##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526456_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392899 Sequences flagged as poor quality 0 Sequence length 64 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.483442309601195 39.0 38.0 40.0 35.0 40.0 2 38.4482195169751 39.0 38.0 40.0 36.0 40.0 3 38.06607296022642 39.0 38.0 40.0 35.0 40.0 4 38.29157111624107 39.0 38.0 40.0 35.0 40.0 5 38.28178234100876 39.0 38.0 40.0 35.0 40.0 6 38.26782710060346 39.0 38.0 40.0 35.0 40.0 7 38.48661615326076 39.0 38.0 40.0 36.0 40.0 8 38.375238929088646 39.0 38.0 40.0 36.0 40.0 9 38.53272214996729 39.0 38.0 40.0 36.0 40.0 10 37.75060511734568 39.0 38.0 40.0 33.0 40.0 11 37.8511118633542 39.0 38.0 40.0 35.0 40.0 12 37.76013682905785 39.0 38.0 40.0 33.0 40.0 13 37.68683554806706 39.0 38.0 40.0 33.0 40.0 14 37.64766008567087 39.0 38.0 40.0 33.0 40.0 15 37.42358977752552 39.0 38.0 40.0 33.0 40.0 16 37.26521065210143 39.0 38.0 40.0 33.0 40.0 17 36.948851995041984 39.0 37.0 40.0 32.0 40.0 18 37.26836158911069 39.0 37.0 40.0 33.0 40.0 19 37.194716708365256 39.0 37.0 40.0 33.0 40.0 20 37.11736095026966 39.0 37.0 40.0 32.0 40.0 21 37.169272510238 39.0 37.0 40.0 33.0 40.0 22 37.09227816818063 39.0 37.0 40.0 33.0 40.0 23 36.978775715896454 39.0 36.0 40.0 32.0 40.0 24 36.86154711516191 39.0 36.0 40.0 32.0 40.0 25 36.78598571134057 39.0 36.0 40.0 31.0 40.0 26 36.88966884619202 39.0 37.0 40.0 32.0 40.0 27 36.37370672870127 39.0 36.0 40.0 30.0 40.0 28 36.332528206994674 39.0 36.0 40.0 30.0 40.0 29 36.2626731042838 39.0 36.0 40.0 30.0 40.0 30 36.24336279807279 39.0 36.0 40.0 30.0 40.0 31 35.934156106276674 39.0 36.0 40.0 29.0 40.0 32 36.07221703287613 39.0 36.0 40.0 29.0 40.0 33 36.04066693984968 39.0 35.0 40.0 29.0 40.0 34 35.53289267725293 39.0 35.0 40.0 27.0 40.0 35 35.63068880297481 39.0 35.0 40.0 28.0 40.0 36 35.23897235676344 39.0 35.0 40.0 27.0 40.0 37 35.08343874634448 38.0 35.0 40.0 27.0 40.0 38 35.11326065986424 38.0 35.0 40.0 27.0 40.0 39 34.61947727023993 38.0 34.0 40.0 24.0 40.0 40 34.82488374874968 38.0 34.0 40.0 26.0 40.0 41 34.53648902135154 38.0 34.0 40.0 25.0 40.0 42 34.256628293785425 38.0 34.0 40.0 23.0 40.0 43 34.27015594338494 38.0 34.0 40.0 24.0 40.0 44 34.131873585832494 38.0 33.0 40.0 23.0 40.0 45 33.97828449550648 38.0 33.0 40.0 23.0 40.0 46 33.79608754412712 38.0 33.0 39.0 21.0 40.0 47 33.58896306684415 38.0 33.0 39.0 20.0 40.0 48 33.47754766492152 38.0 33.0 39.0 19.0 40.0 49 33.292825891641364 38.0 33.0 39.0 18.0 40.0 50 32.939282105579295 37.0 33.0 39.0 13.0 40.0 51 32.65776191845742 37.0 32.0 39.0 6.0 40.0 52 32.552164805713424 37.0 32.0 39.0 4.0 40.0 53 32.33543226121726 36.0 32.0 39.0 4.0 40.0 54 32.09347440436346 36.0 31.0 39.0 4.0 40.0 55 31.851114408537562 36.0 31.0 39.0 4.0 40.0 56 31.40986360362332 36.0 31.0 39.0 4.0 40.0 57 31.076027172377636 36.0 31.0 39.0 4.0 40.0 58 30.88879584829689 36.0 30.0 39.0 4.0 40.0 59 30.6332059893255 36.0 30.0 39.0 4.0 40.0 60 30.38604832285142 35.0 30.0 39.0 4.0 40.0 61 30.115174637756777 35.0 30.0 39.0 4.0 40.0 62 29.871058465407142 35.0 29.0 39.0 4.0 40.0 63 29.64167126920659 35.0 29.0 39.0 4.0 40.0 64 29.51449354668757 35.0 28.