FastQCFastQC Report
Fri 17 Jun 2016
SRR1526442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213115
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC35361.6591980855406707No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT29481.3832907115876405No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG24701.1589986626938509No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC10610.4978532717077634No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9740.4570302418881824No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8410.39462262158928274No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8200.384768786805246No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.3824226356661896No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC7130.3345611524294395No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG6140.2881073598761232No Hit
AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG5540.2599535462074467No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4930.23133050231095886No Hit
AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG4920.2308612720831476No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC4800.2252305093494123No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4780.22429204889378973No Hit
AACCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC4770.22382281866597845No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC3530.1656382704173803No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT3320.15578443563334352No Hit
AACCTAGGGGTGCTAACCTAGGGGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTC3150.1478075217605518No Hit
AACCTAGGGGATTTTGCTGATTGCACCAAGATTTCAGTTGAGACTTAGTTTTCAAAGATGCTGT2970.13936137765994885No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2950.13842291720432628No Hit
AACCTAGGGCGTTCAAACTACTTGCTGCTAACCTTTCCCGAGTGATTGCTTTTCATGCAAACTC2930.13748445674870374No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC2870.1346690753818361No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2760.12950754287591207No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGTGGCTAAGAGCACTGATTGTTCTTCCACAGGTTCT2250.10557680125753702No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT2220.10416911057410318No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCT2190.10276141989066935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAA201.9602594E-458.27483754
CAGTATA258.406396E-658.27483756
CGATGAT150.004599450658.26105550
TTGAGCG201.9671474E-458.23351747
CACGACT150.00460807158.23351347
GATGTAG150.00462534858.17851346
ATTGTAA201.9763614E-458.17851346
CAATTAT201.9786702E-458.1647844
CGACATT201.9786702E-458.1647842
TCTCATG150.00462967558.1647843
ATTATAC150.00462967558.1647842
CACAATT201.9786702E-458.1647842
TCAAGTA201.9786702E-458.1647844
TGCATCC150.004634004658.1510541
TCGACAT201.9809812E-458.1510541
GTACGTA453.092282E-1158.1510539
TACGTAG351.5996193E-858.1510540
CAACGTG150.00464701258.10990537
GCAACGT150.004651353758.09620336
CGGTATT150.004651353758.09620336
Produced by FastQC (version 0.11.5)