333563
4	29	0.42645905336532053
4	30	-0.07856431672914255
4	31	1.48988861821676
4	32	0.9440735486601781
4	33	0.7150255524999665
4	34	1.416140422316687
4	35	0.5240454534025432
4	36	1.182667608757221
4	37	1.4772609008935262
4	38	1.6245334198991408
4	39	1.4654505143105574
4	40	1.6346062448474754
4	41	2.0463917031956065
4	42	2.129394828266527
4	43	1.9100154160767993
4	44	2.544253512879461
4	45	1.379785922628848
4	46	2.468499148040326
4	47	1.033145166014151
4	48	1.2219502882826738
4	49	2.322943563257919
4	50	1.5282339391908124
4	51	2.3891679140619466
4	52	2.8526440560887316
4	53	2.346524496219743
4	54	2.0617242128160562
4	55	2.3833973585347437
4	56	0.7630873688509148
5	1	-0.3485377281035369
5	2	-0.020677197054162377
5	3	-0.5211413448135076
5	4	-0.439353357034598
5	5	-0.161435429737935
5	6	-0.9376903241384156
5	7	-0.11724622357444048
5	8	-0.23732919121044205
5	9	-0.3444868129660392
5	10	-0.39141296604352505
5	11	-0.0827950376886406
5	12	-0.1381051644713125
5	13	-0.5578304038037842
5	14	-0.2468602266483444
5	15	-0.01341154487504781
5	16	-0.12215302989527288
5	17	-0.45020639566494225
5	18	-0.21178490622844492
5	19	-0.27400775624519724
5	20	-0.509294578255556
5	21	0.1187516596318261
5	22	-0.2102203437389889
5	23	-0.14550789646673934
5	24	-0.03760907005465697
5	25	-0.13601925222385347
5	26	0.4405335167009241
5	27	0.5292161630005054
5	28	0.559779754891224
5	29	-0.46457288766661975
5	30	0.05905853339370992
5	31	0.7387215420496887
5	32	0.1797000842867149
5	33	0.16954029451471087
5	34	-0.3664455727693081
5	35	0.6633267801838727
5	36	0.6780914416810546
5	37	-1.5368668632342342
5	38	0.9726599555256747
5	39	0.6801618165218635
5	40	0.9879231981644274
5	41	0.3863179931218994
5	42	1.1185840174557171
5	43	0.41465300821439044
5	44	1.0103161664421165
5	45	0.5605905909335185
5	46	0.6945740866152654
5	47	0.6530468859158702
5	48	0.3305190843514687
5	49	-0.23172474141038535
5	50	-0.6784306799738076
5	51	0.5341003464943803
5	52	0.4996342280789037
5	53	0.16034513504038372
5	54	-0.026390037798194044
5	55	0.1906762283136132
5	56	-0.4322443264807809
6	1	0.38046424818104896
6	2	0.9444907871355568
6	3	0.6169515405619919
6	4	0.6834233228072932
6	5	1.1204321591948698
6	6	0.32466145451770956
6	7	0.5010838159512474
6	8	0.4879672514773006
6	9	0.6560072581802032
6	10	0.7632313027311781
6	11	-0.025976855870460724
6	12	0.6235055074654454
6	13	0.2961715724808016
6	14	-0.3143009381107973
6	15	-0.031939212859242616
6	16	-0.11869453187156154
6	17	0.2056730509753777
6	18	-0.12556949120867955
6	19	0.06319580106706013
6	20	-0.3259941829986346
6	21	0.24449276635119332
6	22	0.026686770885511635
6	23	0.11511463317752657
6	24	-0.4150794257858834
6	25	0.34817047109630295
6	26	-0.17013842005797386
6	27	-0.18274035873862715
6	28	-0.4029178735672758
6	29	0.21823343644405213
6	30	0.20357434367038962
6	31	-0.649124307752686
6	32	-0.8183236311283046
6	33	0.35209958305225797
6	34	-0.12657403126733158
6	35	0.4367951596305133
6	36	0.008872074092124649
6	37	0.08566278103453939
6	38	0.41757102271935054
6	39	0.19177248845862138
6	40	0.4795239886782632
6	41	0.7371085069558916
6	42	1.156627495716588
6	43	0.8422715852894882
6	44	1.0110572731614837
6	45	0.7130115395501164
6	46	0.841560252623168
6	47	0.7059124985640892
6	48	0.686991416367281
6	49	0.9125440332931696
6	50	-0.029962300527166974
6	51	0.748774259537857
6	52	1.1261164415176381
6	53	0.07734118247121202
6	54	0.6369004760357981
6	55	0.4522869002503711
6	56	0.15174776838087922
7	1	0.16456035802086433
