##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	271727
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.208300978555684	39.0	38.0	40.0	35.0	40.0
2	36.98035896322412	39.0	36.0	40.0	32.0	40.0
3	38.00713583854383	39.0	38.0	40.0	35.0	40.0
4	37.793535423421304	39.0	38.0	40.0	35.0	40.0
5	36.21985301423855	38.0	35.0	39.0	30.0	40.0
6	38.09892649607878	39.0	38.0	40.0	35.0	40.0
7	38.134016862512745	39.0	38.0	40.0	36.0	40.0
8	37.57313038454036	39.0	37.0	40.0	32.0	40.0
9	37.47799813783687	39.0	36.0	40.0	32.0	40.0
10	36.38348783889713	39.0	35.0	40.0	31.0	40.0
11	37.02112414298174	39.0	35.0	40.0	32.0	40.0
12	37.24903671699904	39.0	36.0	40.0	32.0	40.0
13	37.30170722821067	39.0	37.0	40.0	33.0	40.0
14	37.279916975493784	39.0	37.0	40.0	33.0	40.0
15	37.38118773622055	39.0	37.0	40.0	33.0	40.0
16	37.150776330655404	39.0	36.0	40.0	33.0	40.0
17	37.1088482189845	39.0	36.0	40.0	33.0	40.0
18	37.02398731079355	39.0	36.0	40.0	33.0	40.0
19	36.891284266929674	39.0	36.0	40.0	32.0	40.0
20	36.78115903093914	39.0	36.0	40.0	32.0	40.0
21	36.63766942556316	38.0	36.0	40.0	32.0	40.0
22	36.58820801760591	38.0	36.0	40.0	31.0	40.0
23	36.361712306837376	38.0	35.0	40.0	31.0	40.0
24	36.153919190952685	38.0	35.0	40.0	30.0	40.0
25	36.134454802062365	38.0	35.0	40.0	30.0	40.0
26	35.63883235747644	38.0	35.0	39.0	29.0	40.0
27	35.499806791375164	38.0	35.0	39.0	29.0	40.0
28	35.280321793564866	38.0	35.0	39.0	29.0	40.0
29	35.12766121879681	38.0	34.0	39.0	28.0	40.0
30	34.90665263297353	38.0	34.0	39.0	27.0	40.0
31	34.26408122858604	38.0	33.0	39.0	25.0	40.0
32	34.13991248569336	38.0	33.0	39.0	25.0	40.0
33	34.08445240995558	38.0	33.0	39.0	24.0	40.0
34	34.0329117091787	38.0	33.0	39.0	24.0	40.0
35	33.80690546025975	37.0	33.0	39.0	23.0	40.0
36	33.246883820893764	38.0	33.0	39.0	18.0	40.0
37	32.78737850857662	37.0	32.0	39.0	12.0	40.0
38	32.80263278952773	37.0	32.0	39.0	16.0	40.0
39	32.69338343263643	37.0	32.0	39.0	14.0	40.0
40	32.25459008490139	36.0	32.0	39.0	5.0	40.0
41	31.701303146172446	36.0	31.0	39.0	4.0	40.0
42	31.51462681294093	36.0	30.0	39.0	4.0	40.0
43	31.375012420554455	36.0	30.0	39.0	4.0	40.0
44	30.912242802518705	36.0	30.0	39.0	4.0	40.0
45	30.72771936539247	35.0	30.0	39.0	4.0	40.0
46	30.070765878988837	35.0	29.0	39.0	4.0	40.0
47	29.82727516956357	35.0	28.0	39.0	4.0	40.0
48	29.41843835908835	35.0	27.0	39.0	4.0	40.0
49	29.301795552153447	35.0	27.0	39.0	4.0	40.0
50	28.89658738366081	35.0	26.0	39.0	4.0	40.0
51	28.118549132033255	35.0	23.0	39.0	4.0	40.0
52	27.876714496535126	34.0	22.0	39.0	4.0	40.0
53	27.462839541157116	34.0	17.0	38.0	4.0	40.0
54	27.12127613376661	34.0	5.0	38.0	4.0	40.0
55	26.578562307021386	33.0	4.0	38.0	4.0	40.0
56	15.608463641816972	19.0	4.0	23.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.8516896084863248
1	2	-1.1377031758566787
1	3	-1.1890812133705637
1	4	-0.7945590798293836
1	5	-0.17806877939694488
1	6	-0.9282649512040635
1	7	-1.2006408607762324
1	8	-1.58554920613458
1	9	-1.3622726324511376
1	10	-0.5049174385743171
1	11	-0.47438819967327817
1	12	-0.6485397125452437
1	13	-0.6617897274911684
1	14	-0.8769545613209857
1	15	-0.8017478224877195
1	16	-0.7679248903102334
1	17	-0.6650740358458549
1	18	-0.5942027990313363
1	19	-0.4444563734176583
1	20	-0.7663426841143277
1	21	-0.10219937170853655
1	22	-0.482434296998818
1	23	-0.26868860888644974
1	24	-0.4572889805654299
1	25	-0.2611503495006886
1	26	0.017019967149799697
