FastQCFastQC Report
Fri 17 Jun 2016
SRR1526464_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526464_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840051
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC94271.122193771568631No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC41740.49687459451866617No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC35080.4175936937162148No Hit
GCCTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT26680.31759976477618623No Hit
GCCTAGGGAGCCCTCCCTACTCTCTTGAATACCATCAATAAAGTTCGCTGCACCCA18310.21796295701094337No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC17220.2049875543270587No Hit
GCCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT15240.18141755679119484No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC14950.17796538543493193No Hit
GCCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC13280.15808564003852146No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT12310.14653872205378007No Hit
GCCTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12210.14534831813782734No Hit
GCCTAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA12160.14475311617985098No Hit
GCCTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA11030.13130155192958523No Hit
GCCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC10380.12356392647589252No Hit
GCCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC10270.12225448216834453No Hit
GCCTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC9540.11356453358188968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.00828181250.22514347
AATTCGA150.00829953850.1979849
TGCGATA252.1137968E-549.89220425
TACGCGA204.2982382E-449.7259118
GGTATAG150.00861534649.7259118
TAGGGGG469700.047.25064
CTAGGGG844050.046.961063
GCCTAGG883350.046.8913041
CCTAGGG875350.046.8878062
TAGGGGA266400.046.5486264
AGGGGAT63700.046.1685
AGGGGGG216950.046.068625
AGGGGAG88250.046.0399135
AGGGGGA108100.045.963745
AGGGGAC49800.045.6146625
TAGGGGT48400.045.4947134
TAGGGGC62800.045.244894
AGGGGGC99550.045.0877235
AGGGGGT61750.044.4025235
CTAGGGT7250.043.5841673
Produced by FastQC (version 0.11.5)