FastQCFastQC Report
Fri 17 Jun 2016
SRR1526469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116922
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG22321.9089649509929696No Hit
GCGTCCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC17491.495869040899061No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14071.2033663467952993No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6360.5439523785087494No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4410.3771745266074819No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3860.3301346196609706No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3620.3096081148115838No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.2865157968560237No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2720.2326337216263834No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC2690.23006790852021006Illumina Single End Adapter 2 (96% over 31bp)
GCGTCCGGGGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG2610.2232257402370811No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2550.21809411402473447No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA2540.21723884298934332No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA2430.20783086160004105No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA2010.1719094781136142No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1860.15908041258274747No Hit
GCGTCCGGGACCCAGCCGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAG1850.15822514154735637No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.15309351533500967No Hit
GCGTCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1670.14283026291031628No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.1394091787687518No Hit
GCGTCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1530.13085646841484067No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.1265801132378851No Hit
GCGTCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1400.11973794495475616No Hit
GCGTCCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.11204050563623613No Hit
GCGTCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC1300.11118523460084502No Hit
GCGTCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1280.10947469253006277No Hit
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1240.10605360838849831No Hit
GCGTCCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.10434306631771609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT201.9838862E-458.11168738
CACATTA201.9838862E-458.11168740
ACATTAT201.9838862E-458.11168741
CAACCAA201.9838862E-458.11168749
ATTAAGC850.058.11168751
CATTATT201.9838862E-458.11168742
TTTCACT201.9838862E-458.11168747
GCATTAA201.9838862E-458.11168739
TTCTAGG150.004641194458.11168340
CCCCTTG150.004641194458.11168348
ATAGGGA150.004641194458.11168345
AACCCAC150.004641194458.11168352
TTTAATC150.004641194458.11168338
CGGTTGG150.004641194458.11168354
ATCAGGC150.004641194458.11168343
AGCTTAT150.004641194458.11168354
GATCCCG150.004641194458.11168353
ATGCTTA150.004641194458.11168353
TATATGC150.004641194458.11168350
AGGGTTG150.004641194458.11168349
Produced by FastQC (version 0.11.5)