FastQCFastQC Report
Fri 17 Jun 2016
SRR1526454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463052
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC23470.5068545217383793No Hit
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.49908001693114384No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT16220.35028463325933157No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16090.3474771731900521No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT14740.3183227801629191No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC14430.3116280676900218No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.294783307274345No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10310.22265317934054923No Hit
TAGAGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG10060.2172542176688579No Hit
TAGAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC8840.19090728471100438No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG8570.18507640610557777No Hit
TAGAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA8390.18118915370196004No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.17449444122906282No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT7840.16931143802423917No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA6490.14015704499710616No Hit
TAGAGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC6360.13734958492782667No Hit
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC6300.13605383412662075No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC5800.12525591078323817No Hit
TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG5680.12266440918082636No Hit
TAGAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC5400.11661757210853207No Hit
TAGAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC5340.11532182130732618No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC5130.11078669350310548No Hit
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.10733135803322304No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4960.10711539956635541No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.10603560723201715No Hit
TAGAGAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG4910.10603560723201715No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.10538773183141419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA258.519246E-658.16216350
CGATTGT303.6987876E-758.13061544
TAATAAC150.004647903658.11800439
CTATCTA150.004673905758.03617535
CTCGTCA150.00468393558.00476533
TCGAAAC150.004700015857.95457527
AGCGATC150.004706056357.93577626
TAATCGA150.00471008757.92325624
GTAATCG150.00471210357.91699223
ATGATAC150.004730277757.86070610
GGTCTAT150.004730277757.8607069
CTATAAC258.785757E-657.86070313
AGAGGGG256500.056.067364
GAGAGGG467500.055.8928223
TAGAGAG477200.055.87221
CCGTTAA1200.055.5698530
AGAGAGG477000.055.5681152
GAGGGAT26600.055.141695
GAGGGGT39050.055.119555
AGAGGGT29450.055.1101154
Produced by FastQC (version 0.11.5)