FastQCFastQC Report
Fri 17 Jun 2016
SRR1526447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293676
Sequences flagged as poor quality0
Sequence length64
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.49408191340116314No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT13390.45594464648115607No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.3357441534207766No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8070.27479262861112247No Hit
GAGGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA5630.19170786853539276No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.18728122148217763No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.18421661967610564No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5410.18421661967610564No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5080.17297974638717498No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.1716177011400319No Hit
GAGGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA4680.15935929391574388No Hit
GAGGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.1532300903035999No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4160.14165270570288344No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4070.13858810389681145No Hit
GAGGAGGGGTATACTTAATAAAGTTACTGCTGTCTGTCAGATAAAAAAAAAAAAAAAAAAAAAA4020.13688554733788258No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3640.12394611749002302No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3160.1076015745243057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC201.9602908E-458.2820637
CAATTCA150.004594624558.28205537
TAGTACG150.004594624558.28205536
CCGTTGT258.442474E-658.24201238
GTTAGGT150.004635412758.1521258
ACTCTCA600.058.14214749
TACTAAG150.00464801758.11225547
GCCCATA150.00465432958.09234244
CTCCAAC150.00465432958.09234242
AACGTAT201.9955264E-458.07244528
AAAACGT150.0046796458.01283326
CGTTAGC150.004724180357.87421422
ACGCTTA202.0346939E-457.84459711
TACGAAT150.004733765557.84459311
CGAATCA150.004733765557.84459313
CTAGGAT150.004733765557.84459311
TAATATC351.6729246E-857.8347321
CGCACTA150.004736963757.8347321
CGTAAAC202.0364106E-457.83472421
GTTAGAT150.004740163757.82486316
Produced by FastQC (version 0.11.5)