##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526453_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 446504 Sequences flagged as poor quality 0 Sequence length 64 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.02380941716088 39.0 38.0 40.0 35.0 40.0 2 37.56734094207443 39.0 38.0 40.0 33.0 40.0 3 37.78438491032555 39.0 38.0 40.0 34.0 40.0 4 36.57392990880261 39.0 36.0 40.0 31.0 40.0 5 37.5585168329959 39.0 38.0 40.0 33.0 40.0 6 36.991249798434055 39.0 36.0 40.0 33.0 40.0 7 38.067694802286205 39.0 38.0 40.0 35.0 40.0 8 37.94062091269059 39.0 38.0 40.0 35.0 40.0 9 38.11874697651085 39.0 38.0 40.0 35.0 40.0 10 36.77237381971942 39.0 35.0 40.0 31.0 40.0 11 37.014756866679804 39.0 36.0 40.0 32.0 40.0 12 37.08874724526544 39.0 36.0 40.0 32.0 40.0 13 37.11145700822389 39.0 36.0 40.0 32.0 40.0 14 37.027247236306955 39.0 36.0 40.0 32.0 40.0 15 36.896731048322074 39.0 36.0 40.0 32.0 40.0 16 36.68179680361206 39.0 36.0 40.0 31.0 40.0 17 36.31169037679393 38.0 36.0 40.0 30.0 40.0 18 36.58528926952502 38.0 36.0 40.0 31.0 40.0 19 36.49146032286385 38.0 36.0 40.0 31.0 40.0 20 36.394280902298746 38.0 35.0 40.0 31.0 40.0 21 36.405250568863885 38.0 36.0 40.0 31.0 40.0 22 36.29597047282891 38.0 35.0 40.0 30.0 40.0 23 36.11879624818591 38.0 35.0 40.0 30.0 40.0 24 35.98384113020264 38.0 35.0 40.0 30.0 40.0 25 35.90477576908606 38.0 35.0 40.0 29.0 40.0 26 36.077569293892104 39.0 35.0 40.0 30.0 40.0 27 35.529166592012615 38.0 35.0 40.0 28.0 40.0 28 35.43743393116299 38.0 35.0 40.0 28.0 40.0 29 35.33978419006325 38.0 35.0 40.0 28.0 40.0 30 35.23852641857632 38.0 35.0 40.0 27.0 40.0 31 34.866652930320896 38.0 35.0 40.0 26.0 40.0 32 35.04662892157741 38.0 35.0 40.0 27.0 40.0 33 34.937570548080195 38.0 34.0 39.0 27.0 40.0 34 34.24533710784226 38.0 33.0 39.0 23.0 40.0 35 34.32135658359164 38.0 33.0 39.0 24.0 40.0 36 33.72317381255263 38.0 33.0 39.0 21.0 40.0 37 33.41620007883468 38.0 33.0 39.0 20.0 40.0 38 33.40680038700661 38.0 33.0 39.0 21.0 40.0 39 32.928123824198664 37.0 32.0 39.0 16.0 40.0 40 33.02036711877161 37.0 32.0 39.0 18.0 40.0 41 32.503191460770786 37.0 32.0 39.0 10.0 40.0 42 32.21684016268611 37.0 31.0 39.0 7.0 40.0 43 32.140701986992276 36.0 31.0 39.0 6.0 40.0 44 31.95532178883056 36.0 31.0 39.0 4.0 40.0 45 31.72094538906706 36.0 31.0 39.0 4.0 40.0 46 31.242732427928978 36.0 30.0 39.0 4.0 40.0 47 31.044425133929373 36.0 30.0 39.0 4.0 40.0 48 30.818557056599715 35.0 29.0 39.0 4.0 40.0 49 30.560194309569454 35.0 29.0 39.0 4.0 40.0 50 30.200976922939102 35.0 29.0 39.0 4.0 40.0 51 29.64767840825614 35.0 28.0 39.0 4.0 40.0 52 29.498542006342607 35.0 27.0 39.0 4.0 40.0 53 29.214770752333685 35.0 27.0 39.0 4.0 40.0 54 28.906995234085247 35.0 26.0 39.0 4.0 40.0 55 28.6036586458352 35.0 25.0 39.0 4.0 40.0 56 27.906789188898642 34.0 21.0 38.0 4.0 40.0 57 27.46565988210632 34.0 20.0 38.0 4.0 39.0 58 27.13516788203465 33.0 18.0 38.0 4.0 39.0 59 26.762951731675415 33.0 15.0 37.0 4.0 39.0 60 26.452233350653074 33.0 4.0 37.0 4.0 39.0 61 26.021648182323116 33.0 4.0 37.0 4.0 39.0 62 25.703299410531596 33.0 4.0 37.0 4.0 39.0 63 25.434063300664718 33.0 4.0 37.0 4.0 39.0 64 25.365817551466503 33.