7	2	0.7137127550989462
7	3	0.5299950188228593
7	4	0.5674050161711435
7	5	0.6530980630850323
7	6	-0.05359941504750765
7	7	0.48987100130594285
7	8	0.40896267481826243
7	9	0.3471399812923295
7	10	0.6680367947220205
7	11	0.3648126178137474
7	12	-0.3026386572942812
7	13	0.1328992612679869
7	14	0.1572665676100229
7	15	-0.05402159272193785
7	16	0.1468661088607064
7	17	0.17712614022022422
7	18	-0.13935667656337358
7	19	0.15141090404188873
7	20	0.2513513547588033
7	21	-0.01157130687535357
7	22	-0.15083039158588463
7	23	-0.452505504122243
7	24	0.4082043270745288
7	25	-0.3707082474391683
7	26	0.15165791861479505
7	27	0.5476372156320863
7	28	0.6743132477141884
7	29	0.76102519845778
7	30	0.5131853276520815
7	31	0.989319628174087
7	32	0.08831252447810556
7	33	0.7723512992993946
7	34	0.7545472501971986
7	35	0.3780995074565965
7	36	1.0031512502934987
7	37	0.9155254812633729
7	38	0.7161408167696983
7	39	0.9801020079094194
7	40	0.8907940115615567
7	41	0.6597629691984537
7	42	0.8554261227188746
7	43	0.8906697546258755
7	44	0.7260458315138862
7	45	-0.40214864351624513
7	46	0.7410453784812923
7	47	0.21237702945654036
7	48	-0.4035120161269994
7	49	0.12593076098195866
7	50	0.6573946788778677
7	51	0.35317929386280156
7	52	0.12319882138033833
7	53	0.28151738384421066
7	54	-0.19702400909006101
7	55	-0.08701515924619585
7	56	0.665363329021611
8	1	0.14350772644191778
8	2	0.6142975504205808
8	3	0.3133283521561907
8	4	0.33728805710681087
8	5	0.5437413379388332
8	6	-0.07582163726973334
8	7	0.21823357440535318
8	8	0.06393343505218496
8	9	-0.2525676210468504
8	10	0.1098496602190977
8	11	-0.5176435225371279
8	12	-0.47661526548141353
8	13	-0.1852293937027767
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115	21	-0.05442027712707187
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115	23	9.841983949172572E-4
115	24	-0.43138377361196945
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116	4	0.17990134078154796
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116	6	-0.877063102198349
116	7	-2.9200966552656595
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116	34	1.191800999094582
116	35	1.0585464348309017
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116	37	1.4642758488023304
116	38	1.0910893613154151
116	39	1.8445536397824007
116	40	1.3987916593122165
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116	48	0.9784050670838198
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117	1	0.23100772644191636
117	2	0.8083947726428065
117	3	0.23312001882285927
117	4	0.15534361266237084
117	5	0.566310782383276
117	6	-0.7265160817141734
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118	34	0.37507632156672344
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118	41	-0.06644259707324807
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119	1	0.7456353860163816
119	2	1.1914843116498943
119	3	0.9714843805249842
119	4	0.9722762367758406
119	5	1.2949233710357575
119	6	0.5325707977184493
119	7	0.8144510684951882
119	8	0.6173613310332726
119	9	0.9142172489295106
119	10	1.2345541519448702
119	11	0.09370399519336559
119	12	0.6342594390103145
119	13	0.31559802946507176
119	14	0.5637055373748652
119	15	0.4780052829779464
119	16	0.3935626374161387
119	17	0.41161662174318536
119	18	0.34563772433248374
119	19	0.11731235980896315
119	20	0.669380470100343
119	21	0.5400524333262169
119	22	0.6907709521991094
119	23	0.7823209236493156
119	24	0.7194025353612048
119	25	1.0163018309482936
119	26	1.1477385457144607
