FastQCFastQC Report
Fri 17 Jun 2016
SRR1526446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271727
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT18790.6915028686880582No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG16510.6075951230462928No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11760.43278731962594813No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7850.2888928961788854No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.24031472764944228No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.235898530510402No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC6030.2219139062367744No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5840.21492159409996062No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.20866531481965353No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5330.1961527562590394No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5310.19541672340253272No Hit
GAGGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT5210.19173655911999912No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC4970.18290416484191854No Hit
GAGGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4940.18180011555715847No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC4700.1729677212790779No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC4580.1685515241400376No Hit
GAGGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC4270.15714301486418353No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC4150.15272681772514327No Hit
GAGGAGGGGTATACTTAATAAAGTTACTGCTGTCTGTCAGATAAAAAAAAAAAAAA4130.15199078486863654No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3210.11813327346932767No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3150.11592517489980751No Hit
GAGGAGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.10893286276299374No Hit
GAGGAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC2890.10635674776522024No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCATA150.00801078850.64024736
CTTGTTA150.00802860750.61179734
TCGTTGT150.00809419450.50775533
GACGACA203.9859308E-450.47945432
TTAATTG150.00827509650.22616238
GACCATT252.0283029E-550.22616240
TAATTGC150.00827509650.22616239
CTATTAT750.050.20750448
TTCTAAT355.1075403E-850.1981847
CATTGTA204.09739E-450.1981847
CATTCTA355.129914E-850.17022345
TGCGAAA204.1086707E-450.17022344
TAGTGAT150.00831163450.17022344
CGAGTAA150.00831773650.1609143
ACTTCGT150.00844663649.96616730
ATTGACT150.00844663649.96616730
AACGTAT150.00845898949.94769728
ACCGTAT150.00845898949.94769728
CAGTAGG150.00845898949.94769728
CCTAACA301.0578296E-649.91080527
Produced by FastQC (version 0.11.5)