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.13113876152930004 2 2 0.05364412130208507 2 3 -0.387057883024319 2 4 0.13328982909860088 2 5 -0.19486222731660519 2 6 -0.10952420071593139 2 7 -0.019884405104626524 2 8 0.24994673209034346 2 9 -0.2085694873297257 2 10 -0.14102121132432188 2 11 -0.597948555188097 2 12 -0.22071686217815056 2 13 -0.31712660922418223 2 14 -0.5878280788561128 2 15 -0.2932913176023533 2 16 -0.43556229193450946 2 17 -0.13196984625501074 2 18 -0.5119739608254861 2 19 -0.4512521528210911 2 20 -0.36915208175119574 2 21 -0.5705172140012778 2 22 -1.194697756931184 2 23 -0.578969136232395 2 24 -0.835482845020664 2 25 -0.4377354208692381 2 26 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0.3598427109939557 119 62 0.4413440968218296 119 63 0.6352430232764803 119 64 0.6781706824812055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 308.0 5 32.0 6 84.0 7 146.0 8 398.0 9 286.0 10 438.0 11 544.0 12 634.0 13 899.0 14 1063.0 15 1137.0 16 1493.0 17 1666.0 18 1736.0 19 1983.0 20 2290.0 21 2676.0 22 3270.0 23 3386.0 24 3631.0 25 4303.0 26 4035.0 27 4855.0 28 5765.0 29 6649.0 30 8291.0 31 9983.0 32 12023.0 33 15598.0 34 20226.0 35 26625.0 36 37662.0 37 53915.0 38 74314.0 39 80285.0 40 270.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.48128898922619 0.3685444276744287 0.07966464493238685 0.07050193816700051 8 98.98137961944342 0.6997922014423664 0.205761316872428 0.11306686224177973 9 97.02900745484209 1.568341991198756 0.4894387616155806 0.9132117923435793 10 41.940040570222884 35.39331991173304 8.348455964509963 14.318183553534114 11 27.04384587387598 28.51165312204918 25.613452821208504 18.831048182866333 12 34.25867716639645 20.13952695221927 24.06445422360454 21.537341657779734 13 26.24033148468181 19.993433426911242 28.52794229560269 25.238292792804256 14 23.132917111013263 24.937452118737895 29.380833242130926 22.548797528117916 15 25.50853264926086 26.96388494743358 27.28155067749159 20.24603172581397 16 27.42467253077383 26.909123311735428 24.916684700281326 20.749519457209413 17 26.804630866324775 25.51462566654092 23.677113814092436 24.003629653041873 18 25.7300222194508 25.965960717639902 27.198338504297542 21.105678558611753 19 25.260944924777107 26.97665303296776 24.9364340453908 22.825967996864335 20 24.665297051253656 28.96482023161266 24.123051543872243 22.24683117326144 21 24.007951152840807 24.877385791259332 27.863140399950115 23.251522655949746 22 22.82418636850692 23.912761294887492 28.891139962178574 24.371912374427016 23 22.49662126907933 27.20953731111558 28.048429749121272 22.245411670683815 24 22.12578363423021 29.715104573649526 26.673216571939086 21.485895220181174 25 24.16418792274468 28.89154306902453 25.018081248472008 21.92618775975878 26 22.540101982887446 27.70950915325691 26.177983814098894 23.572405049756753 27 21.16107543445328 25.02078540787856 28.337014348307328 25.48112480936083 28 20.32774848821676 