FastQCFastQC Report
Fri 17 Jun 2016
SRR1526471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175071
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10250.5854767494330871No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.4175448817908163No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3290.1879237566473031No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2960.16907426129970127No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2810.16050630886897316No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.1479399786372386No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2570.1467975849798082No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.1290904832896368No Hit
TGATCAGGGACCCTCCTCCTGTGATCCAGGAGGGCCAGATTCCCAGAGTGCCCTGGGGCTGGCC2220.12680569597477595No Hit
TGATCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2210.12623449914606075No Hit
TGATCAGGGAGTCTTTCTGAGAGAAACAGAGCTGTTAGGATTATATTGACTATAGGTCACTCTA2040.11652415305790222No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2020.11538175940047181No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1900.10852739745588934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAT150.004616162758.2032833
TTTAAGG150.004621429458.18652735
CGTCAAG150.004637255358.13632648
CTCGTCA201.9824685E-458.13632250
TAAGGTG201.9824685E-458.13632249
GTAAGGT201.9824685E-458.13632248
CTAGTAC201.9852899E-458.11960656
CGCTAGT201.9852899E-458.11960654
CGGTCTA201.9852899E-458.11960653
ACACTAG201.9852899E-458.11960656
TATAGGT201.9881145E-458.10290551
AATGATC150.004658419658.0695243
CCGTATG201.9966076E-458.05284529
GATCCGT150.00466372258.0528429
CTGAACG150.00466372258.0528432
CTCCTAA150.00466372258.0528432
TATGAGT202.0051295E-458.0028728
GGCGTAT258.636363E-658.00286528
TAATGGG150.00469030157.96960414
TATTGTA202.0165369E-457.9363713
Produced by FastQC (version 0.11.5)