FastQCFastQC Report
Fri 17 Jun 2016
SRR1526449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84669
Sequences flagged as poor quality0
Sequence length56
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT16741.9771108670233497No Hit
CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA7750.9153291051034027No Hit
CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.6330534197876436No Hit
CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.3862098288629841No Hit
CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.32479419858507835No Hit
CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2670.31534564008078514No Hit
CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.2751892664375391No Hit
CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.2598353588680627No Hit
CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.194876519151047No Hit
CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1550.18306582102068053No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA1550.18306582102068053No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAA1540.18188475120764389No Hit
CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.1629876341990575No Hit
CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.1582633549469109No Hit
CCAGACGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.15708228513387426No Hit
CCAGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.14999586625565436No Hit
CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC1270.14999586625565436No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA1270.14999586625565436No Hit
CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.14409051719047114No Hit
CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAA1170.1381851681252879No Hit
CCAGACGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT1100.12991767943403135No Hit
CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1090.1287366096209947No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAA1030.1216501907427748No Hit
CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAA960.11338270205151826No Hit
CCAGACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.10983949261240832No Hit
CCAGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.10983949261240832No Hit
CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.10393414354722508No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAC150.00802701250.58353434
CCTAACG150.00802701250.58353435
CTAACGG203.9298311E-450.58353436
GCCACAT150.00802701250.58353436
CCCTAAC150.00802701250.58353434
GCCCTAA150.00810362150.46222733
TCCCAGC150.00810362150.46222731
TGCGATC150.00810362150.46222732
TAGCCCT150.00810362150.46222731
GTGCGAT150.00810362150.46222731
TGATAAA150.00821955350.2813648
ACACGTG150.008238994550.25134343
CACGTGA150.008238994550.25134344
CGTGATA150.008238994550.25134346
ACGTGAT150.008238994550.25134345
TACACGT150.0082584750.2213642
CCAGTAC150.0082779850.19141438
TCGGAGA204.0838492E-450.19141450
ATAAAAG150.0082779850.19141450
GCGTATG252.0751013E-549.9827829
Produced by FastQC (version 0.11.5)