FastQCFastQC Report
Fri 17 Jun 2016
SRR1526465_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526465_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892751
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC38370.4297950940407796No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC27600.30915675255474373No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC27130.3038921266960216No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC22730.25460626759309146No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC21850.24474909577250542No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20230.22660293855733568No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT15120.1693641340082509No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT12330.13811241880434746No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12070.13520007258462885No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC11020.12343867438961142No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC10270.11503767567888472No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCAAAAAAAAA9560.10708473023273007No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC9440.1057405704390138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTC258.593999E-658.0839430
TAGGGGG504400.054.6649364
CTAGGGG895900.054.4729583
GCCTAGG939450.054.432521
CCTAGGG928750.054.4248962
AGGGGAT70000.054.390795
TAGGGGA279500.053.8369674
TAGGGGT51300.053.6077544
AGGGGGG239050.053.291495
AGGGGGC108950.053.1347275
AGGGGAG90100.053.0296445
AGGGGGA107550.052.993755
TAGGGGC65000.052.7972264
AGGGGAC49950.051.297495
AGGGGGT66200.050.5310825
AGGGGTG27600.049.6109165
CTAGGGA20000.049.2529643
AGGGGAA79650.048.9616435
CTAGGGT8750.048.530783
AGGGGTA11200.047.9736675
Produced by FastQC (version 0.11.5)