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.19483497867187793 2 2 0.06868412812374203 2 3 -0.009904437083726236 2 4 0.173047253193225 2 5 0.16391634187417736 2 6 0.1848341462799894 2 7 0.2123220291884138 2 8 0.6275749167409259 2 9 0.25097236054120486 2 10 0.6266192709789138 2 11 0.012051833363173614 2 12 0.2809117922637583 2 13 0.07349273011909219 2 14 0.0428286611078974 2 15 0.1047864005636896 2 16 0.07261031322808975 2 17 0.3808032018062164 2 18 -0.12185307158664216 2 19 -0.05606068437953837 2 20 0.15014050817934077 2 21 -0.17028493762843055 2 22 -0.5542472226671009 2 23 -0.2536871010548367 2 24 -0.2559252261137601 2 25 -0.14972243465151536 2 26 0.2111460781823027 2 27 0.3906192331515186 2 28 0.4609321200964729 2 29 0.5275996973232822 2 30 0.40648726400852553 2 31 0.6219790014685458 2 32 0.40323965614631163 2 33 0.1798451332285964 2 34 0.6125547780418401 2 35 0.26043525905731 2 36 -0.5990311628522136 2 37 -0.5857550543042294 2 38 -0.8235964144240171 2 39 -5.0728726999686415 2 40 -1.353850289249074 2 41 -1.1910333978218368 2 42 -1.1230660050243912 2 43 -1.434340423915863 2 44 -0.6996596237483281 2 45 -1.2412785017753016 2 46 -0.08204049833141269 2 47 -0.4737800346784553 2 48 -0.12626244134719755 2 49 -0.24056780663548594 2 50 0.2946189367147518 2 51 0.4405252615407633 2 52 -0.3827130157258054 2 53 -0.09536745094068522 2 54 -0.18386299793511895 2 55 -0.2072517093180899 2 56 -0.09024618285118535 2 57 0.04869849859720787 2 58 0.041668137923263515 2 59 0.2509323824951828 2 60 0.4023698911005127 2 61 0.06223112270829745 2 62 0.01802460615038015 2 63 -0.14618462800809695 2 64 -0.10755351377767397 3 1 -0.13381116725970088 3 2 0.26633910540230943 3 3 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4 59 -0.3947887941274111 4 60 0.0054212714355834635 4 61 -0.05933957370412202 4 62 -0.1540650617939754 4 63 0.20552087179809675 4 64 0.3350796745594664 5 1 -0.1655525514313041 5 2 -0.05303213974484322 5 3 0.09113729862693276 5 4 0.1190990417087292 5 5 0.046607842282917034 5 6 0.01989081758640765 5 7 0.09493620032913697 5 8 0.33481735504321364 5 9 0.14406464749907144 5 10 -0.10083372387316558 5 11 -2.645141997970647 5 12 -2.2116590744868603 5 13 -2.388952863217334 5 14 -1.8917507781444414 5 15 -1.865557825149395 5 16 -19.028210481716783 5 17 -3.2854712950011873 5 18 -3.4624774501907396 5 19 -3.737082977302805 5 20 -3.219112270150788 5 21 -2.9355906091778863 5 22 -3.0237353029127902 5 23 -3.178114312119547 5 24 -3.2106544632052945 5 25 -3.077535562951823 5 26 0.10798331112015092 5 27 0.7252008818115101 5 28 0.5799970987756922 5 29 0.5092174256103306 5 30 0.4752329832811881 5 31 -0.4053016595862289 5 32 -0.05512958006146107 5 33 -0.3626859641841875 5 34 -0.24223742716794305 5 35 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-27.744052147112814 120 45 -27.523988782149132 120 46 -27.07503115253702 120 47 -26.852284707575652 120 48 -26.637944684337853 120 49 -26.41813790009343 120 50 -26.058184801603005 120 51 -25.531437355281668 120 52 -25.37570366993141 120 53 -25.090694553744424 120 54 -24.79367608204727 120 55 -24.459588157916222 120 56 -23.77248917350539 120 57 -23.353170660281297 120 58 -23.01907952562814 120 59 -22.622899393205753 120 60 -22.310246931329395 120 61 -21.898049251123478 120 62 -21.596461375158032 120 63 -21.37282014202679 120 64 -21.257086224058046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 667.0 5 68.0 6 144.0 7 296.0 8 596.0 9 457.0 10 753.0 11 913.0 12 1083.0 13 1548.0 14 1764.0 15 2124.0 16 2746.0 17 3014.0 18 3283.0 19 3889.0 20 4330.0 21 5616.0 22 6793.0 23 6170.0 24 6854.0 25 7287.0 26 7203.0 27 8460.0 28 9813.0 29 11547.0 30 13379.0 31 15752.0 32 18700.0 33 23481.0 34 29499.0 35 36394.0 36 46411.0 37 57017.0 38 62533.0 39 45843.0 40 77.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.41971274549107 0.3970857978557815 0.08174637124498603 0.10145508540816074 8 99.18138038136514 0.49121659021286795 0.18756764872635515 0.13983537969563398 9 98.42800960349739 0.9164531560747495 0.267634780427499 0.38790246000035833 10 54.610708974611654 28.03804669163098 6.179339938723953 11.171904395033415 11 33.70563309623206 29.42414849587014 17.645306648988587 19.224911758909215 12 39.01062476483973 17.36087470660957 23.225099887123072 20.403400641427623 13 27.124281081468478 16.736692168491214 28.269175640083848 27.86985110995646 14 25.16125275473456 20.754573307294 30.35135183559386 23.732822102377582 15 29.722181752978226 19.14805392744674 28.737098093711825 22.39266622586321 16 30.50439294082859 21.33992535808852 23.905573053642716 24.25010864744018 17 31.821097583951623 20.71898298089949 21.86375748006065 25.596161955088238 18 30.44138462365399 23.200016125275475 22.862953075448374 23.495646175622166 19 28.53681042051135 23.786573020622438 22.818160643577663 24.858455915288552 20 26.65452419197656 25.9734702610184 23.172635969952974 24.199369577052067 21 26.563927758765875 22.267213731567914 27.472318277103902 23.696540232562306 22 27.048357909447617 20.809220074176267 27.78071417053375 24.361707845842368 23 29.266703097844587 23.16463010409761 25.71555909913461 21.85310769892319 24 28.79098383603554 25.100842801694114 23.46787252484395 22.6403008374264 25 28.370383650053782 20.325385442811044 24.968402653280748 26.335828253854427 26 25.5394410244003 19.424581305949083 27.274478704624677 27.761498965025943 27 22.40693608406613 18.70502789785301 29.387459208087012 29.500576809993852 28 23.555473799209906 23.06620693677667 27.300462795752956 26.077856468260467 29 26.59777492223889 22.045133959907336 27.847783331875664 23.50930778597811 30 24.9268395587034 21.072675697268377 32.36698713632977 21.633497607698455 31 25.857857992681886 20.310907580757405 33.037915011111856 20.793319415448853 32 28.58918415650555 18.216653392710917 33.90355255925606 19.290609891527478 33 27.645012121756306 18.46031247194038 34.043952590464215 19.850722815839095 34 22.013148487069607 21.223744620766873 39.33011965842713 17.43298723373639 35 20.977574789995217 26.13692244474533 36.73607321930989 16.149429545949562 36 23.395184091409668 24.99651639989032 32.672348306071356 18.935951202628658 37 23.48023701199446 25.032593644913593 33.77668183207754 