##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526449_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 84669 Sequences flagged as poor quality 0 Sequence length 56 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.477081340278026 39.0 38.0 40.0 36.0 40.0 2 38.16525528824009 39.0 38.0 40.0 35.0 40.0 3 37.719247894743056 39.0 38.0 40.0 33.0 40.0 4 37.79236792686816 39.0 38.0 40.0 34.0 40.0 5 37.492813190187675 39.0 38.0 40.0 33.0 40.0 6 38.085804721917114 39.0 38.0 40.0 35.0 40.0 7 38.137559201124375 39.0 38.0 40.0 36.0 40.0 8 38.33310893006885 39.0 38.0 40.0 36.0 40.0 9 38.15551146228253 39.0 38.0 40.0 35.0 40.0 10 37.054258347210904 39.0 37.0 40.0 33.0 40.0 11 37.40766986736586 39.0 38.0 40.0 33.0 40.0 12 37.30260189679812 39.0 37.0 40.0 33.0 40.0 13 37.224202482608746 39.0 37.0 40.0 33.0 40.0 14 37.210324912305566 39.0 37.0 40.0 33.0 40.0 15 37.12413043735015 39.0 36.0 40.0 33.0 40.0 16 37.01949946261323 39.0 36.0 40.0 33.0 40.0 17 37.06391949828154 39.0 36.0 40.0 33.0 40.0 18 37.03525493391914 39.0 36.0 40.0 33.0 40.0 19 36.75880192278166 39.0 36.0 40.0 32.0 40.0 20 37.0014763372663 39.0 36.0 40.0 33.0 40.0 21 36.731011350080905 38.0 36.0 40.0 32.0 40.0 22 36.41315003129835 38.0 36.0 40.0 31.0 40.0 23 36.25515832240844 38.0 35.0 40.0 30.0 40.0 24 36.137641876011294 38.0 35.0 40.0 30.0 40.0 25 36.164924588692436 38.0 35.0 40.0 30.0 40.0 26 35.833410102871184 38.0 35.0 40.0 30.0 40.0 27 35.848693146251875 38.0 35.0 40.0 30.0 40.0 28 35.59935749802171 38.0 35.0 40.0 29.0 40.0 29 35.72773978669879 38.0 35.0 40.0 29.0 40.0 30 35.54376454192207 38.0 35.0 40.0 29.0 40.0 31 35.352714688965264 38.0 35.0 40.0 28.0 40.0 32 35.106071879908825 38.0 35.0 40.0 27.0 40.0 33 35.264039967402475 38.0 35.0 40.0 27.0 40.0 34 35.360450696240655 39.0 35.0 40.0 28.0 40.0 35 35.11651253705607 39.0 35.0 40.0 27.0 40.0 36 35.10636714736208 39.0 35.0 40.0 27.0 40.0 37 34.73129480683603 39.0 35.0 40.0 25.0 40.0 38 34.826394548181746 39.0 35.0 40.0 26.0 40.0 39 34.84056738593818 39.0 35.0 40.0 26.0 40.0 40 34.43687772384226 39.0 35.0 40.0 23.0 40.0 41 34.40735097851634 39.0 35.0 39.0 24.0 40.0 42 34.3731471967308 39.0 35.0 40.0 24.0 40.0 43 34.245662521111626 39.0 35.0 40.0 23.0 40.0 44 33.71479526154791 39.0 34.0 39.0 18.0 40.0 45 33.66183609113135 39.0 33.0 39.0 19.0 40.0 46 33.158511379607646 39.0 33.0 39.0 7.0 40.0 47 33.036884810261135 39.0 33.0 39.0 4.0 40.0 48 32.66690288062927 38.0 32.0 39.0 4.0 40.0 49 32.6211718574685 38.0 32.0 39.0 4.0 40.0 50 32.28375202258206 38.0 32.0 39.0 4.0 40.0 51 31.691410079249785 38.0 32.0 39.0 4.0 40.0 52 31.46535331703457 38.0 32.0 39.0 4.0 40.0 53 31.036872999563005 37.0 31.0 39.0 4.0 40.0 54 30.563452975705395 37.0 31.0 39.0 4.0 40.0 55 29.859866066683203 36.0 29.0 39.0 4.0 40.0 56 17.332660123539902 21.