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119	28	1.2342236620478957
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119	30	1.082390254863725
119	31	1.669330826382378
119	32	1.3421759063370047
119	33	2.0625528670485593
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119	35	1.2536314223502174
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119	37	2.6164213379263046
119	38	2.343912373320645
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119	40	2.504847762961333
119	41	2.8747125772611604
119	42	2.98560529405146
119	43	3.1036036851969833
119	44	3.4342765146045906
119	45	2.268400068689804
119	46	1.9741920750098494
119	47	1.7226793810802796
119	48	1.8299146356087235
119	49	1.4853932469841986
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119	51	1.822664176281613
119	52	1.874150669084706
119	53	1.0358286940345813
119	54	1.995249227192133
119	55	2.266232321156938
119	56	0.760212153209741
120	1	0.09268805431076999
120	2	0.33870556499253013
120	3	0.14201687674635366
120	4	-0.05333125072561273
120	5	0.08381419768382159
120	6	0.013067251619155229
120	7	0.2634976909809481
120	8	-0.05482794928297352
120	9	0.028215621211799657
120	10	-0.032682215919336954
120	11	0.2834493736377368
120	12	-0.45575916347777223
120	13	0.06304018735369255
120	14	-0.2665758252573909
120	15	-0.3596298757245293
120	16	-0.37594090868315533
120	17	-0.34585343987755834
120	18	-0.16052434984493402
120	19	-0.6815773041835982
120	20	-0.1608718261442732
120	21	-0.6210334869156142
120	22	-0.5564386745884704
120	23	-0.5125285124627652
120	24	-0.7132797108892319
120	25	-0.6239725557509317
120	26	-0.02910135662247626
120	27	-0.7972865692399296
120	28	-0.8507083226050511
120	29	-0.43489082109930877
120	30	-1.0194002346142383
120	31	-0.013700216519609398
120	32	-0.04806941397458786
120	33	-0.17872721666263658
120	34	0.3903539801368012
120	35	0.2556951358719033
120	36	0.2921935281192063
120	37	0.5258504740732661
120	38	0.6072538452425178
120	39	0.7183821344552932
120	40	0.5741129646820653
120	41	0.4080517238720205
120	42	0.3098408480568118
120	43	0.7792518656411183
120	44	0.7070064297307397
120	45	-0.1850937111032458
120	46	0.8853651944145682
120	47	0.6537920423987309
120	48	0.8948198791850501
120	49	1.2353641805965694
120	50	0.6297846702497374
120	51	1.3254254831064038
120	52	1.5446540989184463
120	53	0.9472924773155924
120	54	2.048963911531164
120	55	2.133951191055786
120	56	1.7632350575231914
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	123.0
5	24.0
6	28.0
7	32.0
8	78.0
9	104.0
10	122.0
11	247.0
12	275.0
13	383.0
14	579.0
15	797.0
16	1000.0
17	1321.0
18	1740.0
19	1867.0
20	1939.0
21	2420.0
22	2556.0
23	2869.0
24	3978.0
25	4926.0
26	4279.0
27	4773.0
28	5283.0
29	5952.0
30	6871.0
31	8120.0
32	9510.0
33	11547.0
34	14715.0
35	19014.0
36	24002.0
37	27342.0
38	21093.0
39	7602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.26802828602112	0.4862926265880494	0.13069114339553828	0.11498794399529917
8	99.4845856686463	0.3777004825047719	0.10986729853020845	0.02784655031871643
9	99.03549675714264	0.5366789697788984	0.16454779733786978	0.26327647574059165
10	69.8350977920217	18.956412554237488	5.000734136326584	6.20775551741422
11	40.698492742176384	25.678569801175634	14.453878518158483	19.169058938489503
12	34.72262304377984	16.545407597551527	24.565720390256747	24.166248968411885
13	27.21823088334321	15.810764970052302	28.633341940448886	28.337662206155606
14	25.558576484347707	17.203598786903008	31.23471604113189	26.0031086876174