27.607507418700223 28.47939589855501 23.58534819452801 29 22.914160771441967 26.830492752441337 28.133982997288538 22.121363478828158 30 22.19688971177044 25.99472607735426 30.816743939324386 20.99164027155091 31 22.13682822372264 26.267539709059555 31.721588923550975 19.874043143666828 32 22.915870029622152 25.367982583602277 32.23002289216211 19.486124494613456 33 22.668479356002152 25.94764456665077 31.96573915128389 19.41813692606319 34 20.32997212762533 26.7518592227925 34.22553886165237 18.692629787929803 35 18.86322642862063 29.21225913273754 33.74399506083389 18.18051937780794 36 19.793637540502655 28.700263761965676 32.224664935489386 19.28143376204228 37 20.456867312328882 28.066640104613622 32.564310816885296 18.912181766172203 38 20.397677067425317 28.17398499389643 32.976908579221295 18.45142935945696 39 19.969611015878627 27.342142551496 32.59615704904003 20.09208938358534 40 20.78919606525887 26.539834017476956 33.46573449437657 19.205235422887608 41 18.002714092580714 28.34897812625386 33.53948851366375 20.10881926750168 42 18.121581449132986 27.240957629156625 33.92353702651951 20.713923895190874 43 18.929537978277665 27.343490205507837 34.3157668557864 19.4112049604281 44 19.637647624331713 26.902081506273156 33.38745266688996 20.07281820250517 45 19.3169988054769 27.982279109146667 32.07997933244831 20.620742752928116 46 18.674222557660574 28.170084300205957 32.36360380182416 20.792089340309314 47 18.201425044452687 29.406572601793457 33.829583104140816 18.562419249613036 48 19.62101407384291 29.111777315072978 32.72488220638567 18.542326404698443 49 20.58311096556647 28.21661477374773 32.63318400794128 18.567090252744524 50 18.743980656134095 28.81370258806172 32.85562795844347 19.58668879736071 51 17.47673454344421 30.023188672870788 33.41446809381943 19.085608689865577 52 18.43193872744773 29.391933402961236 32.96241117323012 19.21371669636092 53 19.894613673082194 28.845026282571567 32.74375708912519 18.516602955221053 54 18.710174572656683 29.345307735829252 31.80657994782533 20.137937743688735 55 20.138287792261007 29.322133729416887 31.611821045353274 18.927757432968832 56 18.971756997999655 29.715725873581384 30.831154128877426 20.481362999541535 57 18.752705246577623 31.29173122975067 30.70061084147575 19.254952682195956 58 19.7866937271453 32.14661859331637 29.48336523702263 18.5833224425157 59 20.236231167240287 31.926573059191053 29.661132054196322 18.17606371937234 60 21.043806867271883 31.126795589317346 29.37318774628063 18.456209797130132 61 20.28769460111574 30.72558873401056 29.10346452739912 19.883252137474578 62 18.491343311113045 31.245292493095654 29.76707127741883 20.496292918372472 63 18.704798210758504 31.523084135853235 29.246054010293776 20.526063643094485 64 19.3335213868089 31.294491742742846 29.46815057954662 19.90383629090163 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 1.5 2 10.5 3 18.0 4 48.0 5 308.5 6 539.0 7 1105.5 8 1672.0 9 2015.0 10 2487.5 11 2617.0 12 2886.5 13 3452.5 14 3749.0 15 4003.0 16 4715.0 17 5173.0 18 5856.5 19 