1	27	-0.16070850688746674
1	28	-0.2243811891815426
1	29	-0.20631284140955586
1	30	-0.05755397625311787
1	31	0.7105522059256231
1	32	0.6136578724570114
1	33	0.599183827507126
1	34	1.0179427406280084
1	35	1.1804275477635926
1	36	1.0186274948218426
1	37	1.1874754562516259
1	38	1.7099970831102738
1	39	0.9065970356811732
1	40	1.8191497096662346
1	41	2.524834976233972
1	42	1.954272207232755
1	43	2.168781682837821
1	44	2.652220700584401
1	45	2.769737280853512
1	46	2.027682885064184
1	47	2.398517859576895
1	48	2.695444951099301
1	49	2.3238745175093847
1	50	2.6522765159203274
1	51	2.996908829763104
1	52	3.1608287384326417
1	53	3.322912091795569
1	54	2.699914198811477
1	55	2.9906213747706545
1	56	2.4825653499981417
2	1	0.08816521349777418
2	2	0.49782772946336706
2	3	0.06560879071452774
2	4	0.07083701733304082
2	5	0.3029355256654256
2	6	-0.10101736298170749
2	7	0.3506585138898899
2	8	0.10659169824275949
2	9	0.35733268445801514
2	10	0.38871295453758137
2	11	-0.25146767049626817
2	12	-0.16605848827348524
2	13	-0.1196189704915156
2	14	-0.053619237812831955
2	15	-0.09046981601441217
2	16	-0.3456516916803096
2	17	-0.044076644022325695
2	18	-0.2998716548903033
2	19	0.15471718030134696
2	20	-0.3109016508993605
2	21	-0.05863025503192176
2	22	-0.04168389634519798
2	23	-0.3966306633753405
2	24	-0.019743808834292054
2	25	0.27164039932877415
2	26	0.031349225862051355
2	27	-0.11387131366244319
2	28	-0.10071277325594963
2	29	-0.023489097576479878
2	30	0.586649901445405
2	31	0.30551496870725714
2	32	0.4072976927128025
2	33	0.5002616642918412
2	34	0.35452320130110593
2	35	0.4227679933532613
2	36	0.30860644086715183
2	37	0.5473409623321288
2	38	0.5468760700065403
2	39	0.6830008945254065
2	40	0.18250325898591058
2	41	0.7146348065453871
2	42	0.6289886073207427
2	43	0.6031373375529654
2	44	1.1232793688432068
2	45	1.1123354017094975
2	46	0.5609921268075766
2	47	0.952676801222875
2	48	1.2395011368768856
2	49	0.7951060167395028
2	50	0.4066837056887316
2	51	1.0835285702024073
2	52	1.3474547636345413
2	53	1.8753427237284477
2	54	1.6949021006434855
2	55	1.025580366694733
2	56	0.15523920224337218
3	1	-0.0064807632774801505
3	2	0.8833408906419322
3	3	0.07525396005551954
3	4	0.37811713830137705
3	5	0.38519105659015906
3	6	0.26342910412635945
3	7	0.3716811660912498
3	8	0.29504685627966865
3	9	0.18918082809322811
3	10	0.7317844344911961
3	11	0.2916860209464005
3	12	0.15071999580537465
3	13	0.2530926873912449
3	14	0.04180481198383745
3	15	0.05210839500486486
3	16	-0.013561317764839487
3	17	0.31166421361967167
3	18	0.035316330487795256
3	19	0.11610714169130887
3	20	0.08938021706311616
3	21	-0.16916066594256307
3	22	-0.17234144145141528
3	23	0.2512545928749361
3	24	-0.21050382468936846
3	25	-0.3633552698874283
3	26	0.33933401673657926
3	27	0.293310447131482
3	28	0.08761025917354459
3	29	0.2789610984098374
3	30	0.43118210339205376
3	31	0.41014376915354944
3	32	0.5504778092769484
3	33	0.1260672634814668
3	34	0.3670604533820807
3	35	0.47156638435697573
3	36	0.6734346041744033
3	37	1.59655041078112
3	38	0.6181945367622674
3	39	1.1500795973455524
3	40	1.0981630795886943
3	41	0.9893679862215272
3	42	0.8920653722986458
3	43	1.0641676236783049
3	44	1.0368692215965574
3	45	1.4825073118053638
3	46	1.310349849549329
3	47	0.8972574492255774
3	48	1.807685290612369
3	49	1.5124893424878465
3	50	1.710367074010886
3	51	1.9761838544926746
