FastQCFastQC Report
Fri 17 Jun 2016
SRR1526463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196752
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.6891924859721884No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC12960.6586972432300561No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC9600.48792388387411567No Hit
TTCATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6210.31562576238106854No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.3125762381068553No Hit
TTCATAGGGGGGGTTGGAGAGATGGCTCAGCGGGTAAGAACACTGACTGCTCTTCCAGGGGTCC4640.23582987720582255No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA4440.22566479629177846No Hit
TTCATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4140.21041717492071235No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3220.1636578027161096No Hit
TTCATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3050.15501748393917217No Hit
TTCATAGGGTGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGT3020.15349272180206555No Hit
TTCATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.13926160852240382No Hit
TTCATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2670.1357038302024884No Hit
TTCATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2410.12248922501423111No Hit
TTCATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.1209644628771245No Hit
TTCATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.1209644628771245No Hit
TTCATAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.11740668455720907No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAATATAAG2290.11639017646580467No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC2230.11334065219159144No Hit
TTCATAGGGTGATGTAGGGGGAAGAGCACCAGCCTCAGAACCTTGTGGTGTCAGCATCAGCATC2150.10927461982597382No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2080.10571684150605838No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCT2030.10317557127754737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTTG150.004619668258.19488557
TACAGTA150.004619668258.19488555
TTACCTG201.9732382E-458.19488553
CTTACAG150.004619668258.19488553
AGACTAA351.5956175E-858.1651348
TGATAAA150.00462903458.1651348
ACCCTCG258.498433E-658.1651351
CGTCATC150.00462903458.1651351
TGCACGT150.00462903458.1651351
GCCTCTA258.52423E-658.13541439
CCTTAGT150.004638413458.1354144
GTCGCGT201.9832414E-458.1354144
TGAAGCG150.004638413458.1354143
ATTGCTG150.004638413458.1354143
CGGTAAG150.004638413458.1354141
TCGCGTG201.9832414E-458.1354145
AGTAAGG150.004638413458.1354142
TATGAGT201.9882582E-458.10572436
TTCGGAT258.550094E-658.1057237
AATCCAT150.004652509558.0908935
Produced by FastQC (version 0.11.5)