6540.0 20 7082.0 21 8311.0 22 8998.0 23 9229.5 24 9408.0 25 9355.0 26 8659.5 27 8451.5 28 8939.0 29 9728.5 30 9906.0 31 9294.0 32 9270.0 33 9246.0 34 9447.5 35 9874.5 36 10100.0 37 10771.5 38 11787.5 39 12132.0 40 11987.0 41 12416.0 42 12990.0 43 13639.0 44 14288.0 45 14554.5 46 15570.0 47 16319.0 48 16642.0 49 17120.0 50 17275.0 51 17424.5 52 17632.5 53 17691.0 54 17168.0 55 17026.0 56 17407.0 57 17247.5 58 17088.0 59 16041.5 60 12731.5 61 10468.0 62 8761.5 63 5902.0 64 4749.0 65 4016.5 66 2782.0 67 2280.0 68 1879.5 69 1479.0 70 1342.5 71 957.0 72 708.0 73 531.5 74 291.5 75 228.0 76 184.0 77 108.0 78 76.0 79 59.5 80 30.0 81 17.0 82 16.0 83 15.0 84 10.5 85 4.5 86 3.0 87 3.0 88 1.5 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 5.090366735471457E-4 8 0.05370336905922387 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08857238119720334 16 0.029015090392187304 17 0.1463480436448044 18 0.0 19 0.0 20 0.022397613636074412 21 0.0 22 0.0 23 0.0 24 0.004581330061924311 25 0.057775662447601045 26 0.022397613636074412 27 0.2028511144085376 28 0.16467336389250165 29 0.12496850335582427 30 0.1987788210201604 31 0.21888576962527267 32 0.15907396048348302 33 0.21583154958398978 34 0.3746509917306992 35 0.23670205319942272 36 0.32018406766115465 37 0.3469084930223798 38 0.33723679622498404 39 0.6683651523674023 40 0.35937989152428484 41 0.4092654855319051 42 0.5133634852722964 43 0.5001285317600707 44 0.4550787861511483 45 0.49580172003491996 46 0.5176902969974472 47 0.5176902969974472 48 0.49911045841297635 49 0.5166722236503529 50 0.63400517690297 51 0.5576496758708981 52 0.5729207760773125 53 0.5945548347030662 54 0.5823379545379347 55 0.613134673287537 56 0.6276422184836307 57 0.6245879984423478 58 0.6775278124912509 59 0.6841452892473638 60 0.7243591864575883 61 0.6324780668823285 62 0.6528395338242143 63 0.6530940521609879 64 0.7480293917775306 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 392899.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.27503108405357 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4930563675018 50.67603573654414 2 4.940815752102674 5.857340145729351 3 2.5254203261892245 4.490831050055011 4 1.6854755548271279 3.9962646361516176 5 1.2000985217047218 3.5567938588987063 6 0.9187937324883917 3.267691623184982 7 0.6979171704930209 2.8958343382547977 8 0.49089960884780226 2.3278471658882576 9 0.39401464704099637 2.1019707405834716 >10 1.5780798115716728 14.8967689452588 >50 0.04862649959255737 1.9125556073601107 >100 0.024504692699421425 2.692375360596898 >500 7.657716468569195E-4 0.34883089684206625 >1k 0.001531543293713839 0.9788598946518099 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.3000771190560424 No Hit TTAACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA 1064 0.2708075103270815 No Hit TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 1048 0.26673521693870433 No Hit TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 1022 0.2601177401825915 No Hit TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 983 