##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526450_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96770 Sequences flagged as poor quality 0 Sequence length 64 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.3104991216286 39.0 38.0 40.0 35.0 40.0 2 38.10181874547897 39.0 38.0 40.0 35.0 40.0 3 37.67328717577762 39.0 38.0 40.0 33.0 40.0 4 37.575457269815026 39.0 38.0 40.0 33.0 40.0 5 37.64460059935931 39.0 38.0 40.0 33.0 40.0 6 37.92964761806345 39.0 38.0 40.0 35.0 40.0 7 38.144631600702695 39.0 38.0 40.0 35.0 40.0 8 37.95644311253488 39.0 38.0 40.0 35.0 40.0 9 38.03454583031932 39.0 38.0 40.0 35.0 40.0 10 37.05956391443629 39.0 37.0 40.0 33.0 40.0 11 37.13906169267335 39.0 37.0 40.0 33.0 40.0 12 36.92484240983776 39.0 36.0 40.0 32.0 40.0 13 36.804350521855945 39.0 36.0 40.0 32.0 40.0 14 36.78267024904412 39.0 36.0 40.0 32.0 40.0 15 36.45952257931177 38.0 36.0 39.0 31.0 40.0 16 36.13705693913403 38.0 35.0 39.0 30.0 40.0 17 35.94943680892839 38.0 35.0 39.0 29.0 40.0 18 36.27363852433606 38.0 35.0 39.0 30.0 40.0 19 36.03660225276429 38.0 35.0 39.0 30.0 40.0 20 36.24432158726878 38.0 35.0 39.0 30.0 40.0 21 36.14087010437119 38.0 35.0 39.0 30.0 40.0 22 35.74126278805415 38.0 35.0 39.0 29.0 40.0 23 35.62717784437326 38.0 35.0 39.0 29.0 40.0 24 35.4938720677896 38.0 35.0 39.0 29.0 40.0 25 35.521122248630775 38.0 35.0 39.0 29.0 40.0 26 35.78476800661362 38.0 35.0 39.0 29.0 40.0 27 35.3788674175881 38.0 35.0 39.0 27.0 40.0 28 35.257972512142196 38.0 35.0 39.0 27.0 40.0 29 35.30559057559161 38.0 35.0 39.0 27.0 40.0 30 35.26690089903896 38.0 35.0 39.0 27.0 40.0 31 35.2937687299783 39.0 35.0 39.0 28.0 40.0 32 35.30560090937274 39.0 35.0 39.0 27.0 40.0 33 35.40998243257208 39.0 35.0 39.0 28.0 40.0 34 34.90803968171954 39.0 35.0 39.0 25.0 40.0 35 34.99599049292136 39.0 35.0 39.0 27.0 40.0 36 34.87195411801178 39.0 35.0 39.0 26.0 40.0 37 34.79606282938927 39.0 35.0 39.0 26.0 40.0 38 34.80397850573525 39.0 35.0 39.0 26.0 40.0 39 34.403885501705076 39.0 35.0 39.0 23.0 40.0 40 34.57569494678103 39.0 35.0 39.0 25.0 40.0 41 34.68116151699907 39.0 35.0 39.0 26.0 40.0 42 34.42304433192105 39.0 35.0 39.0 23.0 40.0 43 34.44963315076987 39.0 35.0 39.0 24.0 40.0 44 34.23418414797975 39.0 34.0 39.0 23.0 40.0 45 34.023385346698355 39.0 34.0 39.0 22.0 40.0 46 33.71971685439702 39.0 34.0 39.0 18.0 40.0 47 33.63364679136096 39.0 34.0 39.0 17.0 40.0 48 33.529595949157795 39.0 34.0 39.0 16.0 40.0 49 33.26508215355999 38.0 33.0 39.0 13.0 40.0 50 32.84870311046812 38.0 33.0 39.0 6.0 40.0 51 32.48259791257621 38.0 33.0 39.0 4.0 40.0 52 32.33506251937584 38.0 32.0 39.0 4.0 40.0 53 32.02544176914333 38.0 32.0 39.0 4.0 40.0 54 31.642823189004858 37.0 32.0 39.0 4.0 40.0 55 31.10603492818022 37.0 31.0 39.0 4.0 40.0 56 30.29977265681513 37.0 30.0 39.0 4.0 40.0 57 29.817670765733183 36.0 28.0 39.0 4.0 40.0 58 29.461899348971787 35.0 26.0 39.0 4.0 40.0 59 29.022289965898523 35.0 24.0 39.0 4.0 40.0 60 28.49720987909476 35.0 22.0 39.0 4.0 40.0 61 27.87861940684096 35.0 4.0 39.0 4.0 40.0 62 27.656267438255657 35.0 4.0 39.0 4.0 40.0 63 27.200072336467915 35.0 4.0 39.0 4.0 40.0 64 27.183600289345872 35.