SRR1526443_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56205
Sequences flagged as poor quality	0
Sequence length	56
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	1201	2.1368205675651635	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	566	1.0070278444978205	No Hit
GCTCAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	442	0.7864069033004181	No Hit
GCTCAAGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	321	0.5711235655190819	No Hit
GCTCAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	291	0.5177475313584201	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	269	0.47860510630726805	No Hit
GCTCAAGGGGTTTTTTAATCACTGTGATATTTCCATTCCTTTTATCCTAAAACAAA	251	0.4465794858108709	No Hit
GCTCAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	249	0.44302108353349345	No Hit
GCTCAAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	211	0.3754114402633218	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	205	0.3647362334311894	No Hit
GCTCAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	200	0.35584022773774576	No Hit
GCTCAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	151	0.268659371941998	No Hit
GCTCAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	144	0.2562049639711769	No Hit
GCTCAAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	132	0.2348545503069122	No Hit
GCTCAAGGGAATCAAGATGAAAGCCAAGCATGGTGGCAAATTGTAGCACTTTTACA	112	0.19927052753313762	No Hit
GCTCAAGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	108	0.1921537229783827	No Hit
GCTCAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	107	0.19037452183969397	No Hit
GCTCAAGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT	98	0.1743617115914954	No Hit
GCTCAAGGGGTAGGCTTCTTGTATGTTCATGGGCATCTCTTTCTTTAGATTTGGGA	80	0.14233609109509832	No Hit
GCTCAAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.13877768881772085	No Hit
GCTCAAGGGGATTTAGATGCCAATCAGGAAGGCAAGTAGGATGGATGGATGAGTGA	78	0.13877768881772085	No Hit
GCTCAAGGGATGATTGGCATTATGCTCACCACTTTCTCCTACGTCAACAACATGGT	76	0.1352192865403434	No Hit
GCTCAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	75	0.13344008540165467	No Hit
GCTCAAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	73	0.1298816831242772	No Hit
GCTCAAGGGATGTATCCCACAGTAAGTTCACATGCAAGCTCCACACTGCAGAAGCG	72	0.12810248198558846	No Hit
GCTCAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	71	0.12632328084689975	No Hit
GCTCAAGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTAT	70	0.12454407970821102	No Hit
GCTCAAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70	0.12454407970821102	No Hit
GCTCAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.12276487856952228	No Hit
GCTCAAGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGC	68	0.12098567743083356	No Hit
GCTCAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	67	0.11920647629214483	No Hit
GCTCAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC	66	0.1174272751534561	No Hit
GCTCAAGGGATGACAACATCCCTTCTCCCACAGAGAAAGGACCACACTTCGTAACC	65	0.11564807401476737	No Hit
GCTCAAGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	64	0.11386887287607864	No Hit
GCTCAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	64	0.11386887287607864	No Hit
GCTCAAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC	63	0.11208967173738991	No Hit
GCTCAAGGGGGCGGTGCAGATGCTCTGGGTAAACCTCATCATGGACACGCTGGCTT	61	0.10853126946001246	No Hit
GCTCAAGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	61	0.10853126946001246	No Hit
GCTCAAGGGGGGAAAAAAAGGAAAATGGAACATATACGATATTATCTTTCAACCAT	60	0.10675206832132372	No Hit
GCTCAAGGGCCCGTGGTCTCCTGGGGACACTCAACAATAACCCCAGGGATGACTTC	60	0.10675206832132372	No Hit
GCTCAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	60	0.10675206832132372	No Hit
GCTCAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT	59	0.104972867182635	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGGG	15	0.007975016	50.64402	37
CGCCTCT	45	1.1641532E-10	50.64402	37
TTCTTAG	15	0.007975016	50.64402	37
TTTATCA	15	0.007975016	50.64402	37
TTCGCCT	45	1.1641532E-10	50.59819	35
AGGTGTA	15	0.0080036465	50.59819	36
TCGCCTC	45	1.1641532E-10	50.59819	36
CAGGTGT	15	0.0080036465	50.59819	35
TGGGCCA	20	3.9126666E-4	50.598186	36
AATAACC	20	3.9126666E-4	50.598186	36
GTGTAAG	15	0.008032354	50.55244	34
ATATGCT	15	0.008032354	50.55244	34
TGTGGGC	20	3.9302013E-4	50.55244	34
CTTCGCC	45	1.1823431E-10	50.55244	34
TCCGAAG	15	0.008032354	50.55244	34
ACAATAA	20	3.9302013E-4	50.55244	34
ACTTCGC	45	1.2187229E-10	50.41569	33
TTCCATT	45	1.2187229E-10	50.41569	31
ACAAATA	15	0.008118935	50.415688	33
CATGATT	15	0.008118935	50.415688	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content