FastQCFastQC Report
Fri 17 Jun 2016
SRR1526448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303611
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.6251420403081575No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT15990.5266607599856395No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12090.398206916086703No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC9480.3122416513235686No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7440.24505040989950955No Hit
GAGGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA7030.23154628784859574No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6620.2180421657976819No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.20717299439084882No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6120.20157372427217723No Hit
GAGGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.19498634766197534No Hit
GAGGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA5700.1877402333907533No Hit
GAGGAGGGGTATACTTAATAAAGTTACTGCTGTCTGTCAGATAAAAAAAAAAAAAAAAAAAAAA5480.18049411911953125No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5280.17390674250932936No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4570.15052155554311275No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4480.1475572360685219No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4290.14129922828883013No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4280.14096985945832002No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3580.11791404132261347No Hit
GAGGAGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3410.11231477120394188No Hit
GAGGAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT3370.11099729588190152No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC3260.10737423874629048No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3220.1060567634242501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATG303.6544589E-758.22229458
TCTAAAC150.004640786458.13562450
ATAGCAC303.7134487E-758.0875941
TATTATC150.004656002458.0875944
ATTATCC150.004656002458.0875945
CGCAATG150.004656002458.0875941
GTGACCT258.577177E-658.0875941
GAATACA201.9930731E-458.08758540
AGTAATC201.9995958E-458.0492137
ATAATCG150.00468042558.0108931
CCGTTAA303.7518112E-758.0013230
GCCGTTA303.7603786E-757.9821829
CCTAACA258.695504E-657.95350327
TCCTAAC258.712517E-657.93440226
ACGACGT150.004704942557.934426
TGCGTAT150.00471416157.90576622
GATCGTC150.004720314457.8866929
TGCTTAT150.004720314457.88669219
ATCGTCA150.004720314457.88669210
TATTAGG150.004720314457.88669210
Produced by FastQC (version 0.11.5)