FastQCFastQC Report
Fri 17 Jun 2016
SRR1526444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61096
Sequences flagged as poor quality0
Sequence length64
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG11151.824996726463271No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4910.8036532669896556No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4420.7234516171271441No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.6563441141809612No Hit
GCTCAAGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC3030.4959408144559382No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.41573916459342675No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2420.39609794421893413No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA2030.33226397800183316No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.30116537907555324No Hit
GCTCAAGGGGTTTTTTAATCACTGTGATATTTCCATTCCTTTTATCCTAAAACAAAGAGAAGAA1640.26843001178473225No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1300.21277988739033651No Hit
GCTCAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1150.18822836192222075No Hit
GCTCAAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC1130.18495482519313866No Hit
GCTCAAGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA1050.17186067827681026No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA870.14239884771507136No Hit
GCTCAAGGGGTAGGCTTCTTGTATGTTCATGGGCATCTCTTTCTTTAGATTTGGGAAGTTTTCT850.13912531098598926No Hit
GCTCAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC800.130941469163284No Hit
GCTCAAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCAC750.12275762734057875No Hit
GCTCAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG720.11784732224695563No Hit
GCTCAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT710.11621055388241455No Hit
GCTCAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG680.11130024878879141No Hit
GCTCAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA670.10966348042425035No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC660.1080267120597093No Hit
GCTCAAGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAGT640.10475317533062721No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10475317533062721No Hit
GCTCAAGGGATGATTGGCATTATGCTCACCACTTTCTCCTACGTCAACAACATGGTGCGAGTGG640.10475317533062721No Hit
GCTCAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG630.10311640696608615No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATT150.00455008258.36898837
GCTTGTC150.00456505658.3206338
GATATTA201.9414454E-458.3206338
CTTAAGT150.004595113558.22415547
GAAATAA150.004595113558.22415557
CATGCAT150.004595113558.22415558
GCAACTT150.004595113558.22415534
TAGAAAT150.004595113558.22415549
ATTCGTA150.004595113558.22415550
ATTAAGC150.004595113558.22415551
TTCGTAT150.004595113558.22415551
AAGCGTC150.004595113558.22415552
ATAGACT150.004595113558.22415548
AAGCGGC150.004595113558.22415534
TAAAACA150.004595113558.22415548
TTAAGTG150.004595113558.22415548
CCATACC150.004595113558.22415533
AAATTCG150.004595113558.22415548
GCGTCAG150.004595113558.22415554
AAAATTC150.004595113558.22415547
Produced by FastQC (version 0.11.5)