FastQCFastQC Report
Fri 17 Jun 2016
SRR1526461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169368
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14040.8289641490718435No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC13010.7681498275943508No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12300.7262292758962732No Hit
TTCATAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6200.3660667894761703No Hit
TTCATAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.3412687166406877No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5590.3300505408341599No Hit
TTCATAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4410.26037976477256625No Hit
TTCATAGGGGGGGTTGGAGAGATGGCTCAGCGGGTAAGAACACTGACTGCTCTTCC3840.22672523735298286No Hit
TTCATAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG3120.18421425534929858No Hit
TTCATAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.16827263709791696No Hit
TTCATAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.16413962495866988No Hit
TTCATAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG2720.1605970431250295No Hit
TTCATAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2680.15823532190260262No Hit
TTCATAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2380.14052241273440083No Hit
TTCATAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2280.13461810967833357No Hit
TTCATAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.13284681876151339No Hit
TTCATAGGGTGTCATTACCTTTATGTTACTTTATGCTGCTGGCTCTTGTACCTTGG2190.12930423692787302No Hit
TTCATAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCA2170.12812337631665957No Hit
TTCATAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.11867649142695195No Hit
TTCATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC1880.11100089745406452No Hit
TTCATAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1870.11041046714845779No Hit
TTCATAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC1870.11041046714845779No Hit
TTCATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1800.10627745500921071No Hit
TTCATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.10214444286996363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT150.00803413450.59460437
CTCGTCC150.00803413450.59460437
GCGGTTT150.00803413450.59460437
CCACTTA150.00803413450.59460437
CAGTACG550.050.59460437
TAAATAC203.9370576E-450.594637
GGCATAG150.00805320450.5642834
ACTCCAC150.00805320450.5642834
GTCGCTA150.00812021350.45843533
GTATTGT150.00812981950.4433532
TATGCGT150.00812981950.4433532
CGTCGCT150.00812981950.4433532
CAGGTCG150.00813943450.42827631
ACGAGGT150.00813943450.42827631
TTGATCA301.0284039E-650.09887350
CTCGTGG252.0556614E-550.09887338
AGTACGT600.050.09887338
CTAATCG150.00835311150.09887349
GTACGTA600.050.09887339
CCTCTAC150.00835311150.09887340
Produced by FastQC (version 0.11.5)