FastQCFastQC Report
Fri 17 Jun 2016
SRR1526452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407273
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT26590.6528790270899372No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC19110.469218435791226No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC17950.4407363120069339No Hit
TAGAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC16980.41691936367006893No Hit
TAGAGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA16600.4075890127752147No Hit
TAGAGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14970.3675667181472869No Hit
TAGAGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.3344194189155677No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC10800.26517839385375414No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC8960.2199998526786701No Hit
TAGAGAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT8880.2180355682797534No Hit
TAGAGAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA8360.20526771968679489No Hit
TAGAGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.19937486649004477No Hit
TAGAGAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT7570.18587041124749246No Hit
TAGAGAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG6920.1699106005062943No Hit
TAGAGAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC6460.1586159652125233No Hit
TAGAGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5760.1414284767220022No Hit
TAGAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5590.1372543723743042No Hit
TAGAGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.12939723477863743No Hit
TAGAGAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC5130.1259597370805332No Hit
TAGAGAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG4990.12252223938242898No Hit
TAGAGAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA4920.12080349053337687No Hit
TAGAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4830.11859367058459559No Hit
TAGAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC4790.11761152838513725No Hit
TAGAGAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC4790.11761152838513725No Hit
TAGAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA4600.11294635293771008No Hit
TAGAGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.10950885523960588No Hit
TAGAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT4320.10607135754150164No Hit
TAGAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4110.10091511099434532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGGT150.00828249250.21938344
GCATCGT150.00828655250.2131643
ATAACCC252.0421974E-550.17585442
ACCTTAC150.00835986450.101441
TAGGTAA150.00849138949.90392730
AATGCGT150.00849138949.90392730
AACGTAT204.2256608E-449.8916428
TCACCGT150.00851207849.87321526
ATAGTTA150.00851207849.87321527
AAACGTA204.2333693E-449.8732127
GCCAATT204.2333693E-449.8732126
CGATATT204.2824497E-449.75684720
CGAATAT150.00859103849.75684419
TGCTAAT150.00859103849.75684420
ATTGTCG150.00859938149.7446312
GGATAGC150.00859938149.744638
AGATTAT150.00859938149.7446315
CCGAATA150.00859938149.7446318
ATTATCC150.00859938149.7446314
TACGATC204.287642E-449.74462512
Produced by FastQC (version 0.11.5)