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.1652366115419568 2 2 0.42815850398430655 2 3 0.19091210557884608 2 4 0.3993901483973872 2 5 0.31894909016145334 2 6 0.6689053920426247 2 7 0.40151094362187223 2 8 0.5245151196954367 2 9 -0.05480051498827265 2 10 0.542551686747295 2 11 0.2842596309321195 2 12 0.2921028760324944 2 13 0.46458044425899914 2 14 0.37679468381625014 2 15 0.19204534638350168 2 16 -0.784918288678945 2 17 -0.07411222867215628 2 18 -0.39195158002598873 2 19 -0.5392921989566659 2 20 -0.11763934432475764 2 21 -0.07067525408702835 2 22 -0.5876802112319979 2 23 -0.19539818785013807 2 24 0.48134368461659705 2 25 0.15668546596014465 2 26 0.1412705013818396 2 27 1.0534912816215325 2 28 0.6911060818940982 2 29 0.9657164299325771 2 30 1.1486189821555257 2 31 0.9204034771178584 2 32 -0.05749343296891141 2 33 -0.010804580666203378 2 34 0.3386059098738272 2 35 0.09580147948177853 2 36 -0.09898228347284288 2 37 -0.03252086984028324 2 38 -0.4600562407077078 2 39 -5.280267700000792 2 40 -0.9692190891937216 2 41 -0.21883369111670703 2 42 -0.3090969235773713 2 43 -0.7696509196425865 2 44 -0.4870997331257101 2 45 -0.20758418470257567 2 46 -0.881369934927271 2 47 -0.4700097363924698 2 48 -0.8666735156476975 2 49 -1.1376274516425937 2 50 -0.6433777551174273 2 51 -1.6983083206267295 2 52 -1.9455471089868546 2 53 -2.217427412058008 2 54 -2.4594771656351995 2 55 -1.9630350023699137 2 56 -2.796272441329979 2 57 -3.1877171989687625 2 58 -3.305648903654987 2 59 -2.646561671199077 2 60 -2.7081516646240686 2 61 -3.111796004294174 2 62 -3.5804355340742653 2 63 -3.554907110922688 2 64 -3.760052926413273 3 1 0.24258517063626073 3 2 1.085584246558561 3 3 0.48566458082637354 3 4 0.9209743068132283 3 5 0.7922164168941208 3 6 0.8887073722406456 3 7 0.8715109436218711 3 8 0.9433270008835493 3 9 0.6697539404572694 3 10 1.1915615877373895 3 11 0.18406161113014008 3 12 0.5301226780126882 3 13 0.6526992561401883 3 14 1.134022406588521 3 15 1.1449166335122172 3 16 0.7844876519151143 3 17 0.23054123667438375 3 18 -0.25719910477846497 3 19 0.32130186044927456 3 20 0.608400259635637 3 21 0.36397821125950713 3 22 0.40915147193631896 3 23 -0.0055962076521183235 3 24 0.3562941796661008 3 25 0.38846764417796464 3 26 1.2757259469263929 3 27 1.7534912816215353 3 28 2.1059575670426085 3 29 2.0034392022098046 3 30 2.0680249227495864 3 31 2.4278292196921143 3 32 2.080922408615244 3 33 2.0794924490367634 3 34 2.6280118504678853 3 35 2.162336132947125 3 36 -2.6444278280273004 3 37 -2.9484614638996938 3 38 -3.130254260509691 3 39 -21.8121488881196 3 40 -3.5873379010749105 3 41 -3.968338641611755 3 42 -4.301770190904104 3 43 -4.176878642414859 3 44 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0.8402625830474335 4 23 0.6829896509337416 4 24 0.5923547857267124 4 25 1.0188716845820096 4 26 1.8068370580375088 4 27 2.467632695762944 4 28 2.2160585771436203 4 29 1.6883886971592972 4 30 2.1474188621435246 4 31 0.5313645732274708 4 32 -0.010188702495867119 4 33 -0.06070957116525477 4 34 0.5697290221850579 4 35 -5.002108311497317 4 36 -0.07968035327982648 4 37 -0.47886550430373376 4 38 -0.6220724423278696 4 39 -0.20325999923071691 4 40 -0.12703487077187958 4 41 1.2064088331357183 4 42 1.2107550616211498 4 43 1.0541314585952435 4 44 0.6207110479523976 4 45 0.25656222993888633 4 46 0.4211603180980319 4 47 0.37427469205037056 4 48 0.5035635080546683 4 49 -0.30244393329076047 4 50 0.2136079434524305 4 51 0.5234138515904903 4 52 0.27947539326337534 4 53 0.501444475130711 4 54 0.4175105331346778 4 55 0.8725485559859187 4 56 0.2182290088150367 4 57 0.13161473422455572 4 58 0.2465063118665647 4 59 0.08231121608965353 4 60 -0.12099294875248034 4 61 0.47556273157941575 4 62 0.6298854980289441 4 63 1.181166496438049 4 64 