17.710487511014403 38 25.23805992120481 23.43022040629193 34.00666590253758 17.32505376996568 39 24.231162985780067 21.731061511471104 32.95592407015708 21.081851432591744 40 23.507169095331655 20.931555259809453 34.68929424089419 20.871981403964696 41 19.778025166143777 22.656501253809218 35.45974878835925 22.10572479168775 42 19.45667738362197 20.67201613847387 36.1521627536271 23.719143724277057 43 20.13799779383625 19.016231061885144 38.35708336147318 22.48868778280543 44 22.18269531504895 19.018142625381675 36.26927499251839 22.529887067050986 45 22.115250422059653 20.293528418683174 34.917501406865505 22.673719752391673 46 20.836494539461984 21.92364927384187 34.47466945228023 22.76518673441591 47 19.667609984308058 21.94327936835991 36.9010552099726 21.48805543735943 48 21.50036580561652 22.759412459902077 36.528786088131014 19.211435646350385 49 24.06751955473299 21.68672799895528 34.82836415708631 19.41738828922542 50 20.20196328145251 21.971170643194444 34.539327615548466 23.287538459804573 51 18.798931502653225 24.518004991125885 36.20705965026082 20.47600385596007 52 19.947737153927598 24.15737424252664 35.07873217544097 20.816156428104797 53 21.526574446079348 22.2063989580681 35.86012334634997 20.40690324950258 54 20.48429466816263 22.351316400142387 34.983170444868 22.181218486826985 55 21.705835158792404 22.501825459069153 32.77893465307263 23.013404729065815 56 20.83216877789298 23.721092728666097 31.444574674300142 24.002163819140783 57 21.839064915863162 26.362349610757253 30.97218277955833 20.82640269382125 58 21.37875046510841 28.259197871212887 29.991543483408318 20.370508180270384 59 22.718272314360846 28.490079248738198 29.699332903336384 19.09231553356457 60 25.322178904050247 25.810862031622268 29.058145621254784 19.808813443072705 61 24.22179646117435 24.53285247379691 29.477290657049476 21.768060407979263 62 21.296310909004827 23.877524794217575 29.95957623813271 24.86658805864489 63 20.78415457630176 25.284588589401064 28.801334731193705 25.129922103103475 64 21.590493683968244 24.37189444848113 28.847499086104726 25.190112781445894 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.0 3 2.0 4 6.0 5 20.0 6 30.0 7 212.0 8 394.0 9 400.0 10 515.0 11 624.0 12 790.0 13 1893.0 14 2830.0 15 3723.0 16 4986.5 17 5357.0 18 6121.5 19 6886.0 20 7530.5 21 9214.0 22 10253.0 23 10781.0 24 11972.5 25 12636.0 26 12254.0 27 10514.5 28 9157.0 29 9125.0 30 8039.0 31 6985.0 32 6745.0 33 6505.0 34 6217.0 35 6012.5 36 6096.0 37 6261.0 38 6626.5 39 6827.0 40 6731.0 41 7091.5 42 7548.0 43 7743.0 44 7938.0 45 9224.0 46 10575.0 47 10640.0 48 11161.5 49 11894.0 50 12105.0 51 12621.5 52 14922.5 53 16707.0 54 18325.0 55 20597.5 56 21252.0 57 24780.5 58 28309.0 59 29108.5 60 29508.5 61 29109.0 62 25015.0 63 19212.0 64 17503.0 65 16067.0 66 12422.5 67 10214.0 68 8991.0 69 7768.0 70 6649.5 71 4930.5 72 4330.0 73 3441.5 74 2148.5 75 1744.0 76 1373.5 77 825.0 78 647.0 79 509.0 80 306.0 81 241.0 82 183.0 83 125.0 84 96.0 85 51.5 86 36.0 87 24.0 88 9.0 89 6.0 90 3.5 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.239621593535556E-4 8 0.05934997222869224 