##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526471_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 175071 Sequences flagged as poor quality 0 Sequence length 64 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.30839487979163 39.0 38.0 40.0 35.0 40.0 2 37.81026554940567 39.0 38.0 40.0 33.0 40.0 3 37.58271215678211 39.0 38.0 40.0 33.0 40.0 4 37.969989318619305 39.0 38.0 40.0 35.0 40.0 5 38.136898743938175 39.0 38.0 40.0 35.0 40.0 6 37.95498968989724 39.0 38.0 40.0 35.0 40.0 7 38.25587904335955 39.0 38.0 40.0 35.0 40.0 8 38.10853310942418 39.0 38.0 40.0 35.0 40.0 9 38.26296188403562 39.0 38.0 40.0 35.0 40.0 10 37.508513688732 39.0 38.0 40.0 33.0 40.0 11 37.60643396107865 39.0 38.0 40.0 33.0 40.0 12 37.50124806507074 39.0 38.0 40.0 33.0 40.0 13 37.411884321218245 39.0 37.0 40.0 33.0 40.0 14 37.34323217437497 39.0 37.0 40.0 33.0 40.0 15 37.09463017861325 39.0 37.0 40.0 33.0 40.0 16 36.94895785138601 39.0 36.0 40.0 32.0 40.0 17 36.65855567169891 39.0 36.0 40.0 31.0 40.0 18 36.903593399249445 39.0 36.0 40.0 32.0 40.0 19 36.85625831805382 39.0 36.0 40.0 31.0 40.0 20 36.83033169399844 39.0 36.0 40.0 31.0 40.0 21 36.82684168137499 39.0 36.0 40.0 32.0 40.0 22 36.675274602875405 39.0 36.0 40.0 31.0 40.0 23 36.50235618691845 39.0 36.0 40.0 31.0 40.0 24 36.376470117837904 38.0 36.0 40.0 30.0 40.0 25 36.334030193464365 38.0 36.0 40.0 30.0 40.0 26 36.42150327581381 39.0 36.0 40.0 30.0 40.0 27 35.891586841909856 39.0 36.0 40.0 29.0 40.0 28 35.80712396684773 39.0 35.0 40.0 29.0 40.0 29 35.697979676816836 39.0 35.0 40.0 28.0 40.0 30 35.64089426575504 39.0 35.0 40.0 28.0 40.0 31 35.27516264829698 39.0 35.0 40.0 27.0 40.0 32 35.386700253040196 38.0 35.0 40.0 28.0 40.0 33 35.290739185816044 38.0 35.0 40.0 27.0 40.0 34 34.73592428214839 38.0 34.0 40.0 25.0 40.0 35 34.80953441746492 38.0 34.0 40.0 26.0 40.0 36 34.362510067344104 38.0 34.0 40.0 24.0 40.0 37 34.170776427849276 38.0 33.0 40.0 23.0 40.0 38 34.164813132957484 38.0 33.0 40.0 23.0 40.0 39 33.709889130695544 38.0 33.0 40.0 20.0 40.0 40 33.79036505189323 38.0 33.0 40.0 22.0 40.0 41 33.406218048677395 38.0 33.0 39.0 19.0 40.0 42 33.10191293817937 38.0 32.0 39.0 16.0 40.0 43 33.042354244849236 37.0 32.0 39.0 17.0 40.0 44 32.91213279183874 37.0 32.0 39.0 17.0 40.0 45 32.69688869087399 37.0 32.0 39.0 14.0 40.0 46 32.35737500785395 37.0 32.0 39.0 4.0 40.0 47 32.20123835472465 37.0 31.0 39.0 4.0 40.0 48 31.990306789816703 36.0 31.0 39.0 4.0 40.0 49 31.703091888433836 36.0 31.0 39.0 4.0 40.0 50 31.34254673818051 36.0 30.0 39.0 4.0 40.0 51 30.88886794500517 36.0 30.0 39.0 4.0 40.0 52 30.757167092208306 36.0 30.0 39.0 4.0 40.0 53 30.477623364235082 35.0 29.0 39.0 4.0 40.0 54 30.148402648068497 35.0 29.0 39.0 4.0 40.0 55 29.8391052772875 35.0 28.0 39.0 4.0 40.0 56 29.133522970680467 35.0 26.0 39.0 4.0 40.0 57 28.77707330168903 35.0 26.0 39.0 4.0 40.0 58 28.470643338988182 34.0 24.0 39.0 4.0 40.0 59 28.13925207487248 34.0 23.0 38.0 4.0 40.0 60 27.844011858046166 34.0 22.0 38.0 4.0 40.0 61 27.44525935192008 34.0 17.0 38.0 4.0 40.0 62 27.195840544693297 34.0 13.0 38.0 4.0 40.0 63 26.892129478897132 33.0 4.0 38.0 4.0 40.0 64 26.74902753739911 33.