0.6982929081701847 5 1 0.04238108900361226 5 2 0.7161964914565218 5 3 0.18505233592841108 5 4 0.404239612935676 5 5 -0.24247746065690023 5 6 0.627482882444724 5 7 0.7178374742341163 5 8 0.7147555723121215 5 9 0.4387335322940018 5 10 0.49870444488024646 5 11 -1.1143057358086352 5 12 -1.3057956893342464 5 13 -1.3677089071251203 5 14 -1.2235286138196386 5 15 -0.7073282644469714 5 16 -19.17449193992162 5 17 -2.1619077429174567 5 18 -1.9182195129417323 5 19 -3.1191063028160357 5 20 -2.6093548424051747 5 21 -2.913572809148654 5 22 -2.8218689362269487 5 23 -2.6960043709174286 5 24 -2.7381956162522627 5 25 -2.378675212964886 5 26 0.3691953346814998 5 27 1.3800218938664273 5 28 1.5120800160222032 5 29 1.754663692005721 5 30 1.116188188055709 5 31 0.5043598319370091 5 32 0.22337138820708446 5 33 0.01051285720003392 5 34 0.20413429944748174 5 35 -5.28215366297124 5 36 -0.29932578721097514 5 37 -0.28540023940989556 5 38 -0.5320909952035677 5 39 -0.2962305207726672 5 40 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-30.60198814953211 120 35 -30.557663867052874 120 36 -30.5544278280273 120 37 -30.438461463899692 120 38 -30.44025426050969 120 39 -30.092148888119603 120 40 -30.15733790107491 120 41 -30.268338641611756 120 42 -30.041770190904103 120 43 -30.04687864241486 120 44 -29.823733396492045 120 45 -29.68283170945505 120 46 -29.356617459679747 120 47 -29.262088944313263 120 48 -29.193406188915027 120 49 -28.979211610058435 120 50 -28.564169834325348 120 51 -28.173555845379205 120 52 -28.08416097037299 120 53 -27.801585827899594 120 54 -27.37036825474411 120 55 -26.824421140983777 120 56 -26.014094223508195 120 57 -25.49464789203807 120 58 -25.13733207197182 120 59 -24.725769591991156 120 60 -24.272508100267633 120 61 -23.686053430036747 120 62 -23.491326623183177 120 63 -22.990550675279124 120 64 -22.948171738294462 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 159.0 5 11.0 6 50.0 7 53.0 8 200.0 9 128.0 10 164.0 11 159.0 12 221.0 13 261.0 14 243.0 15 330.0 16 408.0 17 407.0 18 446.0 19 511.0 20 515.0 21 659.0 22 689.0 23 678.0 24 771.0 25 1047.0 26 1242.0 27 1525.0 28 2045.0 29 2306.0 30 3100.0 31 3660.0 32 4405.0 33 5446.0 34 6380.0 35 7262.0 36 8604.0 37 11887.0 38 17510.0 39 13254.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.24356722124625 0.5435568874651235 0.12813888601839413 0.08473700527022839 8 98.80158410108467 0.7496561921601472 0.2388559729503366 0.20990373380484123 9 93.1042678516069 4.016740725431435 1.1708174020874238 1.708174020874238 10 25.59264234783507 45.65464503461817 10.25111088147153 18.50160173607523 11 20.083703627157178 38.77234680169474 20.676862664048777 20.467086907099308 12 41.9499844993283 17.964245117288417 25.387000103337808 14.698770280045467 13 23.619923530019634 17.475457269815024 25.87475457269815 33.02986462746719 14 24.71323757362819 29.904929213599257 20.314146946367675 25.067686266404877 15 34.16286550614909 26.420910002999555 19.860158666128815 19.55606582472254 16 25.521183243584044 25.830223977013155 24.813180225527383 23.83541255387541 17 25.83139264089427 31.01381773016612 17.111214614707862 26.043575014231745 18 26.128965588508834 24.59543246874031 20.213909269401675 29.061692673349178 19 24.05290895938824 26.27777203678826 24.999483310943475 24.669835692880024 20 25.809919576588307 28.597862267154582 17.860613202671132 27.731604953585975 21 24.966415211325824 22.523509352071926 26.874031208019016 25.636044228583238 22 23.158003513485586 