FastQCFastQC Report
Fri 17 Jun 2016
SRR1526467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106232
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA18361.728292793132013No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT15841.4910761352511486No Hit
GCGTCCGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG13891.30751562617667No Hit
GCGTCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6300.5930416447021614No Hit
GCGTCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.4339558701709466No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3390.3191128850064011No Hit
GCGTCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.27769410347164697No Hit
GCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.2560433767602982No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2150.20238722795391217No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2020.1901498606822803No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA1940.18261917313050682No Hit
GCGTCCGGGGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAAC1920.18073650124256346No Hit
GCGTCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1910.17979516529859177No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAA1910.17979516529859177No Hit
GCGTCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1810.1703818058588749No Hit
GCGTCCGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA1720.16190978236312975No Hit
GCGTCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.14590707131561112No Hit
GCGTCCGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1520.14308306348369607No Hit
GCGTCCGGGACCCAGCCGGGCAGTGGTGGCGCACGCCTTTAATCCCAGCACTTGGG1490.140259055651781No Hit
GCGTCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG1450.13649371187589426Illumina Single End Adapter 2 (95% over 23bp)
GCGTCCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1360.1280216883801491No Hit
GCGTCCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.12237367271631899No Hit
GCGTCCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1290.12143233677234733No Hit
GCGTCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1280.12049100082837563No Hit
GCGTCCGGGGAGTGCAGTGCTGCGTCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA1270.11954966488440394No Hit
GCGTCCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1240.1167256570524889No Hit
GCGTCCGGGACCTCAGATTATTTTTAAAAAATAAGCCAATAACAAGTTTTGAAGGC1240.1167256570524889No Hit
GCGTCCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1170.1101363054446871No Hit
GCGTCCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC1130.10637096166880035No Hit
GCGTCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1120.10542962572482868No Hit
GCGTCCGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1070.10072294600497024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC150.00801013750.6195537
TGCGATT150.00804054350.57108736
ATACAGG150.00804054350.57108736
GCCTTTA203.9579323E-450.52271735
ATATGCT402.410161E-950.52271734
TTGCGAT150.00807103550.52271735
AGGGCGC150.00807103550.52271735
AACAGAA203.9579323E-450.52271735
CATATGC402.4610927E-950.40219533
GTTAAAG204.0048955E-450.40219532
TGCGATC204.0048955E-450.40219532
TTAAAGG150.00814764350.40219533
GTGACAC150.00814764350.40219532
ATGGAAC204.0143408E-450.3781631
GTGCGAT204.0143408E-450.3781631
GGCCTAA251.9839756E-550.3781631
ATTCGTT150.00820931750.3061948
TCAGGGT204.0427837E-450.30618747
AAGATTA402.5029294E-950.30618748
GCACTTG204.0427837E-450.30618748