##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526458_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86862 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.618037807096314 39.0 39.0 40.0 36.0 40.0 2 38.06643871888743 39.0 38.0 40.0 35.0 40.0 3 38.4259515092906 39.0 39.0 40.0 35.0 40.0 4 38.458002348552874 39.0 39.0 40.0 36.0 40.0 5 37.96651009647487 39.0 38.0 40.0 35.0 40.0 6 38.18175957265548 39.0 38.0 40.0 35.0 40.0 7 38.28175727015265 39.0 38.0 40.0 36.0 40.0 8 38.54236605189841 39.0 38.0 40.0 36.0 40.0 9 38.4869563215215 39.0 38.0 40.0 36.0 40.0 10 37.7441113490364 39.0 38.0 40.0 34.0 40.0 11 37.81143653150975 39.0 38.0 40.0 35.0 40.0 12 37.78901015403744 39.0 38.0 40.0 34.0 40.0 13 37.76981879302802 39.0 38.0 40.0 34.0 40.0 14 37.75260758444429 39.0 38.0 40.0 34.0 40.0 15 37.77962745504363 39.0 38.0 40.0 34.0 40.0 16 37.64306601275587 39.0 38.0 40.0 33.0 40.0 17 37.560129861159076 39.0 38.0 40.0 33.0 40.0 18 37.51239897768875 39.0 38.0 40.0 33.0 40.0 19 37.310722755635375 39.0 37.0 40.0 33.0 40.0 20 37.329637816306324 39.0 37.0 40.0 33.0 40.0 21 37.21270521056388 39.0 37.0 40.0 33.0 40.0 22 37.1004927356036 39.0 37.0 40.0 33.0 40.0 23 36.90654141051323 39.0 36.0 40.0 32.0 40.0 24 36.78127374456034 39.0 36.0 40.0 32.0 40.0 25 36.65571826575488 39.0 36.0 40.0 31.0 40.0 26 36.11244272524234 38.0 36.0 40.0 30.0 40.0 27 35.99726002164353 38.0 35.0 40.0 30.0 40.0 28 35.686583316064564 38.0 35.0 40.0 29.0 40.0 29 35.551633625751194 38.0 35.0 40.0 29.0 40.0 30 35.325527848771614 38.0 35.0 40.0 29.0 40.0 31 34.33995302894246 38.0 33.0 39.0 25.0 40.0 32 34.07288572678502 38.0 33.0 39.0 24.0 40.0 33 33.97379751790196 38.0 33.0 39.0 23.0 40.0 34 34.08345421473141 38.0 33.0 39.0 24.0 40.0 35 33.81597246206626 38.0 33.0 39.0 23.0 40.0 36 32.878819276553614 38.0 32.0 39.0 10.0 40.0 37 32.31475213557137 37.0 32.0 39.0 4.0 40.0 38 32.39833298795791 37.0 32.0 39.0 4.0 40.0 39 32.25479496212383 37.0 32.0 39.0 4.0 40.0 40 31.747611153323664 36.0 31.0 39.0 4.0 40.0 41 31.093320439317537 36.0 30.0 39.0 4.0 40.0 42 30.954859432202806 36.0 30.0 39.0 4.0 40.0 43 30.780064930579538 36.0 30.0 39.0 4.0 40.0 44 30.29934839170178 35.0 29.0 39.0 4.0 40.0 45 30.203287974027766 35.0 29.0 39.0 4.0 40.0 46 29.562225138725793 35.0 28.0 38.0 4.0 40.0 47 29.287237226865603 35.0 27.0 38.0 4.0 40.0 48 28.895224609150148 35.0 26.0 38.0 4.0 40.0 49 28.72118993345767 35.0 26.0 38.0 4.0 40.0 50 28.3319748566692 34.0 25.0 38.0 4.0 40.0 51 27.644861964955908 34.0 21.0 38.0 4.0 40.0 52 27.381075729317768 34.0 18.0 38.0 4.0 40.0 53 27.012099652322075 33.0 13.0 38.0 4.0 40.0 54 26.69175243489673 33.0 4.0 38.0 4.0 40.0 55 26.229202643273236 33.0 4.0 38.0 4.0 40.0 56 15.607296631438373 19.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.04841293018350967 1 2 -0.13468239019947958 1 3 -0.5900982085596169 1 4 -0.5095645897369323 1 5 -0.18111657081053778 1 6 -0.06260513657313282 1 7 0.19077822524246102 1 8 -0.42171001207240977 1 9 -0.5257203955642353 1 10 -0.1958612846167327 1 11 -0.07143155921269795 1 12 -0.3554087500791283 1 13 -0.26030447525695166 1 14 -0.02867549926889268 1 15 -0.33963785450800543 1 16 -0.7090190893962287 1 17 -0.9832175506262999 1 18 -0.6580621006513283 1 19 -0.5919582220745383 1 20 -0.5059352165761908 1 21 -0.511001444313564 