FastQCFastQC Report
Fri 17 Jun 2016
SRR1526457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405864
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.3518419963337473No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC12730.3136518636784736No Hit
TTAACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA12430.3062602251000335No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT12410.30576744919480414No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9520.2345613308891648No Hit
TTAACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA6890.1697612993515069No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5530.13625253779591193No Hit
TTAACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5180.1276289594543985No Hit
TTAACTGGGGTGCTTTCATACAGTTACTGACTTATGAATGATTGATTTGACAGAGACCCAAGCT4980.12270120040210515No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4920.12122287268641711No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4780.11777344134981177No Hit
TTAACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4670.1150631738710504No Hit
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4420.10890347505568368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT150.00461160358.23188856
CGCCTAA150.004620671358.20300349
CGCTAGT258.51945E-658.1597344
ACGATAC150.00468911157.98727830
CGCGAGT150.004735154657.84434519
ACTGGGG175200.055.2651564
TAACTGG419450.055.224412
AACTGGG408700.055.170213
TTAACTG423550.055.1540031
ACTGGGT33550.054.979214
ACTGGGA143450.054.920734
CTGGGGG51850.054.588895
CTGGGGT48850.053.6208885
CTGGGAC28900.053.521265
TGGGATC9400.053.5176
CTGGGAT40450.053.3915375
ACTGGGC58950.053.3109054
CTGGGGA48850.052.5555735
CTGGGAG39200.052.4389425
CTGGGTA10800.051.9335865
Produced by FastQC (version 0.11.5)