FastQCFastQC Report
Fri 17 Jun 2016
SRR1526455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347606
Sequences flagged as poor quality0
Sequence length56
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAACTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14960.43037231808426785No Hit
TTAACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12360.3555749900749699No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC12280.35327353382853No Hit
TTAACTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT10640.30609368077651133No Hit
TTAACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7830.22525503012030862No Hit
TTAACTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC5910.17002008020575018No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC5740.1651294856820653No Hit
TTAACTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5570.16023889115838047No Hit
TTAACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5210.14988233804940077No Hit
TTAACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.1377996927555911No Hit
TTAACTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG4340.12485400136936647No Hit
TTAACTGGGGTGCTTTCATACAGTTACTGACTTATGAATGATTGATTTGACAGAGA4170.11996340684568162No Hit
TTAACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC3940.1133467201371668No Hit
TTAACTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3560.10241480296657711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAAC150.00801654350.63408734
AGCGTTG150.00812389850.46431
AGGCTCG150.00825602850.25847644
CCGTCAT204.0864974E-450.22924439
ACGGTTT150.00846204849.94608328
CCCCACG150.008495945549.895627
TACGCTG150.00853480849.8380224
CCGAATT150.00862763649.70181311
GGCGACT150.00862763649.7018138
TGGGCGA1350.047.8816646
TAACTGG355650.047.8638042
AACTGGG345600.047.8456953
TTAACTG358800.047.8316271
ACTGGGG149200.047.7403374
ACTGGGA123700.047.6933364
CTGGGAT34350.046.900555
CTGGGGT41750.046.8053785
AGACACG1450.046.76516339
ACTGGGT28100.046.6266254
ACTGGGC47250.046.6188554
Produced by FastQC (version 0.11.5)