3	52	2.0013150334643903
3	53	2.1989770587696285
3	54	2.064428063325341
3	55	1.9754166777477735
3	56	0.547931188091253
4	1	-0.06525382205053631
4	2	0.10331086061189865
4	3	-0.297011162209607
4	4	-0.4009476407633983
4	5	-0.24533232393321924
4	6	-0.28622769553044236
4	7	0.08071162512170815
4	8	-0.29429248305967093
4	9	-0.19531080639840326
4	10	-0.31326061055385424
4	11	-0.6071771279167493
4	12	-0.7885764434910669
4	13	-0.3360321517335976
4	14	-0.8394693192902878
4	15	-0.7866946937982249
4	16	-0.9992970535005767
4	17	-1.3203606684052147
4	18	-0.8405952954238316
4	19	-0.8611558355716724
4	20	-0.8601049824220794
4	21	-0.7906750374569285
4	22	-0.9228776919876651
4	23	-1.0376771960568547
4	24	-1.1521597663453065
4	25	-0.8941360506682088
4	26	-0.7360628086602432
4	27	-0.22889032506928686
4	28	-0.4573275357642501
4	29	-0.6984091289603924
4	30	-0.3230006507906964
4	31	-0.13189827288849187
4	32	0.35860736740650623
4	33	0.4277618151760194
4	34	0.14591073223235895
4	35	-0.27661036381977055
4	36	0.3328082635480669
4	37	0.23404504827576034
4	38	0.1313862999540305
4	39	0.455527902793861
4	40	0.8459108273364393
4	41	0.981216978070524
4	42	1.118577598810873
4	43	0.7950771045877865
4	44	1.5834157681431051
4	45	0.6992597785578312
4	46	0.8734129126123911
4	47	0.43790523987336627
4	48	1.040847023774102
4	49	0.8921547221532258
4	50	0.5288998925437021
4	51	1.0460036743124945
4	52	1.383018165167524
4	53	0.9597020694946394
4	54	0.40698490588218306
4	55	1.1109808133119117
4	56	0.34351248367254783
5	1	0.12397252465612496
5	2	0.34687416519882674
5	3	0.07691084753917465
5	4	0.09374052321609838
5	5	0.3450001345094762
5	6	-0.25601272240208317
5	7	0.37526855550540716
5	8	0.17774226259712123
5	9	0.1546142029754236
5	10	-0.05548033308853206
5	11	0.24074813142583196
5	12	0.302849628249966
5	13	0.0032066934107604084
5	14	0.027516057537603444
5	15	-0.03500740470936137
5	16	0.0038588020174827875
5	17	0.09493117011497532
5	18	0.11087952022421632
5	19	-0.4082749456829049
5	20	-0.37482189280828493
5	21	-0.2384003217963908
5	22	0.04437640125068043
5	23	0.09374387630909808
5	24	-0.223150892454548
5	25	-0.293492381126903
5	26	-0.08192582578310237
5	27	-0.3410950494787386
5	28	-0.01510156401072038
5	29	-0.016588106588187657
5	30	0.019331557683244682
5	31	0.40678439177842307
5	32	0.0044953814047588025
5	33	-0.39072962339415795
5	34	-0.32340560322570866
5	35	-0.16212467453093637
5	36	-0.5915761883954431
5	37	-3.4342464152912946
5	38	-0.44894740833242963
5	39	-1.1194002311893954
5	40	-0.46080083370592817
5	41	-0.7357559003196776
5	42	-0.5036571218490451
5	43	-0.7840067599291451
5	44	-0.4233958804008289
5	45	-0.5196816037693779
5	46	0.27236304384598853
5	47	0.03123732336293017
5	48	0.11690001714993059
5	49	-0.4074328294028291
5	50	-0.5127199049816049
5	51	-0.15881447341903865
5	52	-0.15732054249401983
5	53	0.03616126209556114
5	54	-0.3559197310382025
5	55	-0.2164782543046364
5	56	-0.3050314483359511
6	1	-0.01744353327639203
6	2	-0.12680005925805915
6	3	-0.2587151209015133
6	4	-0.4550688876557345
6	5	-0.29118239086762543
6	6	-0.16980964236540075
6	7	0.21835744650246625
6	8	0.10793547639720202
6	9	0.10259510295328766
6	10	-0.002876215832110063
6	11	-0.41866115928029757
6	12	-0.10517617575344929
6	13	-0.4615341597657263
6	14	-0.1680694340349831
6	15	-0.10554150963299236
6	16	-0.44790099306837305
6	17	-0.2349236823658174
6	18	-0.6137354751905164
