FastQCFastQC Report
Fri 17 Jun 2016
SRR1526468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113146
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG18511.6359394057235785No Hit
GCGTCCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC13571.1993353719972426No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11501.0163859084722395No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4980.44013928906015237No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.29872907570749296No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3140.2775175437045941No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.24746787336715395No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.2457002457002457No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2680.23686210736570448No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2140.18913616035918193No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC2140.18913616035918193Illumina Single End Adapter 2 (96% over 31bp)
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA1990.17587895285737012No Hit
GCGTCCGGGGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG1960.17322751135700776No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA1860.16438937302246653No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1490.13168826118466406No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA1460.1290368196843017No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.12285012285012285No Hit
GCGTCCGGGACCCAGCCGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAG1370.1210824951832146No Hit
GCGTCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1280.11312817068212752No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.10429003234758631No Hit
GCGTCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1140.10075477701376981No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGG150.004615947658.19047549
TTGTGCG201.974688E-458.16459758
TGTTGTG201.974688E-458.16459756
GATCTTT201.974688E-458.16459754
TTAGATG150.00462409158.16459356
GTGCTTA150.00462409158.16459358
TAGATGA150.00462409158.16459357
AGGTGCT150.00462409158.16459356
TTGGCAT201.9790376E-458.13873336
TAGATTA150.004632245358.13873352
ATTAAGC950.058.13872551
AACCGCT150.004640409758.11289231
TGAGTTA201.9833948E-458.11289246
AGGGTCC150.004640409758.11289244
AAAGTCC150.004640409758.11289231
GAACCGC150.004640409758.11289230
TGTGTAT201.9833948E-458.11289246
GATAGGT201.9833948E-458.11289244
TAGACGA201.9877596E-458.0870829
CAATGAA201.9877596E-458.0870829
Produced by FastQC (version 0.11.5)