0.2501915250484221 No Hit TTAACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA 554 0.14100315857255935 No Hit TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 473 0.12038717329389996 No Hit TTAACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 430 0.10944288481263634 No Hit TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 407 0.10358896306684415 No Hit TTAACTGGGGTGCTTTCATACAGTTACTGACTTATGAATGATTGATTTGACAGAGACCCAAGCT 397 0.10104377969910841 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.635550103207185E-4 0.0 0.0 0.0 0.0 8 7.635550103207185E-4 0.0 0.0 0.0 0.0 9 7.635550103207185E-4 0.0 0.0 0.0 0.0 10 0.004835848398697884 0.0 0.0 0.0 0.0 11 0.00890814178707505 0.0 0.0 0.0 0.0 12 0.010180733470942914 0.0 0.0 0.0 0.0 13 0.011453325154810779 0.0 0.0 0.0 0.0 14 0.01679821022705581 0.0 0.0 0.0 0.0 15 0.017052728563829382 0.0 0.0 0.0 0.0 16 0.018579838584470817 0.0 0.0 0.0 0.0 17 0.02137954028898012 0.0 0.0 0.0 0.0 18 0.025706352014130858 0.0 0.0 0.0 0.0 19 0.030796718749602315 0.0 0.0 0.0 0.0 20 0.034359975464432334 0.0 0.0 0.0 0.0 21 0.037159677168941634 0.0 0.0 0.0 0.0 22 0.04021389721022451 0.0 0.0 0.0 0.0 23 0.046067818956016686 0.0 0.0 0.0 0.0 24 0.05090366735471457 0.0 0.0 0.0 0.0 25 0.055484997416638884 0.0 0.0 0.0 0.0 26 0.05803018078437461 0.0 0.0 0.0 0.0 27 0.062102474172751776 0.0 0.0 0.0 0.0 28 0.0722832076436947 0.0 0.0 0.0 0.0 29 0.0778826110527133 0.0 0.0 0.0 0.0 30 0.08373653279850547 0.0 0.0 0.0 0.0 31 0.08704527117656191 0.0 0.0 0.0 0.0 32 0.08959045454429765 0.0 0.0 0.0 0.0 33 0.09391726626944838 0.0 0.0 0.0 0.0 34 0.09875311466814626 0.0 0.0 0.0 0.0 35 0.11453325154810778 0.0 0.0 0.0 0.0 36 0.12140524664099425 0.0 0.0 0.0 0.0 37 0.12725916838678641 0.0 0.0 0.0 0.0 38 0.13260405345903145 0.0 0.0 0.0 0.0 39 0.13820345686805005 0.0 0.0 0.0 0.0 40 0.14405737861384224 0.0 0.0 0.0 0.0 41 0.1473661169918987 0.0 0.0 0.0 0.0 42 0.15398359374801157 0.0 0.0 0.0 0.0 43 0.1583104054731623 0.0 0.0 0.0 0.0 44 0.16263721719831306 0.0 0.0 0.0 0.0 45 0.16747306559701094 0.0 0.0 0.0 0.0 46 0.1735815056795767 0.0 0.0 0.0 0.0 47 0.17943542742536886 0.0 0.0 0.0 0.0 48 0.1845257941608403 0.0 0.0 0.0 0.0 49 0.19012519756985893 0.0 0.0 0.0 0.0 50 0.1967426743259718 0.0 0.0 0.0 0.0 51 0.1997968943672547 0.0 0.0 0.0 0.0 52 0.20666888946014117 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACG 20 1.9632735E-4 58.26911 36 TACACCG 15 0.0046115434 58.23171 38 CACGTAA 20 1.9858193E-4 58.134693 55 TGACGCG 15 0.0046515204 58.104908 47 TAATACG 15 0.0046656914 58.060284 25 TTGTCGC 15 0.0046656914 58.060284 42 CGTAATC 15 0.0046656914 58.060284 41 TCGGTAT 20 2.0225754E-4 57.919437 14 AAATCGC 15 0.004737029 57.8382 17 CGTGACG 15 0.0047561885 57.779266 19 AGGCACG 20 2.0469491E-4 57.779266 20 ATCGCGG 15 0.0047561885 57.779266 19 AATCGCG 15 0.0047633885 57.757195 18 TAACTGG 40175 0.0 55.514114 2 ACTGGGG 16155 0.0 55.454502 4 AACTGGG 39045 0.0 55.433758 3 TTAACTG 40745 0.0 55.4042 1 ACTGGGA 14550 0.0 54.84066 4 ACTGGGT 3245 0.0 54.752304 4 ACTAGGG 95 0.0 54.73825 4 >>END_MODULE