FastQCFastQC Report
Fri 17 Jun 2016
SRR1526466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921476
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC45670.4956178999778616No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC33490.36343865711098283No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC32130.3486797268729734No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC27180.29496156166845366No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC25590.2777066358754867No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.27065273539408513No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15650.16983621928297643No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT13720.14889156093050715No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT13380.1452018283710048No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC12530.13597749697224887No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC12380.13434967378423313No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC12010.1303343765871276No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCAAAAAAAAA11930.12946620422018587No Hit
GCCTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG10380.11264536461068982No Hit
GCCTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9610.10428920557887564No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA9280.10070799456524099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGT351.6063495E-858.1561252
TATACGG150.00463731658.15611655
TCGATAC258.714867E-657.947525
TAGGGGG521050.054.9244884
CTAGGGG928000.054.533623
GCCTAGG971800.054.4747351
CCTAGGG962650.054.4697042
TAGGGGA292650.053.8866044
AGGGGGG243900.053.734825
TAGGGGT53350.053.349974
AGGGGAT71400.053.0696955
AGGGGGA117150.053.035365
CGGTACT553.6379788E-1252.86631851
AGGGGAC53900.052.8592535
TAGGGGC64250.052.4927064
AGGGGAG95550.052.2194025
AGGGGGC110100.052.0965425
ACGGTAC451.9972504E-951.6915150
AGGGGGT69500.051.2742655
CTAGGGT8500.050.3635443
Produced by FastQC (version 0.11.5)