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5014803577444056 1 2 0.27760593702817005 1 3 -0.76965280083013 1 4 -0.1628171353352954 1 5 0.08275638748721548 1 6 -0.4930877665847788 1 7 -1.1329028567103876 1 8 -1.5516327761562607 1 9 -1.1529227971139662 1 10 -0.42976273395849773 1 11 -0.2796402826080495 1 12 -0.8161249704463174 1 13 -0.5984859118524852 1 14 -0.8984698994293083 1 15 -0.9669276299723606 1 16 -1.8541810012894473 1 17 -1.2881605889116017 1 18 -1.4725643407678888 1 19 -1.8348310568870616 1 20 -2.059800349540474 1 21 -2.123219583023733 1 22 -1.736795264374372 1 23 -1.620127771952241 1 24 -1.6626968691606336 1 25 -2.3739341739384656 1 26 -0.4275909867230041 1 27 -0.8869987080970247 1 28 -1.503797794385342 1 29 -0.9919828838103939 1 30 -1.0974593004073725 1 31 -0.5946566212325806 1 32 -1.0831862145098583 1 33 -1.8651512677778115 1 34 -1.0363864204549813 1 35 -1.766894528445853 1 36 -1.5386567686486643 1 37 -1.0307727766133894 1 38 -1.435136912177306 1 39 -2.1489779409337046 1 40 -1.7695138876023648 1 41 -1.2270327169598545 1 42 -1.6479350004870383 1 43 -1.8408884287376708 1 44 -1.395405111882205 1 45 -1.4823016301750798 1 46 -0.9585772256705631 1 47 -0.05427430004257161 1 48 0.21434496769013123 1 49 -0.6572462375900372 1 50 -1.2786180037584423 1 51 -1.3465406714482135 1 52 -1.1300911500439632 1 53 -0.8253541427110669 1 54 -0.7468969788698843 1 55 -1.0975147399587968 1 56 0.285402070351914 2 1 0.198659698278 2 2 0.6706031359077187 2 3 0.31017913194298075 2 4 -0.026962793598599433 2 5 0.6201513454704113 2 6 0.28590383005387565 2 7 0.5916069472111829 2 8 0.14626638350760146 2 9 0.2735477911213309 2 10 1.0513297030162931 2 11 -0.32655905011505837 2 12 0.022250379693737443 2 13 -0.0322394132530448 2 14 0.5096533498704119 2 15 -0.013146117367320187 2 16 -0.3884947267796406 2 17 -0.21967319395361784 2 18 -0.5253654612160688 2 19 -0.4790887599682918 2 20 -0.4060188369354307 2 21 -0.08036244016658856 2 22 -0.22881207109706025 2 23 0.4590038807088206 2 24 0.08128072187578539 2 25 -0.10278571455470598 2 26 0.029972038487080965 2 27 -0.1017045904499625 2 28 0.1764543064549926 2 29 0.08784904896271684 2 30 0.3117843970716194 2 31 0.8070240510363291 2 32 1.0829202280671737 2 33 1.520282905891655 2 34 1.1454062966318475 2 35 1.081704911330057 2 36 2.025769001659455 2 37 2.190515738792776 2 38 2.3403532839011234 2 39 1.5123665968814208 2 40 1.7607382132379712 2 41 1.2022389917236183 2 42 1.1212526745829905 2 43 1.4002880418505654 2 44 1.7251831234119095 2 45 0.23212414013303828 2 46 -0.11375929849969424 2 47 0.19264446746443298 2 48 0.5191068724520349 2 49 0.3155828940626222 2 50 -0.15088691132146792 2 51 -0.08225495716249753 2 52 0.4434382617207433 2 53 0.5680632242357113 2 54 0.605764085555883 2 55 1.6709726549991863 2 56 -0.6837856047181141 3 1 -0.08597233635403256 3 2 0.19765941296399347 3 3 0.3199193916832428 3 4 -0.46094547758128357 3 5 0.04385264417170731 3 6 -0.2920182478682065 3 7 0.3832736138778472 3 8 0.15438326662448532 3 9 0.159911427484964 3 10 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1.9465156860811277 3 51 1.2716411467336073 3 52 1.1934382617207433 3 53 0.8207688519413381 3 54 1.8795736093654085 3 55 1.4972713562978868 3 56 -1.1664695874020978 4 1 0.1510406506589561 4 2 0.6586983740029595 4 3 0.6911315128953603 4 4 0.21113244449663426 4 5 1.0963418216608858 4 6 0.47638002053006545 4 7 0.2582736138778472 4 8 0.36055209779331676 4 9 0.38069064826418497 4 10 0.44418684587343193 4 11 0.42344094988494163 4 12 -0.15632104887768605 4 13 0.0272843962707654 4 14 -0.4427276025105371 4 15 -0.3345746887958896 4 16 -0.1861137743986916 4 17 -0.6363398606202821 4 18 -0.23965117550177695 4 19 -0.14575542663496321 4 20 -0.6798283607449491 4 21 -0.8065529163570702 4 22 -0.13357397585896535 4 23 -1.0052818335768947 4 24 -0.24014784955279112 4 25 -0.2813571431261366 4 26 -0.5533612948462547 4 27 -0.7445617333071084 4 28 -0.7640218840211972 4 29 -0.5669128557991883 4 30 0.14511773040495513 4 31 -0.4548807108684372 4 32 0.2614916566386043 4 33 -0.6106694750607247 4 34 0.41921582044137295 4 35 0.010276339901487574 4 36 0.5733880492785062 4 37 0.5595633578403962 4 38 0.4236866172344591 4 39 0.8814142159290412 4 40 0.7607382132379712 4 41 0.45223899172361826 4 42 0.6926812460115599 4 43 0.781240422802945 4 44 1.3323259805547636 4 45 0.16069556870446888 4 46 1.1719549872145905 4 47 0.7521682769882432 4 48 1.721487824832984 4 49 1.410820989300717 4 50 0.37292261248805403 4 51 0.32250694759940757 4 52 0.8839144521969331 4 53 1.4133013194738062 4 54 0.403383133174934 4 55 0.8852583692848981 4 56 0.13764296671045528 5 1 -0.04265682833263895 5 2 0.37172358408699324 5 3 0.06564131681692942 5 4 -0.06869948827647221 5 5 0.41216815219309666 5 6 -0.24602894305537149 5 7 0.30239126093667323 5 8 0.20130840031432484 5 9 0.058841908768393125 5 10 -0.5474097927820267 5 11 -1.291404988490406 5 12 -0.8043602645639609 5 13 -0.9396623824407158 5 14 -0.5219993111940155 5 15 -0.4963393946782446 5 16 -0.9600633542306198 5 17 -1.3352194124410133 5 18 -1.2019761054737685 5 19 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Count 4 147.0 5 22.0 6 23.0 7 30.0 8 99.0 9 113.0 10 82.0 11 138.0 12 145.0 13 137.0 14 211.0 15 253.0 16 235.0 17 292.0 18 356.0 19 418.0 20 382.0 21 491.0 22 534.0 23 523.0 24 636.0 25 748.0 26 762.0 27 952.0 28 1261.0 29 1560.0 30 1898.0 31 2560.0 32 3035.0 33 3721.0 34 5001.0 35 6158.0 36 7823.0 37 13568.0 38 16439.0 39 13916.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.17987260845416 0.5471455076163128 0.1867148816487633 0.08626700228075773 8 99.00908242686225 0.793678914360628 0.07204525859523557 0.12519340018188474 9 92.84507907262397 4.114847228619683 1.2224072564929311 1.8176664422634023 10 25.498116193648208 45.49126598873259 10.19145141669324 18.81916640092596 11 20.469120929738157 38.57019688433783 20.52699335057695 20.43368883534706 12 41.83231170794506 18.030211765817477 25.195762321510824 14.941714204726642 13 23.78438389493203 17.140866196600882 26.331951481652084 32.74279842681501 14 24.741050443491716 29.92358478309653 20.367548925817005 24.96781584759475 15 34.30653485927554 26.311873294830455 19.859688906211247 19.521902939682764 16 25.323317861318785 25.807556484663806 24.96427263815564 23.904853015861768 17 25.521737589908938 31.279452928462604 17.094804473892452 26.10400500773601 18 26.32132185333475 24.405626616589306 20.132516033022714 29.140535497053232 19 23.941466180065905 26.379194274173546 25.088284968524487 24.59105457723606 20 25.79220257709433 28.532284543339358 17.823524548536064 27.85198833103025 21 24.754042211435117 22.42497254012685 26.83154401256658 25.989441235871453 22 23.28361029420449 26.904770340974853 21.95254461491219 27.85907474990847 23 26.961461692000615 32.149901380670606 22.41316184199648 18.47547508533229 