FastQCFastQC Report
Fri 17 Jun 2016
SRR1526453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446504
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19720.4416533782452117No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC19060.42687187572787705No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT16500.3695375629333668No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12370.2770411911203483No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC11470.2568845967785283No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT11300.2530772400695179No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.23471234300252627No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC8490.19014387329116872No Hit
TAGAGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG8230.18432085714797627No Hit
TAGAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6890.15430992779459982No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6600.14781502517334671No Hit
TAGAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA6570.14714313869528603No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6250.13997634959597224No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.13661691720566893No Hit
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC5370.12026767957285936No Hit
TAGAGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC5260.11780409581997026No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA4950.11086126888001005No Hit
TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG4780.10705391217099959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATAGT351.6088961E-858.1326146
CGAAGTA150.0046535458.09985444
AACCGAC150.00466601258.06059330
GCAATTC150.0046743458.03444727
GTCATTC150.00475401357.78724310
AGAGGGG248100.055.7287644
GAGAGGG449450.055.7120743
TAGAGAG461100.055.569331
AGAGAGG459600.055.2480772
GAGGGAT25000.055.201295
GAGGGGG102600.054.984285
AGAGGGA130150.054.880354
AGAGGGT27500.054.8023454
AGGGTAC3850.054.742356
AGGGGGG53700.054.1397826
AGGGAGT13000.053.96656
AGGGGCT27350.053.9417346
GAGGGGA60400.053.8701635
AGGGATC6650.053.8345876
GAGGGGT39100.053.828485
Produced by FastQC (version 0.11.5)