15	26.927614158198782	17.257772984795782	29.291533129800367	26.52307972720507
16	33.367255494630676	18.265818106333317	23.060487770301403	25.3064386287346
17	34.27049632678686	18.549852919584225	21.14818921477791	26.031461538851
18	33.89279584428209	20.227227850600727	20.830232240229655	25.049744064887523
19	33.68571877009382	20.507212256532547	20.2814020484935	25.525666924880134
20	31.632162259317205	21.901058675213026	21.68233667998238	24.78444238548739
21	31.871642592058162	19.947243444668906	24.210803448921833	23.970310514351098
22	31.23927274936586	18.54428360952048	25.64312873713363	24.57331490398003
23	32.59395774369776	18.954579284994608	24.26522335691032	24.186239614397316
24	31.717381398410204	20.569591413093008	21.7680117462407	25.945015442256086
25	30.32605447242188	19.792220685742652	22.07009386032894	27.811630981506525
26	28.445214466278617	18.562592136658612	24.383855884334615	28.608337512728156
27	27.609277430865298	17.571567593869112	25.520030816640986	29.299124158624608
28	28.831806017354634	20.26700997486011	22.417585759468007	28.48359824831725
29	31.00519710989986	21.38217771580682	22.43021929268602	25.182405881607302
30	30.355928591001817	18.73978747805259	24.54608194375374	26.358201987191848
31	32.343150587614424	17.623358434651998	25.046684393204377	24.986806584529198
32	34.859641282931484	18.25425037070139	23.555280209624016	23.330828136743108
33	35.221176900881865	18.47086194985167	24.266976876498557	22.04098427276791
34	29.673791004739964	20.515451871345327	28.982355959499483	20.82840116441523
35	29.664974516111002	24.019899284011974	26.372141002383238	19.942985197493787
36	30.6787104907333	24.105100495104868	23.90939682604231	21.306792188119516
37	31.34591014268197	24.697166369947002	22.489469444715226	21.467454042655802
38	31.99963372013166	22.953538416144802	23.034425220403822	22.012402643319717
39	31.926497222278748	21.262286074764454	23.107486620133898	23.703730082822897
40	30.750958323138406	19.885714868281543	23.442215153739497	25.92111165484055
41	27.460443521290244	20.532714276986987	25.293746309080174	26.713095892642595
42	26.318040851245854	18.81387781291535	26.059346848567255	28.808734487271543
43	25.624302579754303	17.81370535873514	28.687601587697888	27.874390473812667
44	27.68767329962486	17.990030174522918	26.09127793182189	28.23101859403034
45	27.271105402126594	18.13010367923663	26.150717191179616	28.448073727457157
46	27.23865565929499	19.712305786008944	25.36726649703484	27.681772057661224
47	26.13410418769692	19.124529687069835	27.8547612492735	26.886604875959748
48	27.804865596201466	19.748296020218007	28.221479079978558	24.22535930360197
49	29.676566511338294	18.78384516840284	26.410587508740413	25.12900081151846
50	26.73090467392747	18.991721871204156	26.493584705365986	27.783788749502392
51	26.05813828891576	20.59128180793036	27.38632826651563	25.964251636638245
52	26.03517369885392	20.503867064859485	27.045970850243766	26.414988386042833
53	26.596782182009427	19.63573605927076	27.468891532676015	26.2985902260438
54	26.83483661399226	18.638466960172426	27.152517441801077	27.37417898403424
55	27.636047907249928	18.510687198345206	24.89593707704487	28.957327817359996
56	26.604220719524402	20.507058366871643	23.030603280965902	29.858117632638052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.0
9	3.0
10	4.5
11	6.0
12	23.0
13	61.0
14	82.0
15	97.0
16	112.0
17	136.0
18	160.0
19	250.0
20	340.0
21	546.0
22	709.0
23	666.0
