FastQCFastQC Report
Fri 17 Jun 2016
SRR1526472_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526472_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182420
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12740.6983883346124329No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.4681504221028396No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3700.20282863721083214No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3600.1973467821510799No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3560.19515404012717905No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3520.19296129810327814No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3010.16500383729854184No Hit
TGATCAGGGACCCTCCTCCTGTGATCCAGGAGGGCCAGATTCCCAGAGTGCCCTGGGGCTGGCC2810.1540401271790374No Hit
TGATCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2640.1447209735774586No Hit
TGATCAGGGAGTCTTTCTGAGAGAAACAGAGCTGTTAGGATTATATTGACTATAGGTCACTCTA2590.1419800460475825No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2520.13814274750575595No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2340.12827540839820195No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.12553448086832583No Hit
TGATCAGGGGCCCACTTCTCGCCTTGCGATAAGCCCAGGGTCTGCAACCTCATGGGCAGTGCTT2210.12114899682052406No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2080.11402258524284617No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.11237802872492052No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG2030.11128165771297008No Hit
TGATCAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1980.10854073018309396No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1930.10579980265321784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTA150.00460138258.2514558
TGACGGC258.421532E-658.2514558
ACCCTCG150.004606419758.2353655
CTAAATT150.004606419758.2353654
CGCTAGT201.9660768E-458.2353654
GTCACTC201.9660768E-458.2353656
TTGAACA201.9660768E-458.2353656
ATAAGCG150.004606419758.2353656
AATAAGC150.004606419758.2353655
ACCGAGT150.004611461458.21927650
CAATTAG150.004611461458.21927650
GTAGGAC150.004611461458.21927651
CGGTGAT150.004611461458.21927650
ACTTAAC150.004616507358.203247
TAGCCCA150.004616507358.203248
TTACAAT150.004616507358.203247
CGCCACA150.004616507358.203249
CCGATGC150.004626611258.1710845
TATATTG201.9795436E-458.15503742
AATTATC201.9795436E-458.15503739
Produced by FastQC (version 0.11.5)