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.3450746891471823 2 2 -0.3112954230233598 2 3 -0.6084118289478866 2 4 0.2543210884990117 2 5 0.06293280564855053 2 6 0.18946035101563297 2 7 -0.0719833231541358 2 8 0.05877772264005188 2 9 0.010253203389126497 2 10 0.3752290685408255 2 11 -0.4136800197223849 2 12 0.2930493806046641 2 13 0.16338895635431783 2 14 -0.06510807104709215 2 15 0.1524022235101583 2 16 -0.11600470533279861 2 17 0.2280494084285678 2 18 0.3028938001884498 2 19 -0.7218132286616381 2 20 -0.20729551833764503 2 21 -0.6957278900639992 2 22 -0.8649481072744649 2 23 -0.9511865579375254 2 24 -0.8647735846648246 2 25 -0.6024263493689972 2 26 -0.6825454599192398 2 27 -0.05302763691029355 2 28 -0.24933820039736787 2 29 -0.2081772665144257 2 30 -0.7753629363922983 2 31 -0.18755137333297967 2 32 -0.10147177664706675 2 33 -0.6264670002643058 2 34 0.009905569696982752 2 35 -0.3570781666009495 2 36 -1.2858718140063843 2 37 -1.4486568586132194 2 38 -1.6552973288873503 2 39 -4.47479162643798 2 40 -1.602172979530181 2 41 -2.665496452967197 2 42 -2.2238917750059386 2 43 -2.8452264127609332 2 44 -1.7264250652824735 2 45 -2.2859838847810963 2 46 -0.8553917504501847 2 47 -1.5080310490849094 2 48 -1.3511324899307589 2 49 -1.5228424994320875 2 50 -1.799886552596579 2 51 -1.7438323517255476 2 52 -2.3021490890574086 2 53 -2.452279100486031 2 54 -2.1829187273616064 2 55 -3.511893659824885 2 56 -3.23193426386894 2 57 -3.4036185668889303 2 58 -3.382489646661835 2 59 -3.1329558604588534 2 60 -3.7597721100325927 2 61 -3.586373619886416 2 62 -3.8584042640189438 2 63 -3.310006877243733 2 64 -3.2619544026125737 3 1 -0.06346549374488575 3 2 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-1.733826270054891 119 58 -1.946917958821544 119 59 -2.0783077459196306 119 60 -1.7102661612525978 119 61 -0.9694145555589309 119 62 -1.3031511888021647 119 63 -0.937653580208039 119 64 -0.9908311922879136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 255.0 5 18.0 6 70.0 7 103.0 8 252.0 9 228.0 10 316.0 11 346.0 12 354.0 13 513.0 14 611.0 15 709.0 16 951.0 17 1033.0 18 1138.0 19 1215.0 20 1411.0 21 1568.0 22 1847.0 23 1794.0 24 2126.0 25 2380.0 26 2469.0 27 3023.0 28 3428.0 29 3841.0 30 4781.0 31 5685.0 32 6845.0 33 8246.0 34 10486.0 35 13017.0 36 16889.0 37 22363.0 38 28131.0 39 26519.0 40 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 100.0 0.0 0.0 7 99.46707031473126 0.36499685839949736 0.08053921288627407 0.08739361398297824 8 98.96499499928562 0.687526789541363 0.2114587798256894 0.13601943134733532 9 97.70664473270844 1.4040018049819787 0.3015919255616293 0.5877615367479481 10 50.927338051419135 33.05973005237875 5.837631589469415 10.175300306732698 11 30.34368913183794 28.039481124800798 20.312330425941475 21.30449931741979 12 36.939298913012436 17.17017667117912 25.78896561966288 20.101558796145564 13 24.894471385894864 17.21929959844863 31.766540432167524 26.119688583488987 14 28.628956252034886 21.44615613094116 27.547680655277002 22.37720696174695 15 27.83799853640688 22.449002927186243 26.835780278082694 22.877218258324188 16 27.128549963721955 21.339488222490104 27.359929614881423 24.172032198906518 17 30.028827674567587 20.52999450901437 21.440582959641254 28.00059485677679 18 28.095458414014885 25.12809088883938 22.70107556362847 24.07537513351726 19 28.616389921803155 22.226410998966134 24.54375653306373 24.613442546166983 20 27.33100233100233 23.551122080533844 22.651857945975593 26.466017642488232 21 28.866002935951702 