1 22 -0.2749863511479944 1 23 0.19849386563134885 1 24 -0.4632948964874828 1 25 -0.05312301294706856 1 26 -0.11267251123851452 1 27 -0.6276834013120265 1 28 0.010548171983948862 1 29 -0.7998859656104571 1 30 0.38332225316990076 1 31 0.6343908408676526 1 32 0.8941775568687476 1 33 0.550535186583005 1 34 0.611546326011819 1 35 0.7571568129620942 1 36 0.7109642652906629 1 37 1.272663519379897 1 38 1.6846349159733123 1 39 0.9560584571590809 1 40 1.3209515319147584 1 41 1.330076100520337 1 42 1.662850032808663 1 43 1.8418314412744436 1 44 2.1087346821328232 1 45 2.656036214577405 1 46 2.5270136764793385 1 47 3.1356407187088635 1 48 3.309173151765574 1 49 2.8180559012034365 1 50 2.984387085634637 1 51 1.640725223276423 1 52 1.8265129718067712 1 53 2.2278349805491047 1 54 2.797701278735449 1 55 2.43234392141137 1 56 2.549942133627482 2 1 -0.013988725181960149 2 2 0.16896070952265774 2 3 0.31542818847650267 2 4 0.3190862284168219 2 5 0.29511065981619566 2 6 0.10195182545218273 2 7 0.449808790231657 2 8 0.3385555019751365 2 9 0.020590194123940364 2 10 0.12507110192232318 2 11 -0.30283013902128175 2 12 -0.24503517798897434 2 13 -0.16536776639619433 2 14 0.14282805120965492 2 15 -0.6818731123036201 2 16 -0.31553344567902997 2 17 -0.5035942100890978 2 18 -0.54043320284336 2 19 -0.6413561844085933 2 20 -0.21803771734803234 2 21 -0.9492539913959916 2 22 -0.7047486234542575 2 23 -1.0580668012411394 2 24 -0.3506057949129229 2 25 -0.4895231364419743 2 26 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0.4233494376468556 3 11 0.19975235739191533 3 12 0.0810767301889257 3 13 -0.04183835463148711 3 14 0.07869605694853021 3 15 0.3647552951569253 3 16 -0.20864678857716257 3 17 0.34217336523371245 3 18 0.0865395374722766 3 19 -0.016678996460242956 3 20 0.10836113487578558 3 21 0.3257818766097458 3 22 0.26053114699050184 3 23 0.2093650495479551 3 24 0.18942289949166735 3 25 -0.7186479571019433 3 26 -0.8720172617969695 3 27 -0.37496560533287493 3 28 -0.24231854432282773 3 29 0.04687501726370158 3 30 -0.5700656842835627 3 31 -0.3568228598024845 3 32 -0.9114814155809938 3 33 -0.6482734507959975 3 34 0.4617324764213464 3 35 -0.7031559197259227 3 36 0.4796909964894738 3 37 1.3092979199755774 3 38 0.16966842304702823 3 39 -0.25198324053265253 3 40 0.3289932296064926 3 41 -0.343937302309147 3 42 -0.04809561127175499 3 43 -0.2510948431633828 3 44 -0.49200991950529627 3 45 0.03488952805469481 3 46 -0.8965157352853659 3 47 -1.0143220512092341 3 48 -0.6229191788375559 3 49 -0.16437150013684843 3 50 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24 0.8020590650690096 120 25 0.5000884263403194 120 26 1.2225361151093495 120 27 0.2426088318494024 120 28 1.391232653934253 120 29 1.220585983131535 120 30 1.0498326454186895 120 31 1.0736782295221303 120 32 1.2337618669171917 120 33 1.184696774331094 120 34 0.4768377778011583 120 35 1.168158531980687 120 36 0.7617534511009509 120 37 0.5200459228804419 120 38 0.41396762420897915 120 39 0.9285541596125881 120 40 1.0521595331649536 120 41 1.349454128337186 120 42 1.3071694577187998 120 43 1.7229066092694438 120 44 1.5364577638643446 120 45 1.0848532172340697 120 46 0.4899766394423004 120 47 0.5235138238815438 120 48 1.1971243941471954 120 49 0.6225566044383157 120 50 1.4368015253908482 120 51 