FastQCFastQC Report
Fri 17 Jun 2016
SRR1526462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189169
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.5978780878473745No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC10510.5555878605902658No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC7660.404928925986816No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.25638450274622165No Hit
TTCATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.25004096865765535No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3600.19030602265698923No Hit
TTCATAGGGGGGGTTGGAGAGATGGCTCAGCGGGTAAGAACACTGACTGCTCTTCCAGGGGTCC3570.18872013913484767No Hit
TTCATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG3320.1755044431170012No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2590.1369146107448895No Hit
TTCATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2540.13427147154132021No Hit
TTCATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2330.12317028688632915No Hit
TTCATAGGGTGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGT2230.11788400847919057No Hit
TTCATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2080.10995459086848268No Hit
TTCATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.10889733518705497No Hit
TTCATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.10784007950562725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC150.004598207358.26241337
GACTACA150.004598207358.26241339
TTCTATG150.004603070758.2468635
TAAGACT150.004603070758.2468635
TAGCCAG258.493524E-658.16924749
ACGCTTG258.493524E-658.16924757
AATTCGT303.669993E-758.1692449
ACTAAAT150.004627445258.1692448
CACGTGG150.004627445258.1692453
GAGATTG303.669993E-758.1692457
TAGCCGC150.004632331458.1537447
CGACGGC150.00463722258.13825244
TACACTT150.00463722258.13825245
TATCCTA150.00463722258.13825252
CGCGATT150.00464211658.1227730
CAATTCC150.00464211658.1227730
TTATCCT150.004647013758.10729251
TACTGCG150.004647013758.10729232
ATTAGCG258.57439E-658.07637429
CGTGCGG150.004656821558.07636642
Produced by FastQC (version 0.11.5)