22.05276716303671 25.06583043450943 24.015399466502164 22 27.733319624609443 23.80005826207653 24.505486345539808 23.961135767774216 23 30.503624243878196 23.987410821895118 23.741224988718862 21.76773994550782 24 26.233562966263378 27.46569786704139 22.52344883526979 23.777290331425437 25 26.523672359006014 22.741352902178637 25.41949569073909 25.315479048076263 26 27.219073519665425 22.30614530246589 23.26941974998286 27.205361427885823 27 24.641961740563932 21.422765623747868 25.10732561733695 28.827947018351253 28 25.39723409490133 23.272739756595772 25.58948566981936 25.74054047868353 29 26.8561037119215 23.2665595388548 25.591156808811167 24.286179940412538 30 25.3458331187079 24.420368240014195 27.308940437149086 22.92485820412882 31 26.27758536794802 24.061596588144376 28.471248246844322 21.189569797063285 32 26.29790253000881 23.79821720772162 29.750203110160083 20.153677152109484 33 26.711897400664146 23.927058284667353 29.537959464101682 19.823084850566815 34 24.368788957892505 25.569566538790163 31.56427944743588 18.49736505588146 35 22.951711655984923 28.196360762212407 30.578990475208624 18.272937106594043 36 23.569807167587644 26.857244399605623 29.722215853071333 19.8507325797354 37 22.876146788990827 27.80619266055046 30.002293577981654 19.31536697247706 38 24.36727183091325 26.741686396129396 30.651272908629178 18.239768864328177 39 23.703840026685224 25.812202738686096 30.099091897238882 20.384865337389794 40 23.142111751383272 25.422436283363435 31.408503196582664 20.026948768670625 41 21.348340389403276 26.451083651716978 31.4907238495164 20.709852109363347 42 21.298013701383343 24.87725604819028 31.338038278885744 22.486691971540633 43 20.94306366512226 24.89077203075033 33.430361069486786 20.735803234640624 44 21.49346587159017 24.342426207680255 32.81641882701347 21.347689093716102 45 21.476675757140807 25.431893210851154 31.633127601550164 21.458303430457875 46 21.020596831521708 26.210860565135253 31.988538813571978 20.78000378977106 47 21.208728107952915 26.265288544358313 32.22911283376399 20.296870513924777 48 22.761305436255483 26.617441032589973 31.457132358007396 19.16412117314715 49 22.004961582193435 26.78507850095901 31.566343934121218 19.64361598272634 50 20.728924147049526 26.42235060791584 31.77028541864275 21.078439826391886 51 19.677376732750055 28.419111522177477 31.300661216845803 20.602850528226664 52 20.842932568841597 28.301323114576093 30.555156584836173 20.30058773174613 53 21.455786769649844 27.177329908223136 30.731169077461512 20.635714244665508 54 21.133801441097717 27.0786168220003 30.79915418826206 20.98842754863992 55 22.113709913835223 27.52386919508648 29.329938092418768 21.032482798659533 56 21.29129802413351 28.215741395466488 29.16429528195046 21.328665298449543 57 22.215899292981547 28.228430189113062 28.870494912916023 20.685175604989368 58 21.717348916663312 29.819223402603228 28.353684840188425 20.109742840545035 59 21.916649889269177 30.508786562742674 28.515631740918636 19.058931807069516 60 22.775763852925945 29.298578744461707 27.345647045284537 20.58001035732781 61 22.25422393920057 28.54744781516422 28.390505263036868 20.80782298259835 62 21.391607346159596 28.173695044677256 27.890796600618696 22.543901008544452 63 22.067736185383243 29.003507561382325 26.98062216088782 21.948134092346617 64 