26.982535909889428 21.8817815438669 27.977679032758086 23 26.894698770280044 32.261031311356824 22.241397127208845 18.602872791154283 24 22.995205026455025 34.53207671957672 24.347924933862434 18.12479332010582 25 23.693869743685184 23.01560222091256 28.51825428828438 24.77227374711788 26 23.587236285829466 23.33605532183207 31.095789876270118 21.980918516068346 27 18.273948779528375 21.376369019274964 34.59555702780481 25.754125173391856 28 17.39818887451488 26.288227684346698 38.582147477360934 17.73143596377749 29 19.945994040721736 21.665494123489488 39.019822877007115 19.368688958781657 30 13.09348793871001 27.338233771611968 42.706284294440415 16.861993995237604 31 15.271951358462033 24.40363361403727 46.71182789016293 13.612587137337767 32 9.597350170789772 23.74702411758617 54.2697443328848 12.38588137873926 33 12.51411229763742 22.951515842025128 50.47282670616384 14.061545154173615 34 13.995124734194283 22.87744411596909 51.865567138633885 11.261864011202738 35 10.69208454206382 26.30232076253626 52.85536676336511 10.150227932034811 36 9.338477014176233 23.913967789772787 53.51502141471964 13.232533781331343 37 9.10307065301932 22.35427101805473 57.03782057264931 11.504837756276638 38 8.448864048776947 23.37539791992866 57.72768278393596 10.448055247358434 39 8.8132453639145 23.370866027015214 56.37604845255687 11.439840156513414 40 8.999356966541518 23.329668734053808 58.49841315936858 9.172561140036093 41 9.379377613597452 24.622552427597512 57.355428500274975 8.642641458530056 42 10.716734185104395 21.893632491949724 57.384439596966864 10.005193725979018 43 11.700237889947331 22.54968160144604 58.382763886436116 7.367316622170512 44 10.884057068095446 22.693290137686127 59.223724378543395 7.198928415675036 45 9.22849990131613 26.04319237121755 57.55035474253898 7.1779529849273365 46 7.803327064348873 25.514074043287167 58.23921197826291 8.443386914101058 47 8.317401604255851 26.427621462117116 58.30701134616184 6.947965587465192 48 10.487331054758515 26.342471872759948 56.60146064264121 6.568736429840327 49 10.313155871412839 26.200569374311662 56.36597884587411 7.1202959084013875 50 9.565705728191688 26.273971463039224 56.31785847997005 7.842464328799034 51 8.238668842304374 26.758265171435404 57.08346757849444 7.919598407765779 52 8.221143350209479 28.024451351997588 54.75980081296588 8.994604484827063 53 9.02786442087752 29.584113121231027 53.81264296111458 7.575379496776876 54 9.168789312262827 29.356968342257105 53.95020013515621 7.524042210323854 55 8.591158210010711 30.68627349022952 52.58067535384839 8.141892945911373 56 9.357643583716431 30.65858174027001 51.789986062862724 8.193788613150833 57 9.14083056858483 31.51501284438019 51.273517696123804 8.070638890911171 58 9.45467790612967 31.705692579873034 50.229992715162865 8.609636798834426 59 9.297630321889082 32.77690474455973 49.54573364276869 8.379731290782503 60 9.657872269537513 32.59167482248089 48.54757095558379 9.20288195239781 61 9.94747516771543 32.91174788080504 48.309324457850124 8.831452493629415 62 9.328117522212281 33.62429513722716 47.05570236584198 9.991884974718575 63 9.952769339603012 35.69868087054221 45.24052265823312 9.108027131621656 64 11.388885996605616 35.2110036338647 44.68716485667579 8.712945512853885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 2.5 6 5.0 7 5.0 8 5.0 9 6.0 10 98.0 11 189.0 12 171.5 13 200.0 14 246.0 15 317.0 16 955.0 17 1522.0 18 2026.0 19 2530.0 