1.129551602398159 120 52 1.8572990321286937 120 53 1.8762175403240704 120 54 2.168540472359453 120 55 2.2831018523380777 120 56 1.8177964858700193 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 154.0 5 15.0 6 30.0 7 35.0 8 90.0 9 84.0 10 100.0 11 161.0 12 161.0 13 162.0 14 269.0 15 337.0 16 390.0 17 478.0 18 615.0 19 664.0 20 698.0 21 914.0 22 937.0 23 953.0 24 1424.0 25 1897.0 26 1669.0 27 1521.0 28 1818.0 29 1852.0 30 2114.0 31 2538.0 32 2860.0 33 3660.0 34 4704.0 35 6446.0 36 9360.0 37 13479.0 38 15352.0 39 8921.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.213133640553 0.4539170506912442 0.1947004608294931 0.1382488479262673 8 99.40250051806314 0.2981741152632912 0.18535147705555938 0.11397388961801479 9 97.98300752918422 1.2951578365683498 0.28551034975017847 0.4363242844972485 10 61.4975478344961 25.740830282517095 4.210126407404849 8.551495475581957 11 31.58343118970321 30.213442011466462 16.690842946282608 21.51228385254772 12 36.0813704496788 15.925260758444429 26.540950012663767 21.452418779213005 13 24.274135985816585 15.371508830098318 27.478068660634108 32.87628652345099 14 20.934355644585665 22.6025189380857 32.27072828164215 24.19239713568649 15 27.232852110243833 18.285326149524533 27.37790978793949 27.103911952292144 16 26.375169809583017 20.20906725610739 27.030231862034032 26.385531072275565 17 31.629481246114526 22.68771154244664 20.072068338283714 25.61073887315512 18 31.73539637586056 20.396721235983513 24.900416753010525 22.967465635145405 19 31.38311344431397 20.340309916879647 24.221178420943566 24.055398217862816 20 28.395616034629644 26.019433123805573 21.834634247426955 23.75031659413783 21 29.8392853031245 18.94844696184753 28.653496350533032 22.558771384494946 22 27.399783564734864 19.917800649305796 23.94718058529622 28.735235200663123 23 27.49220018189982 25.487848401469016 23.35110118465134 23.668850231979828 24 25.690470982374137 28.425875824593316 21.36056458019134 24.523088612841207 25 26.869694421727964 22.325758186572063 22.019373639410727 28.785173752289246 26 29.23309067453004 21.02607814227792 22.7734518982676 26.967379284924437 27 25.090749853071664 19.164064210562707 24.18843702824481 31.556748908120813 28 25.22789346916668 25.13224159588812 21.78903575997142 27.850829174973786 29 28.97048651141342 22.73922065944201 22.689647221581737 25.600645607562832 30 26.62444044487517 24.044718261110344 23.459781254326455 25.871060039688032 31 29.93735651411497 21.058824886653362 24.899343570101866 24.104475029129798 32 30.359389972177002 21.158175458040386 26.100509126173243 22.38192544360937 33 31.100821025646948 20.997933001535817 24.788969849536368 23.11227612328087 34 27.596840135818724 22.43434273439124 27.922527891345023 22.046289238445013 35 25.647482845458956 28.573573920476864 26.152299979206617 19.626643254857566 36 28.998520778440344 25.27850968427865 23.96454490824204 21.75842462903897 37 26.6211445018003 25.559608012211747 26.06066255724257 21.758584928745385 38 29.759550327885915 23.938563316100527 25.15295559950499 21.148930756508562 39 27.3428644134453 22.674485853748813 24.860619520207276 25.122030212598606 40 27.407630685045852 22.95349942613353 26.72362822727431 22.91524166154631 41 24.31393881873633 22.758365258857165 27.092906167895464 25.834789754511046 42 