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.07816279361439092 16 0.023739988891476896 17 0.14602332789851827 18 0.0 19 0.0 20 0.03068281583143712 21 0.0 22 0.0 23 0.0 24 0.0038073567090104454 25 0.05912601006933868 26 0.025979610485012454 27 0.21276405138587784 28 0.16461218712486336 29 0.13146578754053714 30 0.2033576406930285 31 0.2315768727715765 32 0.17222690054288428 33 0.22933725117804094 34 0.38924623295647964 35 0.25912421837206384 36 0.3498288929102539 37 0.3655062440650028 38 0.3478132334760719 39 0.6812928887535162 40 0.37580850339526634 41 0.4163456542382599 42 0.5258631501621486 43 0.5137691935570566 44 0.4660652536147492 45 0.5048107071829144 46 0.5189203232221884 47 0.5211599448157239 48 0.5104097611667533 49 0.5298944690305126 50 0.6403078135918155 51 0.5634887929335459 52 0.5809578413631232 53 0.6076093383261965 54 0.591708025012094 55 0.6226148030028846 56 0.6369483812015122 57 0.6320212136957339 58 0.6851002454625267 59 0.6920430724024869 60 0.732804185404834 61 0.6391880027950478 62 0.6613602565710497 63 0.6649436511207066 64 0.7493773851969971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 446504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.15799857040079 #Duplication Level Percentage of deduplicated Percentage of total 1 88.20367493769099 53.06156550805711 2 4.748342588981205 5.713015733594092 3 2.060860879249198 3.719317974830045 4 1.2163529274424274 2.9269343068073748 5 0.7948178132027861 2.3907324435191146 6 0.5629786893024862 2.0320602711735045 7 0.41226530849508974 1.7360739087351418 8 0.3224895947965595 1.5520262866192445 9 0.25471256326182024 1.3790698214910935 >10 1.295332617092025 13.704873700421516 >50 0.0771035173160558 3.1998889739529144 >100 0.0457280598313393 5.358448596915685 >500 0.0030040331276062313 1.199958360757256 >1k 0.002336470210360402 2.026034113125956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1972 0.4416533782452117 No Hit TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 1906 0.42687187572787705 No Hit TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 1650 0.3695375629333668 No Hit TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1237 0.2770411911203483 No Hit TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 1147 0.2568845967785283 No Hit TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 1130 0.2530772400695179 No Hit TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1048 0.23471234300252627 No Hit TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 849 0.19014387329116872 No Hit TAGAGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG 823 0.18432085714797627 No Hit TAGAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC 689 0.15430992779459982 No Hit TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 660 0.14781502517334671 No Hit TAGAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 657 0.14714313869528603 No Hit TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 625 0.13997634959597224 No Hit TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 610 0.13661691720566893 No Hit TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 