FastQCFastQC Report
Fri 17 Jun 2016
SRR1526460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95461
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.8673699207006003No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5100.5342495888373262No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4280.44835063533799147No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3310.3467384586375588No Hit
CTCCATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC2760.28912330690020005No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.28179046940635444No Hit
CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2380.24931647479075222No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1900.1990341605472392No Hit
CTCCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1850.19379641948020657No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1800.18855867841317397No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.1812258409193283No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1700.17808319627910874No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1680.1759880998522957No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1660.17389300342548264No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGACC1640.1717979069986696No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1630.1707503587852631No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC1600.16760771414504352No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.16446506950482395No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1560.16341752129141746No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1550.1623699730780109No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1530.16027487665119786No Hit
CTCCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1530.16027487665119786No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1520.15922732843779136No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1500.1571322320109783No Hit
CTCCATGGGTACTATTATTTGTTTTCAATGTATAAAGGCAGTATTTCCCTTTTCAGGTTGCTGA1500.1571322320109783No Hit
CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1480.15503713558416526No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1460.15294203915735222No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC1380.14456165345010002No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT1360.14246655702328698No Hit
CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1300.13618126774284786No Hit
CTCCATGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTTC1280.1340861713160348No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1270.1330386231026283No Hit
CTCCATGGGAACACCGGAAATGGAATCAAGTGACCTAAACACTTGACTCCACACTCTCTTAGCT1240.12989597846240875No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1220.1278008820355957No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1190.12465823739537611No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACGC1160.12151559275515655No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1160.12151559275515655No Hit
CTCCATGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC1150.12046804454175003No Hit
CTCCATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.11942049632834352No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC1070.11208765883449787No Hit
CTCCATGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG1020.10684991776746525No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC990.10370727312724569No Hit
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC970.10161217670043264Illumina Single End Adapter 2 (96% over 31bp)
CTCCATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC960.1005646284870261No Hit
CTCCATGGGGCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGG960.1005646284870261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCAT201.9709404E-458.17755558
GAGACAC201.9709404E-458.17755554
ATGAGTC201.9709404E-458.17755557
AGACACG201.9709404E-458.17755555
AAAGAGC501.8189894E-1258.17755558
ACGCGCT201.9709404E-458.17755558
GTAATGA150.004617858.1775554
CCTCCGA150.004617858.1775557
ATGACAC150.004617858.1775552
TAATGAG150.004617858.1775555
TCGAATG150.004617858.1775548
AGTAATG150.004617858.1775553
GTACAAA150.00462744758.14691547
ACTTGAA150.00462744758.14691544
CTTATAT150.00462744758.14691545
GCTTATA150.00462744758.14691544
CTCGAAT150.00462744758.14691547
AACCCTT150.00463710958.11631439
GTTGTAG150.00463710958.11631440
AGACAGG201.9864031E-458.08574337
Produced by FastQC (version 0.11.5)