6	19	-0.6966884421886164
6	20	-0.5042530030761228
6	21	-0.12568173089736234
6	22	-0.3366279310391107
6	23	-0.7407753027694213
6	24	-0.6539765573187282
6	25	-0.3734341956290024
6	26	-0.0863687945084024
6	27	-0.42266542547089614
6	28	0.05476846729560947
6	29	-0.22360515114436907
6	30	-0.20022382921869308
6	31	-0.030444840109758786
6	32	-0.02927750541812202
6	33	-0.22948622460214096
6	34	-0.2504365617052926
6	35	-0.43170694060309955
6	36	-0.3491576955263298
6	37	-0.94017564401722
6	38	-0.17742035523816213
6	39	-0.7209876833602813
6	40	-0.1707271531569603
6	41	-1.0201217100151538
6	42	-0.5658744952797754
6	43	-0.6381319219947308
6	44	-0.38808929974750583
6	45	-0.40147649988929146
6	46	-0.3494786536526675
6	47	0.14248546553793062
6	48	0.2618261784881959
6	49	0.1055798512410071
6	50	-0.009826441363355087
6	51	-0.32333080646776935
6	52	-0.03317039861140003
6	53	-0.2536752493043686
6	54	-0.11443410348863381
6	55	-0.6208990872426874
6	56	-0.28060302598513154
7	1	0.04664488145122192
7	2	0.11218642101417942
7	3	-0.02265638212963239
7	4	-0.06791722758031682
7	5	0.08948531813633309
7	6	-0.1206818334196953
7	7	0.3711211731648447
7	8	0.020532429093485405
7	9	0.08867683445946284
7	10	0.13094149777039377
7	11	-0.36341728301186293
7	12	-0.23090044688377276
7	13	-0.36720263519415397
7	14	-0.4155325690176639
7	15	-0.7155387505048765
7	16	-0.4649156164473851
7	17	-0.7004345809218719
7	18	-0.701766990564991
7	19	-0.5933744227520847
7	20	-0.820755654904815
7	21	-1.1174350035298772
7	22	-0.6617605224735499
7	23	-0.5189567307715066
7	24	-0.32833812572977905
7	25	-0.6238404006015656
7	26	-0.5995972809733416
7	27	-0.45217276781738036
7	28	-0.16867492459255828
7	29	-0.1529408144081046
7	30	-0.2170391822338118
7	31	-0.7573677991213756
7	32	-0.3464392822966289
7	33	-0.16187225728247512
7	34	-0.15192035949186078
7	35	-0.7025766790990673
7	36	-0.6060018661017565
7	37	-0.9481311300836239
7	38	-0.7160328203658501
7	39	-1.0717954947585469
7	40	-0.6446380639758083
7	41	-0.6583080431338892
7	42	-0.29836169599101936
7	43	-0.23330651349048637
7	44	-0.13662542899733054
7	45	-1.1177461586771145
7	46	-0.5782410314079627
7	47	-0.7051033630588996
7	48	-0.47981213047284044
7	49	-0.7470673789009368
7	50	-0.5697914924072229
7	51	-0.026969789882343775
7	52	-0.2323641200620905
7	53	0.08986685805637151
7	54	-0.256713143312048
7	55	-0.384717720554562
7	56	0.0547689964557776
8	1	-0.049071992639134976
8	2	0.39577272213990966
8	3	0.18719302530197268
8	4	0.20655035194860005
8	5	0.6432323107403661
8	6	0.10554859994235244
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117	1	-0.06812317491989006
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117	3	-0.11660823180667279
117	4	-0.17939696921273196
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118	42	0.7139988442321155
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118	51	1.3764066047154238
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119	1	0.02756091198557442
119	2	-0.013767777459101183
119	3	-0.2096792828929921
119	4	0.10765529150365438
119	5	0.05895096699128999
119	6	-0.1626665662441198
119	7	0.3329532342335426
119	8	0.0625171619179099
119	9	0.07340965888694484
119	10	0.11567432219787577
119	11	0.01444531240797886
119	12	0.27673314090249335
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119	14	-0.4155325690176639
119	15	0.14323987544932493