24 22.71132790815784 34.62151723812111 24.539667166664696 18.12748768705635 25 23.665276514301244 22.723674727975144 29.00535188970145 24.605696868022164 26 23.48910025403202 23.15236013469605 31.236486087316123 22.12205352395581 27 18.156239655743132 20.86820825649028 35.35726107722136 25.61829101054523 28 17.109182901456403 26.307452241346702 39.209381501796855 17.37398335540004 29 20.079950799507994 21.42705080896963 39.36512442047497 19.1278739710474 30 13.011161216253003 27.034288487258696 43.1618317177384 16.792718578749895 31 14.985085933431183 24.12054353487051 47.29297855215189 13.601391979546424 32 9.958668387830267 23.274790085150226 54.163360532455386 12.603180994564124 33 12.840135047088788 22.408339750044423 50.725581946336554 14.025943256530237 34 13.626292904280653 22.681658234896865 52.455481440233164 11.236567420589317 35 10.7122540886466 26.089120644702536 53.0149324484475 10.183692818203365 36 9.356773673479065 23.549006591113848 54.04476267248328 13.0494570629238 37 9.016207164643262 22.05729322408947 57.406853657077924 11.519645954189343 38 8.907261986666983 22.940143768831106 57.6890227989846 10.463571445517307 39 8.581731054360848 22.84626424852029 57.001198002538345 11.57080669458052 40 9.009426672848091 22.898612745622483 58.69857232861405 9.393388252915374 41 9.360904040643844 24.43734865391007 57.41892597692417 8.782821328521912 42 10.56368969161531 21.409996081365108 57.70605488463776 10.320259342381817 43 12.033835881716982 22.138266148673534 58.225517102089796 7.602380867519693 44 11.263236709808536 22.38147870835859 58.697899953649234 7.657384628183644 45 9.47892647443364 25.930063945610577 57.29431620985571 7.296693370100078 46 8.07324178110695 24.881992747161284 58.132096783782174 8.912668687949585 47 8.477074690029838 25.932882395596817 58.38851178659312 7.201531127780221 48 11.13002487887914 25.779994524265838 55.96914542835716 7.120835168497863 49 10.693418061951853 25.82794854402437 56.22791046374639 7.250722930277391 50 9.765095080562629 25.82883118737654 56.458100292738656 7.947973439322179 51 8.470543447225658 26.449033977348435 57.019130103740366 8.061292471685542 52 8.572176728242239 27.719720053323176 54.65863645019996 9.049466768234621 53 9.49699386868266 29.212453122209652 53.623429966069416 7.667123043038276 54 9.831985806313332 28.992272061537726 53.519248401424136 7.656493730724807 55 9.134214600452543 30.331070620459688 52.11146838156484 8.423246397522925 56 9.851976230455026 30.40143855763162 51.205744703654744 8.540840508258608 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 4.5 9 7.0 10 8.0 11 9.0 12 92.5 13 146.5 14 117.0 15 159.5 16 202.0 17 274.0 18 346.0 19 939.0 20 1532.0 21 2020.0 22 2650.5 23 2793.0 24 3259.5 25 3726.0 26 4494.0 27 5262.0 28 5840.0 29 6629.5 30 6841.0 31 7207.5 32 7574.0 33 7141.5 34 6709.0 35 5428.0 36 4147.0 37 4098.0 38 2935.0 39 1821.0 40 1621.5 41 1422.0 42 1475.5 43 1529.0 44 1542.0 45 1555.0 46 1647.0 47 1726.0 48 1713.0 49 1616.0 50 1519.0 51 1501.5 52 1484.0 53 1678.5 54 2300.5 55 2728.0 56 3062.0 57 3396.0 58 3114.0 59 2832.0 60 2463.0 61 2094.0 62 1969.0 63 1734.5 64 1625.0 65 1346.5 66 1068.0 67 904.0 68 740.0 69 614.0 70 488.0 71 390.5 72 