537 0.12026767957285936 No Hit TAGAGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC 526 0.11780409581997026 No Hit TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 495 0.11086126888001005 No Hit TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 478 0.10705391217099959 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.006046978302546002 0.0 0.0 0.0 0.0 5 0.006046978302546002 0.0 0.0 0.0 0.0 6 0.01075018364897067 0.0 0.0 0.0 0.0 7 0.010974145808324226 0.0 0.0 0.0 0.0 8 0.011422070127031336 0.0 0.0 0.0 0.0 9 0.01231791876444556 0.0 0.0 0.0 0.0 10 0.02239621593535556 0.0 0.0 0.0 0.0 11 0.03471413469980112 0.0 0.0 0.0 0.0 12 0.036057907655922455 0.0 0.0 0.0 0.0 13 0.038297529249458014 0.0 0.0 0.0 0.0 14 0.043896583233296906 0.0 0.0 0.0 0.0 15 0.045912242667478904 0.0 0.0 0.0 0.0 16 0.04882375073907513 0.0 0.0 0.0 0.0 17 0.051511296651317796 0.0 0.0 0.0 0.0 18 0.05487072904162113 0.0 0.0 0.0 0.0 19 0.05890204790998513 0.0 0.0 0.0 0.0 20 0.06629279916865247 0.0 0.0 0.0 0.0 21 0.0696522315589558 0.0 0.0 0.0 0.0 22 0.07547524770214825 0.0 0.0 0.0 0.0 23 0.08062637736728002 0.0 0.0 0.0 0.0 24 0.08712127998853314 0.0 0.0 0.0 0.0 25 0.08868901510400802 0.0 0.0 0.0 0.0 26 0.09294429613172558 0.0 0.0 0.0 0.0 27 0.09809542579685736 0.0 0.0 0.0 0.0 28 0.11041334456130292 0.0 0.0 0.0 0.0 29 0.11332485263289914 0.0 0.0 0.0 0.0 30 0.12004371741350581 0.0 0.0 0.0 0.0 31 0.1231791876444556 0.0 0.0 0.0 0.0 32 0.12698654435346604 0.0 0.0 0.0 0.0 33 0.1307939010624765 0.0 0.0 0.0 0.0 34 0.13348144697471914 0.0 0.0 0.0 0.0 35 0.15050257108558937 0.0 0.0 0.0 0.0 36 0.1554297385913676 0.0 0.0 0.0 0.0 37 0.1590131331410245 0.0 0.0 0.0 0.0 38 0.1634923763280956 0.0 0.0 0.0 0.0 39 0.16797161951516673 0.0 0.0 0.0 0.0 40 0.17133105190547004 0.0 0.0 0.0 0.0 41 0.1771540680486625 0.0 0.0 0.0 0.0 42 0.18208123555444072 2.2396215935355563E-4 0.0 0.0 0.0 43 0.18880010033504738 2.2396215935355563E-4 0.0 0.0 0.0 44 0.19619085159371472 2.2396215935355563E-4 0.0 0.0 0.0 45 0.2008940569401394 2.2396215935355563E-4 0.0 0.0 0.0 46 0.20626914876462474 4.4792431870711126E-4 0.0 0.0 0.0 47 0.21455574866070629 4.4792431870711126E-4 0.0 0.0 0.0 48 0.22149857560066652 4.4792431870711126E-4 0.0 0.0 0.0 49 0.22799347822191962 4.4792431870711126E-4 0.0 0.0 0.0 50 0.2331446078870514 4.4792431870711126E-4 0.0 0.0 0.0 51 0.23851969971153675 4.4792431870711126E-4 0.0 0.0 0.0 52 0.24501460233278985 4.4792431870711126E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATAGT 35 1.6088961E-8 58.13261 46 CGAAGTA 15 0.00465354 58.099854 44 AACCGAC 15 0.004666012 58.060593 30 GCAATTC 15 0.00467434 58.034447 27 GTCATTC 15 0.004754013 57.787243 10 AGAGGGG 24810 0.0 55.728764 4 GAGAGGG 44945 0.0 55.712074 3 TAGAGAG 46110 0.0 55.56933 1 AGAGAGG 45960 0.0 55.248077 2 GAGGGAT 2500 0.0 55.20129 5 GAGGGGG 10260 0.0 54.98428 5 AGAGGGA 13015 0.0 54.88035 4 AGAGGGT 2750 0.0 54.802345 4 AGGGTAC 385 0.0 54.74235 6 AGGGGGG 5370 0.0 54.139782 6 AGGGAGT 1300 0.0 53.9665 6 AGGGGCT 2735 0.0 53.941734 6 GAGGGGA 6040 0.0 53.870163 5 AGGGATC 665 0.0 53.834587 6 GAGGGGT 3910 0.0 53.82848 5 >>END_MODULE