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119	18	-0.04146164705353783
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119	20	-0.5955648152101602
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119	35	-0.2903629386410529
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119	40	1.141621478008922
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119	45	1.4280553680404395
119	46	1.4370261441645553
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119	51	1.5799004391252893
119	52	1.6378648875714994
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119	54	1.7623708261535995
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120	1	-0.24562900242571573
120	2	-0.22930097199993327
120	3	-0.3430068332052727
120	4	-0.6134296032453648
120	5	0.014768686167791145
120	6	-0.48680489610764255
120	7	-0.20752791311782914
120	8	-0.5314858452530373
120	9	-0.5730164341040336
120	10	-0.503195675488918
120	11	-0.09869949443911707
120	12	-0.2873138172284371
120	13	-0.32466851536995733
120	14	-0.31944767426864473
120	15	-0.4928635249670563
120	16	-0.4166852208887448
120	17	-0.6578426308871741
120	18	-0.4578391626676961
120	19	-0.6596767590925907
120	20	-0.8238495711666687
120	21	-0.42830130008319145
120	22	-0.4641836082935811
120	23	-0.6426555510352117
120	24	-0.2210630852486304
120	25	-0.2601172916494505
120	26	-0.4977871953846318
120	27	0.08929149311253326
120	28	-0.44235639579311226
120	29	-0.17910898466024605
120	30	0.27440194661190276
120	31	0.2656486246584038
120	32	0.18562756942670688
120	33	0.3732885107027144
120	34	0.4745543108759378
120	35	0.5812539940445873
120	36	0.398104078843879
120	37	0.8004231146538245
120	38	0.7964295899973237
120	39	0.36389731116326374
120	40	0.7306510870767013
120	41	0.6983886952422402
120	42	1.0568892871225586
120	43	0.7097596442703278
120	44	0.5706091303364644
120	45	1.1285527078507585
120	46	0.3014432156426956
120	47	0.6471404491085764
120	48	0.39275900068607683
120	49	0.5604447654432683
120	50	0.932579546223355
120	51	0.6060686093774272
120	52	0.41530532245468166
120	53	0.19653468132725038
120	54	0.2491566230538993
120	55	0.37595195328304953
120	56	0.36858463374469785
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	325.0
5	52.0
6	131.0
7	103.0
8	210.0
9	244.0
10	283.0
11	388.0
12	509.0
13	553.0
14	854.0
15	994.0
16	1255.0
17	1451.0
18	1841.0
19	2109.0
20	2163.0
21	2774.0
22	2934.0
23	3355.0
24	4703.0
25	4962.0
26	4254.0
27	4781.0
28	5586.0
29	6498.0
30	7701.0
31	9354.0
32	10982.0
33	13502.0
34	17456.0
35	22534.0
36	30145.0
37	40653.0
38	41180.0
39	24908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.98265393664691	0.7257289507306997	0.19220218786475152	0.0994149247576301
8	98.57320030766174	0.9704593213041031	0.29294107688967236	0.16339929414449061
9	96.26316118751541	2.0277705196759985	0.8276689471418004	0.8813993456667906
10	47.25882963415487	32.705251962447605	8.588399386148597	11.447519017248931
11	32.79909615165221	30.662760785641467	18.57158103537742	17.9665620273289
12	38.93393001063568	16.509216971445607	23.2339811649192	21.322871852999516
13	25.48808178797102	17.344246247152473	27.025654425213542	30.14201753966297
14	24.2912923632911	23.344386093395208	29.67905287292025	22.685268670393448
15	30.320873523794102	20.941238816900785	26.463693339270666	22.274194320034447
16	29.031343959194338	22.667971898265538	25.41411048589209	22.886573656648032