20 2765.5 21 3478.5 22 3956.0 23 4693.5 24 5816.0 25 6201.0 26 6571.5 27 7039.0 28 7136.0 29 6853.0 30 5506.5 31 4443.0 32 4635.5 33 4828.0 34 3909.5 35 2726.0 36 2461.0 37 2429.5 38 2376.5 39 2355.0 40 2340.5 41 2229.0 42 2132.0 43 2017.5 44 1903.0 45 1794.5 46 1653.0 47 1620.0 48 1814.0 49 2184.5 50 2361.0 51 2200.5 52 1842.0 53 1644.0 54 1627.5 55 1971.0 56 2331.0 57 2341.0 58 2351.0 59 2100.5 60 1729.0 61 1608.0 62 1573.0 63 1418.0 64 1298.0 65 1128.5 66 759.0 67 559.0 68 482.0 69 405.0 70 332.0 71 221.5 72 184.0 73 155.5 74 103.0 75 79.0 76 63.5 77 42.5 78 37.0 79 31.0 80 16.5 81 8.0 82 8.5 83 9.0 84 5.0 85 1.5 86 2.0 87 1.0 88 1.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.06096930867004237 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.09197065206158933 16 0.019634184147979745 17 0.16017360752299264 18 0.0 19 0.0 20 0.033068099617650094 21 0.0 22 0.0 23 0.0 24 0.002066756226103131 25 0.05476903999173297 26 0.02790120905239227 27 0.17360752299266302 28 0.149839826392477 29 0.11780510488787847 30 0.1860080603492818 31 0.23457683166270538 32 0.1653404980882505 33 0.2304433192104991 34 0.3771830112638214 35 0.2583445282628914 36 0.35238193655058386 37 0.35238193655058386 38 0.34204815542006817 39 0.6985636044228584 40 0.3637490957941511 41 0.4123178671075747 42 0.5166890565257828 43 0.5239227033171437 44 0.47948744445592645 45 0.5197891908649375 46 0.5466570218042782 47 0.5435568874651235 48 0.52805621576935 49 0.5414901312390203 50 0.6344941614136613 51 0.5714580965175157 52 0.5983259274568564 53 0.6096930867004237 54 0.6045261961351659 55 0.6334607833006097 56 0.6448279425441769 57 0.6396610519789191 58 0.7026971168750646 59 0.7037304949881162 60 0.7481657538493335 61 0.6458613206572285 62 0.6737625297096208 63 0.6675622610313113 64 0.7533326444145912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 96770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.39774723571355 #Duplication Level Percentage of deduplicated Percentage of total 1 83.88916055419723 53.18383796631188 2 7.876120619396904 9.98656608453033 3 3.101874490627547 5.899555647411388 4 1.5321923390383048 3.8855017050738865 5 0.8035859820700897 2.5472770486721092 6 0.6405867970660147 2.436705590575592 7 0.4466177669111654 1.9820192208329028 8 0.34881825590872045 1.7691433295442802 9 0.23797881010594948 1.3578588405497571 >10 1.0578647106764465 10.329647618063449 >50 0.03422982885085575 1.4911646171334092 >100 0.02770986145069275 3.0970342048155417 >500 0.0016299918500407497 0.5724914746305673 >1k 0.0016299918500407497 1.4611966518549135 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1414 1.4611966518549135 No Hit CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 554 0.5724914746305673 No Hit CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 447 0.4619200165340498 No Hit CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.31001343391546965 No Hit CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.23974372222796322 No Hit CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 223 0.2304433192104991 No Hit CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.20460886638421 No Hit CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 168 0.17360752299266302 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAAA 163 0.1684406324274052 No Hit CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.15810685129688953 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAAAAAAAAA 143 0.1477730701663739 No Hit CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 137 0.14157280148806448 No Hit CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.13847266714890977 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAAAAAAAAAA 125 0.1291722641314457 No Hit CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.12813888601839413 No Hit CCAGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 0.12193861734008474 No Hit CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.12090523922703317 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAA 113 0.1167717267748269 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAAAAAAAAAA 102 0.10540456753125968 No Hit CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.102304433192105 No Hit CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG 97 0.10023767696600186 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0010333781130515657 0.0 0.0 0.0 0.0 8 0.0031001343391546966 0.0 0.0 0.0 0.0 9 0.0031001343391546966 0.0 0.0 0.0 0.0 10 0.011367159243567222 0.0 0.0 0.0 0.0 11 0.026867830939340705 0.0 0.0 0.0 0.0 12 0.02790120905239227 0.0 0.0 0.0 0.0 13 0.034101477730701664 0.0 0.0 0.0 0.0 14 0.043401880748165755 0.0 0.0 0.0 0.0 15 0.04753539320037201 0.0 0.0 0.0 0.0 16 0.04856877131342358 0.0 0.0 0.0 0.0 17 0.050635527539526715 0.0 0.0 0.0 0.0 18 0.05373566187868141 0.0 0.0 0.0 0.0 19 0.06200268678309393 0.0 0.0 0.0 0.0 20 0.07130308980055802 0.0 0.0 0.0 0.0 21 0.07853673659191898 0.0 0.0 0.0 0.0 22 0.09920429885295029 0.0 0.0 0.0 0.0 23 0.10747132375736282 0.0 0.0 0.0 0.0 24 0.11470497054872378 0.0 0.0 0.0 0.0 25 0.11780510488787847 0.0 0.0 0.0 0.0 26 0.12710550790534256 0.0 0.0 0.0 0.0 27 0.13743928903585823 0.0 0.0 0.0 0.0 28 0.16947401054045674 0.0 0.0 0.0 0.0 29 0.17360752299266302 0.0 0.0 0.0 0.0 30 0.19117495091453962 0.0 0.0 0.0 0.0 31 0.19634184147979747 0.0 0.0 0.0 0.0 32 0.20564224449726154 0.0 0.0 0.0 0.0 33 0.21804278185388035 0.0 0.0 0.0 0.0 34 0.2314766973235507 0.0 0.0 0.0 0.0 35 0.3120801901415728 0.0 0.0 0.0 0.0 36 0.3275808618373463 0.0 0.0 0.0 0.0 37 0.3368812648548104 0.0 0.0 0.0 0.0 38 0.3534153146636354 0.0 0.0 0.0 0.0 39 0.37098274258551206 0.0 0.0 0.0 0.0 40 0.37924976748992456 0.0 0.0 0.0 0.0 41 0.394750439185698 0.0 0.0 0.0 0.0 42 0.40921773276841994 0.0 0.0 0.0 0.0 43 0.4267851606902966 0.0 0.0 0.0 0.0 44 0.4391856980469154 0.0 0.0 0.0 0.0 45 0.4505528572904826 0.0 0.0 0.0 0.0 46 0.46502015087320453 0.0 0.0 0.0 0.0 47 0.4774206882298233 0.0 0.0 0.0 0.0 48 0.4918879818125452 0.0 0.0 0.0 0.0 49 0.5125555440735765 0.0 0.0 0.0 0.0 50 0.5156556784127312 0.0 0.0 0.0 0.0 51 0.5290895938824016 0.0 0.0 0.0 0.0 52 0.544590265578175 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCACCA 20 1.9532318E-4 58.284447 36 TCCCACC 20 1.9684114E-4 58.193333 35 GCTCAGG 15 0.004622533 58.163017 58 TCGCTCC 20 1.978583E-4 58.132748 31 ATCGCTC 20 1.9887966E-4 58.072285 30 CACGTAT 15 0.004651215 58.07228 28 ACATCGC 15 0.004651215 58.07228 28 CACATTC 15 0.004660805 58.0421 27 CGCTCCC 20 1.993919E-4 58.0421 32 AAGACGT 20 1.993919E-4 58.0421 26 AGACGTA 20 1.993919E-4 58.0421 27 TAACATC 15 0.004660805 58.0421 26 GCCATGA 40 7.039489E-10 58.0421 25 CCGGGCT 15 0.004660805 58.0421 32 GTAACAT 15 0.004660805 58.0421 25 CGCGATC 30 3.7067002E-7 58.0421 25 CCATGAT 35 1.610897E-8 58.042095 26 CCGCACA 15 0.0046896636 57.951736 24 TGTAACA 15 0.0046896636 57.951736 24 TTGTAAC 15 0.0046993126 57.92168 23 >>END_MODULE