21.862697700117412 28.779036305453875 27.12031217625527 22.237953818173448 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 9.0 6 10.0 7 163.5 8 317.0 9 331.5 10 327.0 11 308.0 12 339.0 13 545.5 14 721.0 15 948.5 16 1289.5 17 1403.0 18 1640.5 19 1878.0 20 2176.0 21 2976.0 22 3478.0 23 3562.5 24 4102.5 25 4558.0 26 4840.5 27 4400.5 28 3678.0 29 3765.5 30 3545.0 31 3237.0 32 3302.0 33 3367.0 34 3432.0 35 3416.0 36 3335.0 37 3359.0 38 3546.0 39 3709.0 40 3548.5 41 3505.0 42 3622.0 43 3920.0 44 4218.0 45 4157.0 46 4496.0 47 4896.0 48 4977.5 49 5082.0 50 5105.0 51 5304.5 52 6422.5 53 7341.0 54 7890.0 55 8469.5 56 8500.0 57 9209.5 58 9919.0 59 9790.0 60 9683.0 61 9705.0 62 8617.0 63 6871.5 64 6214.0 65 5818.0 66 4495.0 67 3568.0 68 3180.5 69 2793.0 70 2416.0 71 1911.5 72 1784.0 73 1370.0 74 784.0 75 612.0 76 469.5 77 274.5 78 222.0 79 178.0 80 109.0 81 84.0 82 58.0 83 32.0 84 23.0 85 9.0 86 4.0 87 4.0 88 2.0 89 0.0 90 1.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 5.71196828715207E-4 8 0.054834895556659866 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.09082029576571791 16 0.019420692176317037 17 0.13651604206293447 18 0.0 19 0.0 20 0.022276676319893073 21 0.0 22 0.0 23 0.0 24 0.005140771458436863 25 0.05654848604280548 26 0.0245614636347539 27 0.21077162979591138 28 0.1713590486145621 29 0.11538175940047181 30 0.19877649639289202 31 0.22048197588406987 32 0.16507588349869481 33 0.23476189660195007 34 0.3906986308412016 35 0.2707472968110081 36 0.35357083697471314 37 0.3832730720679039 38 0.35871160843315 39 0.6808666198285267 40 0.3804170879243278 41 0.43011121202255087 42 0.5300706570477121 43 0.510649964871395 44 0.47523576149105223 45 0.5117923585288254 46 0.52435868876056 47 0.5260722792467056 48 0.5180755236446928 49 0.5329266411912882 50 0.637455660846171 51 0.5700544350577765 52 0.5780511906597894 53 0.6066110320955498 54 0.5923311113776696 55 0.6294589052441582 56 0.6408828418184622 57 0.628887708415443 58 0.6911481627454005 59 0.7002873120048437 60 0.7317031375841802 61 0.6408828418184622 62 0.6608747308234945 63 0.6631595181383553 64 0.7556934043902188 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 175071.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.409925116095756 #Duplication Level Percentage of deduplicated Percentage of total 1 79.90887713109936 43.478360208144124 2 8.437263794406652 9.181417824768237 3 3.8538254808096077 6.290590674640574 4 2.176240866717057 4.736364103706496 5 1.4266817838246408 3.8812824511198314 6 0.916477702191988 2.9919289888102543 7 0.668724279835391 2.546966659241108 8 0.5028554631729235 2.188826247636673 9 0.3516838834299152 1.7221584385763489 >10 1.6313932980599646 15.301220647622966 >50 0.0818846056941295 3.0530470494827813 >100 0.04199210548416898 3.6248150750267034 >500 0.0010498026371042243 0.4175448817908163 >1k 0.0010498026371042243 0.5854767494330871 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1025 0.5854767494330871 No Hit TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 731 0.4175448817908163 No Hit TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 329 0.1879237566473031 No Hit TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 296 0.16907426129970127 No Hit TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 281 0.16050630886897316 No Hit TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 259 0.1479399786372386 No Hit TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 257 0.1467975849798082 No Hit TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.1290904832896368 No Hit TGATCAGGGACCCTCCTCCTGTGATCCAGGAGGGCCAGATTCCCAGAGTGCCCTGGGGCTGGCC 222 0.12680569597477595 No Hit TGATCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 221 0.12623449914606075 No Hit TGATCAGGGAGTCTTTCTGAGAGAAACAGAGCTGTTAGGATTATATTGACTATAGGTCACTCTA 204 0.11652415305790222 No Hit TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 202 0.11538175940047181 No Hit TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 190 0.10852739745588934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.01142393657430414 0.0 0.0 0.0 0.0 7 0.01142393657430414 0.0 0.0 0.0 0.0 8 0.011995133403019347 0.0 0.0 0.0 0.0 9 0.013708723889164968 0.0 0.0 0.0 0.0 10 0.025703857292184315 0.0 0.0 0.0 0.0 11 0.0325582192367668 0.0 0.0 0.0 0.0 12 0.033129416065482004 0.0 0.0 0.0 0.0 13 0.03541420338034283 0.0 0.0 0.0 0.0 14 0.0491229272695078 0.0 0.0 0.0 0.0 15 0.05312130507051425 0.0 0.0 0.0 0.0 16 0.05654848604280549 0.0 0.0 0.0 0.0 17 0.060546863843811936 0.0 0.0 0.0 0.0 18 0.0651164384735336 0.0 0.0 0.0 0.0 19 0.06854361944582484 0.0 0.0 0.0 0.0 20 0.07596917821912252 0.0 0.0 0.0 0.0 21 0.08110994967755938 0.0 0.0 0.0 0.0 22 0.10452901965488287 0.0 0.0 0.0 0.0 23 0.1148105625717566 0.0 0.0 0.0 0.0 24 0.11938013720147826 0.0 0.0 0.0 0.0 25 0.12623449914606075 0.0 0.0 0.0 0.0 26 0.1302328769470672 0.0 0.0 0.0 0.0 27 0.13880082937779528 0.0 0.0 0.0 0.0 28 0.1547943405818211 0.0 0.0 0.0 0.0 29 0.16050630886897316 0.0 0.0 0.0 0.0 30 0.17021665495713167 0.0 0.0 0.0 0.0 31 0.17821341055914458 0.0 0.0 0.0 0.0 32 0.18278298518886624 0.0 0.0 0.0 0.0 33 0.1890661503047335 0.0 0.0 0.0 0.0 34 0.19420692176317036 0.0 0.0 0.0 0.0 35 0.2210531727127851 0.0 0.0 0.0 0.0 36 0.22847873148608278 0.0 0.0 0.0 0.0 37 0.23419069977323487 0.0 0.0 0.0 0.0 38 0.245614636347539 0.0 0.0 0.0 0.0 39 0.2541825887782671 0.0 0.0 0.0 0.0 40 0.2696049031535777 0.0 0.0 0.0 0.0 41 0.28274243021402745 0.0 0.0 0.0 0.0 42 0.28959679215860995 0.0 0.0 0.0 0.0 43 0.3033055160477749 0.0 0.0 0.0 0.0 44 0.3158718462795095 0.0 0.0 0.0 0.0 45 0.3290093733399592 0.0 0.0 0.0 0.0 46 0.34043330991426335 0.0 0.0 0.0 0.0 47 0.35357083697471314 0.0 0.0 0.0 0.0 48 0.36499477354901727 0.0 0.0 0.0 0.0 49 0.3787034974381822 0.0 0.0 0.0 0.0 50 0.3849866625540495 0.0 0.0 0.0 0.0 51 0.39526820547092323 0.0 0.0 0.0 0.0 52 0.40554974838779695 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGAAT 15 0.0046161627 58.20328 33 TTTAAGG 15 0.0046214294 58.186527 35 CGTCAAG 15 0.0046372553 58.136326 48 CTCGTCA 20 1.9824685E-4 58.136322 50 TAAGGTG 20 1.9824685E-4 58.136322 49 GTAAGGT 20 1.9824685E-4 58.136322 48 CTAGTAC 20 1.9852899E-4 58.119606 56 CGCTAGT 20 1.9852899E-4 58.119606 54 CGGTCTA 20 1.9852899E-4 58.119606 53 ACACTAG 20 1.9852899E-4 58.119606 56 TATAGGT 20 1.9881145E-4 58.102905 51 AATGATC 15 0.0046584196 58.06952 43 CCGTATG 20 1.9966076E-4 58.052845 29 GATCCGT 15 0.004663722 58.05284 29 CTGAACG 15 0.004663722 58.05284 32 CTCCTAA 15 0.004663722 58.05284 32 TATGAGT 20 2.0051295E-4 58.00287 28 GGCGTAT 25 8.636363E-6 58.002865 28 TAATGGG 15 0.004690301 57.969604 14 TATTGTA 20 2.0165369E-4 57.93637 13 >>END_MODULE