FastQCFastQC Report
Fri 17 Jun 2016
SRR1526470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160915
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA14390.8942609452195258No Hit
TGATCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9390.5835378926762577No Hit
TGATCAGGGGCCCACTTCTCGCCTTGCGATAAGCCCAGGGTCTGCAACCTCATGGG3930.2442283192990088No Hit
TGATCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.22682782835658577No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3550.2206133673057204No Hit
TGATCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3290.20445576857347045No Hit
TGATCAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3010.18705527763104743No Hit
TGATCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2620.16281887953267252No Hit
TGATCAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2580.16033309511232638No Hit
TGATCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2570.15971164900723983No Hit
TGATCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2340.14541838859024953No Hit
TGATCAGGGAGTCTTTCTGAGAGAAACAGAGCTGTTAGGATTATATTGACTATAGG2280.1416897119597303No Hit
TGATCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.136718143119038No Hit
TGATCAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2150.13361091259360533No Hit
TGATCAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2130.13236802038343226No Hit
TGATCAGGGACCCTCCTCCTGTGATCCAGGAGGGCCAGATTCCCAGAGTGCCCTGG2100.13050368206817264No Hit
TGATCAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1870.1162104216511823No Hit
TGATCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.10751017617997079No Hit
TGATCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG1700.10564583786471118No Hit
TGATCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC1670.10378149954945158No Hit
TGATCAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1660.10316005344436503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG150.00799960650.6485137
TGATGGT203.9157865E-450.6485137
TGGGCAT203.9219126E-450.6324835
ACCTGTA150.00800962850.63247735
CCAGATT354.755566E-850.63247735
TTATCCT150.00800962850.63247736
AAAGCGA150.00806995850.5364933
ACGTGTC251.9630214E-550.48863631
CGTTTCG204.058625E-450.282348
TAGTCCT150.00823248950.282348
ATCTCCC150.008252975550.25070644
CGAAATG150.008252975550.25070646
ATAATAG150.008252975550.25070646
TCGGGAC150.008252975550.25070645
TATTAAC204.0712385E-450.25070646
TCATAAT150.008252975550.25070644
GTGAATC204.0712385E-450.25070643
GCCCGAT150.008252975550.25070647
AACACGG150.00826323350.23492442
CTATAGG204.0965594E-450.18763750
Produced by FastQC (version 0.11.5)