23.057951716551845 21.53650205523071 29.21438082556591 26.19116540265154 43 23.559809055939194 20.094313389257078 30.990174723084767 25.35570283171896 44 24.650866315118503 20.69305209480211 28.57391203569566 26.08216955438373 45 24.656073618209845 22.47372019980761 26.97055039810855 25.899655783874 46 24.506735294799565 22.25661357260439 28.188541884027728 25.048109248568316 47 26.282541669564413 22.394927788209657 28.256485151957715 23.066045390268215 48 24.96141303717114 24.22448908540194 27.305643560909377 23.50845431651754 49 26.323239354913564 23.69764473836872 26.516997331476972 23.462118575240748 50 22.83138401559454 24.772579597141 27.43316624895572 24.962870138308734 51 22.664686981637647 26.68282893897389 27.12129824032294 23.53118583906553 52 25.05541178791501 25.68786046673552 27.05951980318661 22.19720794216286 53 25.104748198098864 24.848245685302757 26.79000452651493 23.25700159008345 54 24.77973696124067 23.5539252672757 27.0340232393467 24.632314532136927 55 25.098671991084696 23.76021545319465 25.943768573551264 25.19734398216939 56 24.558266966959994 23.440293482551255 26.08256518609673 25.918874364392018 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 1.0 8 9.0 9 17.0 10 127.0 11 237.0 12 241.0 13 242.0 14 239.0 15 212.5 16 186.0 17 230.5 18 275.0 19 408.0 20 541.0 21 511.5 22 540.0 23 598.0 24 653.0 25 708.0 26 853.5 27 999.0 28 1197.5 29 1545.5 30 1695.0 31 1711.5 32 1728.0 33 1612.5 34 1497.0 35 1871.5 36 2246.0 37 1751.5 38 1206.5 39 1156.0 40 1245.0 41 1334.0 42 1415.5 43 1497.0 44 1592.5 45 1688.0 46 1903.0 47 2151.0 48 2184.0 49 2240.0 50 2296.0 51 2542.0 52 2788.0 53 3361.0 54 4319.0 55 4704.0 56 5020.5 57 5337.0 58 5761.5 59 6186.0 60 7203.5 61 8221.0 62 7794.5 63 6631.0 64 5894.0 65 5281.0 66 4668.0 67 4098.0 68 3528.0 69 3069.0 70 2610.0 71 2168.0 72 1533.5 73 1341.0 74 1102.5 75 864.0 76 691.0 77 518.0 78 379.5 79 207.5 80 174.0 81 120.5 82 67.0 83 52.0 84 37.0 85 27.0 86 17.0 87 13.5 88 8.5 89 7.0 90 4.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.07137758743754462 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0011512514102829776 24 0.0011512514102829776 25 0.049503810642168036 26 0.05295756487301698 27 0.09785636987405309 28 0.10246137551518501 29 0.14045267205452328 30 0.21413276231263384 31 0.20837650526121893 32 0.27975409269876356 33 0.3027791209044231 34 0.3177453892381018 35 0.34077041744376135 36 0.3799129653933826 37 1.2007552209251455 38 0.4593493127029081 39 0.4697105753954549 40 0.6965071032212014 41 0.5330294029610186 42 0.5721719509106399 43 0.6377932812967696 44 0.6642720637332781 45 0.6665745665538441 46 0.6907508461697865 47 0.6769358292463908 48 0.7978172273261034 49 0.773640947710161 50 0.7805484561718588 51 0.7517671709147844 52 0.7932122216849715 53 0.8093297414289333 54 0.823144758352329 55 0.8265985125831778 56 0.8335060210448757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 86862.