FastQCFastQC Report
Fri 17 Jun 2016
SRR1526456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392899
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.3000771190560424No Hit
TTAACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA10640.2708075103270815No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT10480.26673521693870433No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT10220.2601177401825915No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC9830.2501915250484221No Hit
TTAACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA5540.14100315857255935No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4730.12038717329389996No Hit
TTAACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4300.10944288481263634No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4070.10358896306684415No Hit
TTAACTGGGGTGCTTTCATACAGTTACTGACTTATGAATGATTGATTTGACAGAGACCCAAGCT3970.10104377969910841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG201.9632735E-458.2691136
TACACCG150.004611543458.2317138
CACGTAA201.9858193E-458.13469355
TGACGCG150.004651520458.10490847
TAATACG150.004665691458.06028425
TTGTCGC150.004665691458.06028442
CGTAATC150.004665691458.06028441
TCGGTAT202.0225754E-457.91943714
AAATCGC150.00473702957.838217
CGTGACG150.004756188557.77926619
AGGCACG202.0469491E-457.77926620
ATCGCGG150.004756188557.77926619
AATCGCG150.004763388557.75719518
TAACTGG401750.055.5141142
ACTGGGG161550.055.4545024
AACTGGG390450.055.4337583
TTAACTG407450.055.40421
ACTGGGA145500.054.840664
ACTGGGT32450.054.7523044
ACTAGGG950.054.738254
Produced by FastQC (version 0.11.5)