FastQCFastQC Report
Fri 17 Jun 2016
SRR1526441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205987
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT29641.4389257574507128No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC27441.3321229009597693No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG17970.8723851505192075No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC8740.4242986207867487No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8160.3961415040755No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG7860.38157747819037124No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.34905115371358386No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.3437110108890367No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6460.31361202405977073No Hit
AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG5220.2534140504012389No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC4800.23302441416205877No Hit
AACCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC4400.2136057129818872No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4010.19467247933121992No Hit
AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG3750.1820503235641084No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3680.1786520508575784No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC3110.1509804016758339No Hit
AACCTAGGGGTGCTAACCTAGGGGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTC2940.142727453674261No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT2790.13544544073169665No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.1252506226121066No Hit
AACCTAGGGGATTTTGCTGATTGCACCAAGATTTCAGTTGAGACTTAGTTTTCAAAGATGCTGT2570.1247651550826023No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC2510.12185234990557657No Hit
AACCTAGGGCGTTCAAACTACTTGCTGCTAACCTTTCCCGAGTGATTGCTTTTCATGCAAACTC2500.12136688237607228No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2330.11311393437449936No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC2090.10146271366639642No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT2060.10000631107788356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA150.004578901558.32621439
GCAACGT150.00458335458.311936
ACGCAAC150.0045878158.29759634
AAAGCGA201.9586862E-458.28330233
TAGAAAC150.00459226958.28329533
CTAAATC150.004623574758.18339558
GCAACTA150.0046280658.1691557
CCGAATG150.0046280658.1691556
TTATTAC150.0046280658.1691557
TCACAAT258.520989E-658.1406941
CACAATT201.9825596E-458.1406942
TGGATAG150.00464603358.1122550
TTGTAAC201.9897668E-458.09804547
CGTTGAA258.558147E-658.0980428
CGTAGAA150.00465503958.0838431
CGGGATA150.00469119357.97047824
ATCATCC150.00470480457.92808523
GATCATC150.00470934857.91396322
GATATCT150.00470934857.91396322
TACTGGT202.0285568E-457.8716515
Produced by FastQC (version 0.11.5)