225.0 73 157.0 74 145.5 75 134.0 76 115.5 77 97.0 78 78.5 79 39.0 80 18.0 81 16.0 82 14.0 83 9.0 84 4.0 85 2.5 86 1.0 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05669135102575913 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0023621396260732975 25 0.030707815138952863 26 0.040156373643246056 27 0.09566665485596854 28 0.09094237560382194 29 0.1381851681252879 30 0.21259256634659673 31 0.21849791541177999 32 0.2716460569984292 33 0.29999173251130873 34 0.3141645702677485 35 0.34132917596759144 36 0.369674851480471 37 1.256658281070994 38 0.43345262138445 39 0.4275472723192668 40 0.6448641179180101 41 0.5019546705405756 42 0.5385678347447118 43 0.588172766892251 44 0.6236048612833505 45 0.6318723499746071 46 0.6661233745526698 47 0.6472262575440835 48 0.7818682162302615 49 0.7511604010913085 50 0.7499793312782719 51 0.7239957953914655 52 0.7712385879129315 53 0.7960410539867011 54 0.8125760313692143 55 0.8255677993126175 56 0.8220245898735073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 84669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.27887420425421 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49024037592626 39.465447802619614 2 11.618922826676306 12.148484096894968 3 4.73070666907645 7.419480565496227 4 2.4331285017169706 5.088048754561882 5 1.475239472257365 3.856192939564658 6 0.9330381348273992 2.926690996704815 7 0.6483824326766673 2.3727692543906267 8 0.535423820712091 2.2393083655174855 9 0.384059280679559 1.8070368139460724 >10 1.6446773902042293 13.355537445818422 >50 0.054220133742996564 1.9003413291759677 >100 0.04518344478583047 3.895168243394867 >500 0.004518344478583047 1.5483825248910463 >1k 0.0022591722392915235 1.9771108670233497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCAGACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1674 1.9771108670233497 No Hit CCAGACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 775 0.9153291051034027 No Hit CCAGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 536 0.6330534197876436 No Hit CCAGACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 0.3862098288629841 No Hit CCAGACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 275 0.32479419858507835 No Hit CCAGACGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 267 0.31534564008078514 No Hit CCAGACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 0.2751892664375391 No Hit CCAGACGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.2598353588680627 No Hit CCAGACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 165 0.194876519151047 No Hit CCAGACGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 155 0.18306582102068053 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAAA 155 0.18306582102068053 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGCCAAAA 154 0.18188475120764389 No Hit CCAGACGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138 0.1629876341990575 No Hit CCAGACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.1582633549469109 No Hit CCAGACGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 133 0.15708228513387426 No Hit CCAGACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.14999586625565436 No Hit CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 127 0.14999586625565436 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAAA 127 0.14999586625565436 No Hit CCAGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.14409051719047114 No Hit CCAGACGGGATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAAA 117 0.1381851681252879 No Hit CCAGACGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 110 0.12991767943403135 No Hit CCAGACGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 109 0.1287366096209947 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGCAAAA 103 0.1216501907427748 No Hit CCAGACGGGCATTACCACCCCTGAGACTGCCAAGGCACACAGGGGATAGGGGAAAA 96 0.11338270205151826 No Hit CCAGACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 93 0.10983949261240832 No Hit CCAGACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.10983949261240832 No Hit CCAGACGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88 0.10393414354722508 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0011810698130366485 0.0 0.0 0.0 0.0 9 0.0035432094391099457 0.0 0.0 0.0 0.0 10 0.01653497738251308 0.0 0.0 0.0 0.0 11 0.022440326447696324 0.0 0.0 0.0 0.0 12 0.02598353588680627 0.0 0.0 0.0 0.0 13 0.028345675512879566 0.0 0.0 0.0 0.0 14 0.04251851326931935 0.0 0.0 0.0 0.0 15 0.047242792521465946 0.0 0.0 0.0 0.0 16 0.05078600196057589 0.0 0.0 0.0 0.0 17 0.053148141586649184 0.0 0.0 0.0 0.0 18 0.06613990953005232 0.0 0.0 0.0 0.0 19 0.06850204915612562 0.0 0.0 0.0 0.0 20 0.09094237560382194 0.0 0.0 0.0 0.0 21 0.10039093410811513 0.0 0.0 0.0 0.0 22 0.12046912092973816 0.0 0.0 0.0 0.0 23 0.1287366096209947 0.0 0.0 0.0 0.0 24 0.1381851681252879 0.0 0.0 0.0 0.0 25 0.14290944737743447 0.0 0.0 0.0 0.0 26 0.14409051719047114 0.0 0.0 0.0 0.0 27 0.15353907569476433 0.0 0.0 0.0 0.0 28 0.19723865877712032 0.0 0.0 0.0 0.0 29 0.20550614746837687 0.0 0.0 0.0 0.0 30 0.21967898522481663 0.0 0.0 0.0 0.0 31 0.23267075316821978 0.0 0.0 0.0 0.0 32 0.2503868003637695 0.0 0.0 0.0 0.0 33 0.2598353588680627 0.0 0.0 0.0 0.0 34 0.26810284755931924 0.0 0.0 0.0 0.0 35 0.3354238269024082 0.0 0.0 0.0 0.0 36 0.35195880428492127 0.0 0.0 0.0 0.0 37 0.36495057222832444 0.0 0.0 0.0 0.0 38 0.37321806091958093 0.0 0.0 0.0 0.0 39 0.3838476892369108 0.0 0.0 0.0 0.0 40 0.39802052699335055 0.0 0.0 0.0 0.0 41 0.413374434562827 0.0 0.0 0.0 0.0 42 0.42518513269319347 0.0 0.0 0.0 0.0 43 0.43817690063659664 0.0 0.0 0.0 0.0 44 0.4499875987669631 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCCAC 15 0.008027012 50.583534 34 CCTAACG 15 0.008027012 50.583534 35 CTAACGG 20 3.9298311E-4 50.583534 36 GCCACAT 15 0.008027012 50.583534 36 CCCTAAC 15 0.008027012 50.583534 34 GCCCTAA 15 0.008103621 50.462227 33 TCCCAGC 15 0.008103621 50.462227 31 TGCGATC 15 0.008103621 50.462227 32 TAGCCCT 15 0.008103621 50.462227 31 GTGCGAT 15 0.008103621 50.462227 31 TGATAAA 15 0.008219553 50.28136 48 ACACGTG 15 0.0082389945 50.251343 43 CACGTGA 15 0.0082389945 50.251343 44 CGTGATA 15 0.0082389945 50.251343 46 ACGTGAT 15 0.0082389945 50.251343 45 TACACGT 15 0.00825847 50.22136 42 CCAGTAC 15 0.00827798 50.191414 38 TCGGAGA 20 4.0838492E-4 50.191414 50 ATAAAAG 15 0.00827798 50.191414 50 GCGTATG 25 2.0751013E-5 49.98278 29 >>END_MODULE