FastQCFastQC Report
Fri 17 Jun 2016
SRR1526450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96770
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14141.4611966518549135No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.5724914746305673No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.4619200165340498No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.31001343391546965No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.23974372222796322No Hit
CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2230.2304433192104991No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.20460886638421No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1680.17360752299266302No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA1630.1684406324274052No Hit
CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.15810685129688953No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAA1430.1477730701663739No Hit
CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1370.14157280148806448No Hit
CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.13847266714890977No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA1250.1291722641314457No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.12813888601839413No Hit
CCAGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.12193861734008474No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1170.12090523922703317No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAA1130.1167717267748269No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA1020.10540456753125968No Hit
CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.102304433192105No Hit
CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG970.10023767696600186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACCA201.9532318E-458.28444736
TCCCACC201.9684114E-458.19333335
GCTCAGG150.00462253358.16301758
TCGCTCC201.978583E-458.13274831
ATCGCTC201.9887966E-458.07228530
CACGTAT150.00465121558.0722828
ACATCGC150.00465121558.0722828
CACATTC150.00466080558.042127
CGCTCCC201.993919E-458.042132
AAGACGT201.993919E-458.042126
AGACGTA201.993919E-458.042127
TAACATC150.00466080558.042126
GCCATGA407.039489E-1058.042125
CCGGGCT150.00466080558.042132
GTAACAT150.00466080558.042125
CGCGATC303.7067002E-758.042125
CCATGAT351.610897E-858.04209526
CCGCACA150.004689663657.95173624
TGTAACA150.004689663657.95173624
TTGTAAC150.004699312657.9216823
Produced by FastQC (version 0.11.5)