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.170776634201374 #Duplication Level Percentage of deduplicated Percentage of total 1 71.37790341075761 30.81439524763418 2 11.586975652684071 10.004374755359075 3 5.440145070535214 7.045658630931823 4 3.152084055574815 5.443116667817918 5 1.9760526947385264 4.265386475098432 6 1.3680364809728258 3.543551840851005 7 1.0346942585135603 3.126798830328567 8 0.7360196271900584 2.5419631139048144 9 0.5093469159177578 1.9790011742764384 >10 2.570735219605856 19.129193433261957 >50 0.14400384010240275 4.157168842531831 >100 0.09866929784794261 6.293891460017038 >500 0.005333475559348249 1.6554995279869218 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTCCATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 921 1.0603025488706224 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 517 0.5951969791162994 No Hit CTCCATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 314 0.36149294282885497 No Hit CTCCATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 290 0.3338629089820635 No Hit CTCCATGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG 239 0.27514908705763164 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 216 0.24867030462112316 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 186 0.21413276231263384 No Hit CTCCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 186 0.21413276231263384 No Hit CTCCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 174 0.20031774538923813 No Hit CTCCATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 173 0.1991664939789551 No Hit CTCCATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 169 0.19456148833782322 No Hit CTCCATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 164 0.18880523128640833 No Hit CTCCATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 153 0.17614146577329556 No Hit CTCCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA 144 0.16578020308074878 No Hit CTCCATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 141 0.16232644884989983 No Hit CTCCATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.16117519743961686 No Hit CTCCATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC 137 0.15772144320876794 No Hit CTCCATGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 133 0.15311643756763602 No Hit CTCCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 133 0.15311643756763602 No Hit CTCCATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 132 0.15196518615735305 No Hit CTCCATGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 132 0.15196518615735305 No Hit CTCCATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 130 0.1496626833367871 No Hit CTCCATGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 130 0.1496626833367871 No Hit CTCCATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 126 0.14505767769565517 No Hit CTCCATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 125 0.1439064262853722 No Hit CTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 124 0.14275517487508924 No Hit CTCCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG 122 0.14045267205452328 No Hit CTCCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 122 0.14045267205452328 No Hit CTCCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 121 0.13930142064424028 No Hit CTCCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 118 0.13584766641339135 No Hit CTCCATGGGGCTCTCCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAG 118 0.13584766641339135 No Hit CTCCATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 117 0.1346964150031084 No Hit CTCCATGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG 116 0.1335451635928254 