FastQCFastQC Report
Fri 17 Jun 2016
SRR1526451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99290
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15941.6053983281297208No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6770.6818410716084198No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.6103333669050257No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.37264578507402557No Hit
CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2900.2920737234363984No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.28300936650216535No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.24876624030617384No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.21653741565112297No Hit
CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.20042300332359753No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAA1890.19035149561889414No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA1720.17322993252089838No Hit
CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.17020848020948737No Hit
CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1650.166179877127606No Hit
CCAGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.16114412327525432No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.1581226709638433No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA1490.15006546480008057No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAA1440.14502971094772887No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA1350.13596535401349583No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.1329439017020848No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAA1250.1258938463087924No Hit
CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1190.11985094168597038No Hit
CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.11582233860408904No Hit
CCAGACGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.1047436801289153No Hit
CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1010.10172222781750428No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAAG201.9767604E-458.14524558
GTGATTT201.9767604E-458.14524552
CTATATA150.00463781658.11583745
TGCAGGA150.00463781658.11583748
GGTGATT201.981715E-458.11583351
TTCGTAT258.510144E-658.11583350
ATTCGTA258.510144E-658.11583349
CGGTGAT201.981715E-458.11583350
TGGGTAA150.004656431758.057133
TCCGGTA201.9966383E-458.0277830
CCGGTAC150.004665760358.0277831
TCGCATT150.0046844657.96922328
TGCATTC258.690511E-657.9107925
TTGCATT258.690511E-657.9107924
GTAAAGA202.016675E-457.91078622
TGATGGA258.716534E-657.88161515
GGATGTA407.203198E-1057.8816158
ACTGTAT202.021709E-457.88161510
CGGAGTG202.021709E-457.8816156
GGGCATC258.716534E-657.8816157
Produced by FastQC (version 0.11.5)