24	797.0
25	928.0
26	1219.0
27	1510.0
28	1738.0
29	2207.0
30	2448.0
31	2573.5
32	2699.0
33	2717.0
34	2735.0
35	2600.5
36	2466.0
37	2522.0
38	2159.0
39	1740.0
40	1863.0
41	1986.0
42	1959.5
43	1933.0
44	2145.5
45	2358.0
46	2548.5
47	2988.0
48	3237.0
49	3339.5
50	3442.0
51	3976.0
52	4510.0
53	5276.5
54	6659.5
55	7276.0
56	8283.0
57	9290.0
58	10519.0
59	11748.0
60	13133.5
61	14519.0
62	17622.5
63	21184.5
64	21643.0
65	19047.5
66	16452.0
67	15103.0
68	13754.0
69	11949.5
70	10145.0
71	8881.0
72	6668.5
73	5720.0
74	4770.0
75	3820.0
76	3396.0
77	2972.0
78	2390.0
79	1464.0
80	1120.0
81	878.0
82	636.0
83	524.5
84	413.0
85	303.0
86	193.0
87	147.5
88	71.5
89	41.0
90	32.0
91	23.0
92	14.5
93	6.0
94	4.5
95	3.0
96	2.5
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.05012379057368957
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0010126018297715064
24	5.063009148857532E-4
25	0.04556708233971779
26	0.05771830429697586
27	0.10885469670043693
28	0.10885469670043693
29	0.14480206165732543
30	0.22732911078370321
31	0.22429130529438868
32	0.2971986370379371
33	0.3316270992501683
34	0.34124681663299766
35	0.3645366587177423
36	0.3974462181853162
37	1.3173949805327299
38	0.475922859992608
39	0.47946696639680825
40	0.6759117213724806
41	0.5483238908212706
42	0.5771830429697586
43	0.633888745436963
44	0.6678109067343085
45	0.6728739158831659
46	0.7088212808400545
47	0.691100748819053
48	0.8333713059019497
49	0.8009680473492615
50	0.7964113391152898
51	0.7746403997752024
52	0.8232452876042348
53	0.8424847223698934
54	0.8693186708588383
55	0.8688123699439524
56	0.8683060690290668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	197511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.735903316777296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50130408938168	30.25907417814704
2	10.562517438404523	8.816724131820507
3	4.916720245532736	6.156112824095874
4	2.947848555796829	4.921244892689521
5	2.093821649096842	4.36937689546405
6	1.4387441922531026	3.60283731032702
7	1.122123421445295	3.278298423885252
8	0.7048148193078015	2.353286652388981
9	0.5871434983562409	2.205446785242341
>10	2.856865575679643	20.96794608907858
>50	0.18075285383280967	5.269073621216033
>100	0.0837043417078112	6.380404129390262
>500	0.002426212803124962	0.7624891778179443
>1k	0.001213106401562481	0.6576848884365935
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1299	0.6576848884365935	No Hit
TGCGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	963	0.48756778103498033	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	543	0.27492139678296396	No Hit
TGCGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	468	0.2369488281665325	No Hit
TGCGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	462	0.23391102267721797	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	339	0.17163601014627033	No Hit
TGCGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	332	0.16809190374207006	No Hit
TGCGGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	304	0.15391547812526898	No Hit
TGCGGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	296	0.14986507080618294	No Hit
TGCGGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	285	0.14429576074243966	No Hit
TGCGGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	283	0.14328315891266816	No Hit
TGCGGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	258	0.13062563604052432	No Hit
TGCGGAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA	249	0.12606892780655254	No Hit
TGCGGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	233	0.1179681131683805	No Hit
TGCGGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	232	0.11746181225349474	No Hit
TGCGGAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT	229	0.1159429095088375	No Hit
TGCGGAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG	228	0.11543660859395172	No Hit
TGCGGAGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCC	227	0.11493030767906598	No Hit
TGCGGAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	225	0.11391770584929448	No Hit
TGCGGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG	223	0.11290510401952296	No Hit
TGCGGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	222	0.11239880310463721	No Hit
TGCGGAGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTCAGG	214	0.10834839578555118	No Hit
TGCGGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG	206	0.10429798846646515	No Hit
TGCGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.1037916875515794	No Hit
TGCGGAGGGAGTTCTTCTGATTACATTCTCTGTCTTCATCTAGAATTTGATTACTA	203	0.10277908572180791	No Hit
TGCGGAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	198	0.10024758114737914	No Hit
TGCGGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC	198	0.10024758114737914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0030378054893145192	0.0	0.0	0.0	0.0
11	0.010632319212600817	0.0	0.0	0.0	0.0
12	0.01265752287214383	0.0	0.0	0.0	0.0
13	0.013163823787029584	0.0	0.0	0.0	0.0
14	0.015189027446572596	0.0	0.0	0.0	0.0
15	0.01569532836145835	0.0	0.0	0.0	0.0
16	0.016201629276344102	0.0	0.0	0.0	0.0
17	0.016707930191229854	0.0	0.0	0.0	0.0
18	0.018226832935887116	0.0	0.0	0.0	0.0
19	0.020252036595430126	0.0	0.0	0.0	0.0
20	0.02227724025497314	0.0	0.0	0.0	0.0
21	0.02531504574428766	0.0	0.0	0.0	0.0
22	0.029365453063373684	0.0	0.0	0.0	0.0
23	0.0313906567229167	0.0	0.0	0.0	0.0
24	0.03544106404200272	0.0	0.0	0.0	0.0
25	0.036959966786659984	0.0	0.0	0.0	0.0
26	0.037466267701545736	0.0	0.0	0.0	0.0
27	0.0399977722759745	0.0	0.0	0.0	0.0
28	0.04404817959506053	0.0	0.0	0.0	0.0
29	0.04556708233971779	0.0	0.0	0.0	0.0
30	0.047085985084375045	0.0	0.0	0.0	0.0
31	0.04860488782903231	0.0	0.0	0.0	0.0
32	0.05012379057368957	0.0	0.0	0.0	0.0
33	0.053161596063004087	0.0	0.0	0.0	0.0
34	0.056705702467204355	0.0	0.0	0.0	0.0
35	0.05873090612674737	0.0	0.0	0.0	0.0
36	0.06024980887140463	0.0	0.0	0.0	0.0
37	0.06328761436071915	0.0	0.0	0.0	0.0
38	0.06835062350957669	0.0	0.0	0.0	0.0
39	0.07240103082866271	0.0	0.0	0.0	0.0
40	0.07290733174354846	0.0	0.0	0.0	0.0
41	0.07543883631797722	0.0	0.0	0.0	0.0
42	0.07746403997752024	0.0	0.0	0.0	0.0
43	0.0810081463817205	0.0	0.0	0.0	0.0
44	0.08455225278592078	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCATA	15	0.007992935	50.663574	37
GCAAACT	20	3.9122681E-4	50.66357	37
CGAACCT	15	0.008009256	50.637444	35
ATATGAT	15	0.008009256	50.637444	35
TATATGA	15	0.008017426	50.62439	34
TATGCGT	15	0.008091234	50.507206	32
GTATGCG	15	0.008115948	50.46826	31
AGATACG	15	0.008215369	50.313095	48
TAAAGGT	15	0.00825706	50.248722	44
ACCGCAA	15	0.00825706	50.248722	44
ATAAGAC	15	0.00825706	50.248722	43
ATACCTC	15	0.00825706	50.248722	43
ATTACTA	15	0.008265417	50.235867	50
CTCATGG	15	0.008265417	50.235867	50
ATACGAT	15	0.008265417	50.235867	50
GATTACT	15	0.008265417	50.235867	49
TAACCGA	20	4.0794798E-4	50.235863	49
AATCTGG	20	4.0794798E-4	50.235863	50
ACTAAAA	15	0.00828215	50.21018	40
AATCCTG	15	0.008290526	50.197346	42
>>END_MODULE