FastQCFastQC Report
Fri 17 Jun 2016
SRR1526445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63054
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG12902.0458654486630508No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5660.8976432898785169No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4750.7533225489263171No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.7469787800932535No Hit
GCTCAAGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC3910.6201034034319789No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.5312906397690869No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2910.46150918260538587No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA2570.40758714752434416No Hit
GCTCAAGGGGTTTTTTAATCACTGTGATATTTCCATTCCTTTTATCCTAAAACAAAGAGAAGAA2170.344149459193707No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2070.32829003711104765No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1540.24423510007295335No Hit
GCTCAAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC1530.2426491578646874No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1180.18714118057537982No Hit
GCTCAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1170.1855552383671139No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1140.18079741174231612No Hit
GCTCAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC1110.17603958511751833No Hit
GCTCAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1090.17286770070098645No Hit
GCTCAAGGGGTAGGCTTCTTGTATGTTCATGGGCATCTCTTTCTTTAGATTTGGGAAGTTTTCT1050.16652393186792272No Hit
GCTCAAGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1050.16652393186792272No Hit
GCTCAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.16335204745139087No Hit
GCTCAAGGGATGATTGGCATTATGCTCACCACTTTCTCCTACGTCAACAACATGGTGCGAGTGG900.14273479874393377No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC880.13956291432740192No Hit
GCTCAAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCAC860.13639102991087004No Hit
GCTCAAGGGGAAATGTGAAACTTAGGGGGTGGGGAGCCCGGAAAGCAGCCACGGGGAGCTGCTG830.13163320328607225No Hit
GCTCAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.1284613188695404No Hit
GCTCAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT800.12687537666127446No Hit
GCTCAAGGGGGCGGTGCAGATGCTCTGGGTAAACCTCATCATGGACACGCTGGCTTCCCTGGCT750.11894566561994481No Hit
GCTCAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA720.11418783899514702No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.11260189678688108No Hit
GCTCAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG690.10943001237034922No Hit
GCTCAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC690.10943001237034922No Hit
GCTCAAGGGGGGAAAAAAAGGAAAATGGAACATATACGATATTATCTTTCAACCATGAAATAAA680.1078440701620833No Hit
GCTCAAGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAGT660.10467218574555144No Hit
GCTCAAGGGATCGCAGCTATAACTCTTATGGGTCCTCAGTGGTGGTTGAAAACTGTCATTGAAC650.1030862435372855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAC201.9531009E-458.253453
ATAAATC258.355846E-658.253455
CCCATCG201.9531009E-458.253453
CGCTTTA201.9531009E-458.253458
GGAAGTT150.004586732558.25339554
ATGGTAG150.004586732558.25339555
TACCGCG150.004586732558.25339556
CTACGTA452.910383E-1158.20687539
CGTACTG452.910383E-1158.20687542
ACGTACT452.910383E-1158.20687541
TACGTAC452.910383E-1158.20687540
CCTCGTT201.9608426E-458.2068744
TCCTAAA201.9608426E-458.2068745
TGCGAAA201.9608426E-458.2068744
TGATCAG150.004601282558.20686745
TTAGATT150.004601282558.20686745
GATCAGC150.004601282558.20686746
TAGCCAG150.004601282558.20686747
TTATACT258.395591E-658.20686743
GGTGATC150.004601282558.20686743
Produced by FastQC (version 0.11.5)