FastQCFastQC Report
Fri 17 Jun 2016
SRR1526459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92812
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.6734043011679524No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4580.4934706718958755No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3830.4126621557557213No Hit
CTCCATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC2680.28875576434081796No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2640.2844459768133431No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.24565788906606903No Hit
CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.20579235443692626No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1440.15515235098909624No Hit
CTCCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1430.15407490410722752No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.15084256346162134No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1390.1497651165797526No Hit
CTCCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1360.14653277593414646No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1340.14437788217040898No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1310.14114554152480283No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1310.14114554152480283No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGACC1300.1400680946429341No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.13683575399732795No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1260.13575830711545922No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1240.13360341335172174No Hit
CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1230.13252596646985304No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC1220.1314485195879843No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1180.12713873206050944No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1160.12498383829677197No Hit
CTCCATGGGTACTATTATTTGTTTTCAATGTATAAAGGCAGTATTTCCCTTTTCAGGTTGCTGA1160.12498383829677197No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1150.12390639141490324No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1140.12282894453303451No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC1110.11959660388742835No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1110.11959660388742835No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1110.11959660388742835No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC1110.11959660388742835No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT1070.11528681635995346No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1040.11205447571434729No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1000.10774468818687238No Hit
CTCCATGGGAACACCGGAAATGGAATCAAGTGACCTAAACACTTGACTCCACACTCTCTTAGCT990.10666724130500367No Hit
CTCCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC980.10558979442313494No Hit
CTCCATGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTGCCTGGCTC950.10235745377752876No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA950.10235745377752876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG201.9728833E-458.1642333
ACTTTGA201.9728833E-458.1642355
ACTATAT150.004621580758.16422733
CTATATC150.004621580758.16422734
ACCTAAA150.004621580758.16422733
ACCCAGG150.004621580758.16422755
GGCCGAA258.463796E-658.16422333
ATATCTC201.9781801E-458.1327236
AGCTCTA150.004631513758.1327253
TATATCT201.9834883E-458.10124635
AGGTTCG201.9834883E-458.10124652
ATTGGCC150.00464146258.10124635
ACACGGT150.004651426358.06980528
TTGTGCA150.004651426358.06980551
CGCGCTT150.004651426358.06980530
TTCACAG150.004651426358.06980551
ATACTTT201.9888078E-458.06980549
AACTATC201.9888078E-458.06980549
GTGCGAT201.9888078E-458.06980531
CTAGCAT150.004651426358.06980528
Produced by FastQC (version 0.11.5)