No Hit CTCCATGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 114 0.13124266077225943 No Hit CTCCATGGGAACACCGGAAATGGAATCAAGTGACCTAAACACTTGACTCCACACTC 109 0.12548640372084455 No Hit CTCCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 109 0.12548640372084455 No Hit CTCCATGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 106 0.12203264948999562 No Hit CTCCATGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG 102 0.11742764384886373 No Hit CTCCATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 102 0.11742764384886373 No Hit CTCCATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT 97 0.11167138679744884 No Hit CTCCATGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAA 92 0.10591512974603395 No Hit CTCCATGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG 91 0.10476387833575096 No Hit CTCCATGGGTACTATTATTTGTTTTCAATGTATAAAGGCAGTATTTCCCTTTTCAG 91 0.10476387833575096 No Hit CTCCATGGGCACGTTCATTCTCATCTCCGAAAACTATATCTCAGACCCAACTATCC 87 0.10015887269461907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.002302502820565955 0.0 0.0 0.0 0.0 8 0.005756257051414888 0.0 0.0 0.0 0.0 9 0.008058759871980844 0.0 0.0 0.0 0.0 10 0.017268771154244664 0.0 0.0 0.0 0.0 11 0.05410881628329995 0.0 0.0 0.0 0.0 12 0.05410881628329995 0.0 0.0 0.0 0.0 13 0.0564113191038659 0.0 0.0 0.0 0.0 14 0.07368009025811056 0.0 0.0 0.0 0.0 15 0.0771338444889595 0.0 0.0 0.0 0.0 16 0.08173885013009141 0.0 0.0 0.0 0.0 17 0.08404135295065736 0.0 0.0 0.0 0.0 18 0.0921001128226382 0.0 0.0 0.0 0.0 19 0.10476387833575096 0.0 0.0 0.0 0.0 20 0.12433515231056158 0.0 0.0 0.0 0.0 21 0.12663765513112754 0.0 0.0 0.0 0.0 22 0.13239391218254243 0.0 0.0 0.0 0.0 23 0.14851143192650412 0.0 0.0 0.0 0.0 24 0.15311643756763602 0.0 0.0 0.0 0.0 25 0.16117519743961686 0.0 0.0 0.0 0.0 26 0.1749902143630126 0.0 0.0 0.0 0.0 27 0.17844396859386152 0.0 0.0 0.0 0.0 28 0.2106790080817849 0.0 0.0 0.0 0.0 29 0.2164352651331998 0.0 0.0 0.0 0.0 30 0.23946029333885935 0.0 0.0 0.0 0.0 31 0.24982155603140613 0.0 0.0 0.0 0.0 32 0.256729064493104 0.0 0.0 0.0 0.0 33 0.26248532154451887 0.0 0.0 0.0 0.0 34 0.2693928300062168 0.0 0.0 0.0 0.0 35 0.3223503948792337 0.0 0.0 0.0 0.0 36 0.32695540052036565 0.0 0.0 0.0 0.0 37 0.3304091547512146 0.0 0.0 0.0 0.0 38 0.3373166632129124 0.0 0.0 0.0 0.0 39 0.34652667449517627 0.0 0.0 0.0 0.0 40 0.3545854343671571 0.0 0.0 0.0 0.0 41 0.360341691418572 0.0 0.0 0.0 0.0 42 0.36264419423913796 0.0 0.0 0.0 0.0 43 0.3695517027008358 0.0 0.0 0.0 0.0 44 0.37761046257281666 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGCAT 15 0.00801173 50.609028 35 TTGCATC 15 0.00801173 50.609028 36 GCAAACT 25 1.9279707E-5 50.609024 37 TATGCTT 25 1.9279707E-5 50.609024 35 CTATATC 15 0.008030326 50.57938 34 TTTGGTG 15 0.008030326 50.57938 34 ACTATAT 15 0.0081050275 50.461136 33 AACTATA 15 0.008123783 50.43166 32 TGCGATC 20 3.9892254E-4 50.431656 32 AAACTAT 15 0.008142572 50.402218 31 GTGCGAT 20 4.0007493E-4 50.402218 31 TGAGTTA 15 0.008180245 50.34344 48 TGTCAAA 15 0.00819913 50.314106 49 ACTATCC 15 0.00819913 50.314106 50 CATTCTA 15 0.00819913 50.314106 45 GGCCCTA 15 0.00819913 50.314106 46 TGCCTTA 15 0.00819913 50.314106 47 CCAACTA 15 0.00819913 50.314106 47 GTGCCTT 15 0.00819913 50.314106 46 TATTATA 25 1.9958921E-5 50.314102 49 >>END_MODULE