17	31.37413654145521	21.071148616074222	23.067637739348683	24.487077103121884
18	30.00401137906796	22.55535887122001	23.694369716664152	23.746260033047875
19	28.722946192318027	23.130936565008263	24.494805448115205	23.65131179455851
20	27.15887637224126	26.32421511296264	23.42792582260872	23.08898269218738
21	26.394874267187284	21.429228600764738	28.673631990932076	23.5022651411159
22	27.328899962094304	20.619592458607354	26.586242809879035	25.465264769419306
23	27.331576661784297	24.16883183795441	25.762716854478406	22.736874645782883
24	26.805580601118034	25.070383141903456	26.097516993158575	22.02651926381994
25	28.84845494810555	22.35492932855686	25.433987828091116	23.362627895246476
26	26.003063689389027	20.39157780003535	28.59504801743946	25.010310493136156
27	24.18554953759994	17.651155079031724	30.75089348218562	27.412401901182715
28	24.378930614861456	21.80144200834847	30.04085813128392	23.778769245506158
29	26.76153934046168	20.862773717246995	30.720464706554473	21.655222235736847
30	23.586694306939822	20.891029944448952	33.805357393158296	21.71691835545293
31	23.9843591493443	20.6724828005976	35.901654462622425	19.441503587435673
32	24.863326455051844	19.44990974562663	36.443571636871035	19.24319216245049
33	24.872714115349257	19.614025630706635	35.96938492949894	19.543875324445168
34	20.85826131915208	21.13265381490509	40.392569613708545	17.616515252234286
35	19.778161912993376	24.10226861393672	40.170570810155944	15.94899866291396
36	22.185398655139288	22.65609990393852	37.38601936008276	17.77248208083943
37	21.967208224418947	22.473217315429736	38.25011093419645	17.309463525954875
38	22.575361509707427	22.143879199742663	38.4625623467905	16.818196943759407
39	21.60549288431888	20.26702753467254	38.12370821455377	20.003771366454803
40	21.838620515898597	20.5270139089582	38.88431926135058	18.750046313792616
41	19.473129220238647	21.25834797891037	39.10313569512533	20.16538710572565
42	18.980965739812063	20.236346073420506	40.06336117721514	20.71932700955229
43	19.278183683218476	19.332619861428448	41.47777559703897	19.91142085831411
44	20.535466208281314	19.50150592924911	38.88890947012777	21.074118392341802
45	20.08247132398708	20.60301135184801	38.55923412075047	20.755283203414447
46	19.459403364552486	21.370727133236723	37.96370078535875	21.20616871685204
47	19.173620477552653	22.04456861669804	39.00902636766515	19.772784538084156
48	19.321133324428217	23.303946450569185	38.486798165546105	18.888122059456492
49	21.55337428774929	22.373204534662868	37.145358499525166	18.92806267806268
50	18.985740570377185	22.401845861649406	37.676573582218005	20.935839985755408
51	18.47832537162864	24.58423582470403	38.17278877251283	18.7646500311545
52	19.43673469387755	23.823376623376625	37.528385899814474	19.211502782931355
53	19.774269214210424	23.0655888596921	38.38462566249499	18.775516263602487
54	19.712454015286784	23.483664522260128	36.589539800210105	20.214341662242976
55	20.826000757390123	23.594558671745634	35.40835950784493	20.171081063019315
56	19.249488608309235	23.135322965663434	35.1481086860482	22.467079739979138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	6.0
9	10.0
10	55.5
11	101.0
12	383.0
13	491.5
14	318.0
15	365.5
16	413.0
17	725.0
18	1037.0
19	1971.0
20	2905.0
21	3539.5
22	4287.0
23	4400.0
24	4866.0
25	5332.0
26	6204.5
27	7077.0
28	7996.5
29	9800.0
30	10684.0
