FastQCFastQC Report
Fri 17 Jun 2016
SRR1526458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86862
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9211.0603025488706224No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC5170.5951969791162994No Hit
CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.36149294282885497No Hit
CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2900.3338629089820635No Hit
CTCCATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG2390.27514908705763164No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2160.24867030462112316No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1860.21413276231263384No Hit
CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1860.21413276231263384No Hit
CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1740.20031774538923813No Hit
CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1730.1991664939789551No Hit
CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1690.19456148833782322No Hit
CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.18880523128640833No Hit
CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT1530.17614146577329556No Hit
CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA1440.16578020308074878No Hit
CTCCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1410.16232644884989983No Hit
CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.16117519743961686No Hit
CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC1370.15772144320876794No Hit
CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1330.15311643756763602No Hit
CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1330.15311643756763602No Hit
CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1320.15196518615735305No Hit
CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1320.15196518615735305No Hit
CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1300.1496626833367871No Hit
CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1300.1496626833367871No Hit
CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1260.14505767769565517No Hit
CTCCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA1250.1439064262853722No Hit
CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1240.14275517487508924No Hit
CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG1220.14045267205452328No Hit
CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1220.14045267205452328No Hit
CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1210.13930142064424028No Hit
CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC1180.13584766641339135No Hit
CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAG1180.13584766641339135No Hit
CTCCATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1170.1346964150031084No Hit
CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG1160.1335451635928254No Hit
CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1140.13124266077225943No Hit
CTCCATGGGAACACCGGAAATGGAATCAAGTGACCTAAACACTTGACTCCACACTC1090.12548640372084455No Hit
CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1090.12548640372084455No Hit
CTCCATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT1060.12203264948999562No Hit
CTCCATGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG1020.11742764384886373No Hit
CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1020.11742764384886373No Hit
CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT970.11167138679744884No Hit
CTCCATGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAA920.10591512974603395No Hit
CTCCATGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG910.10476387833575096No Hit
CTCCATGGGTACTATTATTTGTTTTCAATGTATAAAGGCAGTATTTCCCTTTTCAG910.10476387833575096No Hit
CTCCATGGGCACGTTCATTCTCATCTCCGAAAACTATATCTCAGACCCAACTATCC870.10015887269461907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCAT150.0080117350.60902835
TTGCATC150.0080117350.60902836
GCAAACT251.9279707E-550.60902437
TATGCTT251.9279707E-550.60902435
CTATATC150.00803032650.5793834
TTTGGTG150.00803032650.5793834
ACTATAT150.008105027550.46113633
AACTATA150.00812378350.4316632
TGCGATC203.9892254E-450.43165632
AAACTAT150.00814257250.40221831
GTGCGAT204.0007493E-450.40221831
TGAGTTA150.00818024550.3434448
TGTCAAA150.0081991350.31410649
ACTATCC150.0081991350.31410650
CATTCTA150.0081991350.31410645
GGCCCTA150.0081991350.31410646
TGCCTTA150.0081991350.31410647
CCAACTA150.0081991350.31410647
GTGCCTT150.0081991350.31410646
TATTATA251.9958921E-550.31410249
Produced by FastQC (version 0.11.5)