31	10817.5
32	10951.0
33	10647.5
34	10344.0
35	9371.5
36	8399.0
37	8048.0
38	6311.5
39	4926.0
40	4470.5
41	4015.0
42	4131.0
43	4247.0
44	4297.0
45	4347.0
46	4947.0
47	6908.5
48	8270.0
49	7423.5
50	6577.0
51	6678.5
52	6780.0
53	7500.0
54	9102.5
55	9985.0
56	10681.0
57	11377.0
58	11923.0
59	12469.0
60	13347.0
61	14225.0
62	16117.0
63	17815.0
64	17621.0
65	15721.5
66	13822.0
67	12088.5
68	10355.0
69	9622.5
70	8890.0
71	7459.0
72	5079.5
73	4131.0
74	3611.5
75	3092.0
76	2707.5
77	2323.0
78	1846.0
79	1037.0
80	705.0
81	577.0
82	449.0
83	368.5
84	288.0
85	199.0
86	110.0
87	92.0
88	50.5
89	27.0
90	21.5
91	16.0
92	9.5
93	3.0
94	4.0
95	5.0
96	2.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0507862670989633
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	3.6801642825335724E-4
24	0.0
25	0.043425938533896154
26	0.0585146120922838
27	0.11666120775631424
28	0.11040492847600716
29	0.15309483415339661
30	0.23001026765834828
31	0.23626654693865534
32	0.3039815697372731
33	0.32385445686295433
34	0.3485115575559293
35	0.3639682475425703
36	0.39267352894633223
37	1.3064583202994182
38	0.4651727653122435
39	0.46664483102525695
40	0.6731020472753904
41	0.5343598538238746
42	0.5630651352276366
43	0.6204756980351602
44	0.660221472286523
45	0.6679498172798434
46	0.7043834436769257
47	0.6811984086969642
48	0.8166284542941996
49	0.7945474685989983
50	0.7916033371729714
51	0.7739385486168103
52	0.8195725857202265
53	0.8431256371284415
54	0.8626305078258694
55	0.8762471156712436
56	0.8699908363909366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	271727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.19626060988332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.1614508579871	26.71673991377418
2	12.706917608992379	9.961273083007615
3	5.968360100812226	7.018121937751964
4	3.503479559852565	5.492931914775219
5	2.2110565828305955	4.333257502191304
6	1.5734288445088118	3.700351622428498
7	1.1063542278226965	3.035546404840924
8	0.8095971483699959	2.5386544653222987
9	0.6223836041842121	2.195559895403056
>10	3.0730808545796826	20.648018135916395
>50	0.17227575675595735	4.614773492294181
>100	0.07966508982934445	5.793154713070306
>500	0.008962322605801251	2.114762985811543
>1k	0.002987440868600417	1.8368539334125362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGGAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1879	0.6915028686880582	No Hit
GAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1651	0.6075951230462928	No Hit
GAGGAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1176	0.43278731962594813	No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	785	0.2888928961788854	No Hit
GAGGAGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	653	0.24031472764944228	No Hit
GAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	641	0.235898530510402	No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	603	0.2219139062367744	No Hit
GAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	584	0.21492159409996062	No Hit
GAGGAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.20866531481965353	No Hit
GAGGAGGGGGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	533	0.1961527562590394	No Hit
GAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	531	0.19541672340253272	No Hit
GAGGAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	521	0.19173655911999912	No Hit
GAGGAGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	497	0.18290416484191854	No Hit
GAGGAGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	494	0.18180011555715847	No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	470	0.1729677212790779	No Hit
GAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	458	0.1685515241400376	No Hit
GAGGAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	427	0.15714301486418353	No Hit
GAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	415	0.15272681772514327	No Hit
GAGGAGGGGTATACTTAATAAAGTTACTGCTGTCTGTCAGATAAAAAAAAAAAAAA	413	0.15199078486863654	No Hit
GAGGAGGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	321	0.11813327346932767	No Hit
GAGGAGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	315	0.11592517489980751	No Hit
GAGGAGGGGAGTGCAGTGCTGAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	296	0.10893286276299374	No Hit
GAGGAGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC	289	0.10635674776522024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.007728344993320502	0.0	0.0	0.0	0.0
6	0.007728344993320502	0.0	0.0	0.0	0.0
7	0.007728344993320502	0.0	0.0	0.0	0.0
8	0.007728344993320502	0.0	0.0	0.0	0.0
9	0.008096361421573859	0.0	0.0	0.0	0.0
10	0.01987288712568129	0.0	0.0	0.0	0.0
11	0.026865199262495077	0.0	0.0	0.0	0.0
12	0.02760123211900179	0.0	0.0	0.0	0.0
13	0.028705281403761863	0.0	0.0	0.0	0.0
14	0.034961560684068936	0.0	0.0	0.0	0.0
15	0.03716965925358908	0.0	0.0	0.0	0.0
16	0.040113790679615936	0.0	0.0	0.0	0.0
17	0.04232188924913608	0.0	0.0	0.0	0.0
18	0.0456340371034163	0.0	0.0	0.0	0.0
19	0.048578168529443154	0.0	0.0	0.0	0.0
20	0.056306513522763654	0.0	0.0	0.0	0.0
21	0.05777857923577708	0.0	0.0	0.0	0.0
22	0.06219477637481737	0.0	0.0	0.0	0.0
23	0.0713951870811513	0.0	0.0	0.0	0.0
24	0.07691543350495166	0.0	0.0	0.0	0.0
25	0.07985956493097852	0.0	0.0	0.0	0.0
26	0.08390774564176545	0.0	0.0	0.0	0.0
27	0.08537981135477887	0.0	0.0	0.0	0.0
28	0.09715633705888631	0.0	0.0	0.0	0.0
29	0.10230856705443331	0.0	0.0	0.0	0.0
30	0.10893286276299374	0.0	0.0	0.0	0.0
31	0.11298104347378067	0.0	0.0	0.0	0.0
32	0.11629319132806089	0.0	0.0	0.0	0.0
33	0.11886930632583438	0.0	0.0	0.0	0.0
34	0.12438955274963474	0.0	0.0	0.0	0.0
35	0.1427903741623026	0.0	0.0	0.0	0.0
36	0.14683855487308953	0.0	0.0	0.0	0.0
37	0.1497826862991164	0.0	0.0	0.0	0.0
38	0.15309483415339661	0.0	0.0	0.0	0.0
39	0.15640698200767683	0.0	0.0	0.0	0.0
40	0.16045516271846375	0.0	0.0	0.0	0.0
41	0.1645033434292507	0.0	0.0	0.0	0.0
42	0.17149565556606447	0.0	0.0	0.0	0.0
43	0.17664788556161148	0.0	0.0	0.0	0.0
44	0.18106408270065175	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCATA	15	0.008010788	50.640247	36
CTTGTTA	15	0.008028607	50.611797	34
TCGTTGT	15	0.008094194	50.507755	33
GACGACA	20	3.9859308E-4	50.479454	32
TTAATTG	15	0.008275096	50.226162	38
GACCATT	25	2.0283029E-5	50.226162	40
TAATTGC	15	0.008275096	50.226162	39
CTATTAT	75	0.0	50.207504	48
TTCTAAT	35	5.1075403E-8	50.19818	47
CATTGTA	20	4.09739E-4	50.19818	47
CATTCTA	35	5.129914E-8	50.170223	45
TGCGAAA	20	4.1086707E-4	50.170223	44
TAGTGAT	15	0.008311634	50.170223	44
CGAGTAA	15	0.008317736	50.16091	43
ACTTCGT	15	0.008446636	49.966167	30
ATTGACT	15	0.008446636	49.966167	30
AACGTAT	15	0.008458989	49.947697	28
ACCGTAT	15	0.008458989	49.947697	28
CAGTAGG	15	0.008458989	49.947697	28
CCTAACA	30	1.0578296E-6	49.910805	27
>>END_MODULE