##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526441_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 205987 Sequences flagged as poor quality 0 Sequence length 64 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.23790336283358 39.0 38.0 40.0 35.0 40.0 2 38.323340793351036 39.0 38.0 40.0 35.0 40.0 3 38.17086515168433 39.0 38.0 40.0 35.0 40.0 4 38.314383917431684 39.0 39.0 40.0 36.0 40.0 5 38.13141606023681 39.0 38.0 40.0 35.0 40.0 6 38.288537626160874 39.0 38.0 40.0 35.0 40.0 7 38.43460024176283 39.0 38.0 40.0 36.0 40.0 8 38.33011306538762 39.0 38.0 40.0 36.0 40.0 9 38.5210086073393 39.0 38.0 40.0 36.0 40.0 10 37.876453368416456 39.0 38.0 40.0 35.0 40.0 11 37.838698558646904 39.0 38.0 40.0 35.0 40.0 12 37.66689645463063 39.0 38.0 40.0 33.0 40.0 13 37.61654376247045 39.0 38.0 40.0 33.0 40.0 14 37.49224951089146 39.0 37.0 40.0 33.0 40.0 15 37.40565181297849 39.0 38.0 40.0 33.0 40.0 16 37.191992698568356 39.0 37.0 40.0 33.0 40.0 17 36.82996499779112 39.0 36.0 40.0 32.0 40.0 18 37.100083985882605 39.0 36.0 40.0 33.0 40.0 19 37.034846859267816 39.0 36.0 40.0 32.0 40.0 20 36.814735881390575 39.0 36.0 40.0 31.0 40.0 21 36.819983785384515 39.0 36.0 40.0 32.0 40.0 22 36.9095088525004 39.0 36.0 40.0 32.0 40.0 23 36.794346245151395 39.0 36.0 40.0 31.0 40.0 24 36.46491768898037 38.0 35.0 40.0 31.0 40.0 25 36.5231640831703 38.0 36.0 40.0 31.0 40.0 26 36.62212663905974 39.0 36.0 40.0 31.0 40.0 27 36.04326486622942 39.0 36.0 40.0 29.0 40.0 28 36.00672857995893 39.0 36.0 40.0 29.0 40.0 29 35.85741818658459 39.0 35.0 40.0 29.0 40.0 30 35.806050867287745 39.0 35.0 40.0 29.0 40.0 31 35.309636044993134 38.0 35.0 40.0 28.0 40.0 32 35.55674872686141 38.0 35.0 40.0 28.0 40.0 33 35.44139193250059 38.0 35.0 40.0 28.0 40.0 34 34.525610839519 38.0 33.0 40.0 24.0 40.0 35 34.76619883779073 38.0 34.0 40.0 26.0 40.0 36 33.68364508439853 38.0 33.0 40.0 21.0 40.0 37 33.06206216897183 38.0 32.0 39.0 17.0 40.0 38 33.00973847864186 37.0 32.0 39.0 18.0 40.0 39 32.44063945782986 37.0 31.0 39.0 12.0 40.0 40 32.70785535009491 37.0 32.0 39.0 15.0 40.0 41 31.822974265366263 37.0 30.0 39.0 4.0 40.0 42 31.424332603513815 37.0 30.0 39.0 4.0 40.0 43 31.465073038589814 36.0 30.0 39.0 4.0 40.0 44 31.313879031200997 36.0 30.0 39.0 4.0 40.0 45 31.172612834790545 36.0 29.0 39.0 4.0 40.0 46 30.843897915887897 36.0 30.0 39.0 4.0 40.0 47 30.430512605164402 36.0 28.0 39.0 4.0 40.0 48 30.343652754785495 36.0 28.0 39.0 4.0 40.0 49 30.249855573409974 36.0 28.0 39.0 4.0 40.0 50 29.62621427565817 35.0 27.0 39.0 4.0 40.0 51 29.32874404695442 35.0 27.0 39.0 4.0 40.0 52 29.27345900469447 35.0 27.0 39.0 4.0 40.0 53 28.851116818051626 35.0 25.0 38.0 4.0 40.0 54 28.477214581502714 35.0 23.0 38.0 4.0 40.0 55 28.442207517950163 35.0 24.0 38.0 4.0 40.0 56 27.446023292732065 34.0 17.0 38.0 4.0 40.0 57 27.1936141601169 34.0 16.0 38.0 4.0 40.0 58 26.937481491550436 33.0 14.0 38.0 4.0 40.0 59 26.619111885701525 33.0 8.0 38.0 4.0 40.0 60 26.282741143858594 33.0 4.0 38.0 4.0 40.0 61 26.037021753799998 33.0 4.0 38.0 4.0 39.0 62 25.736551335763906 33.0 4.0 37.0 4.0 39.0 63 25.222125668124686 33.0 4.0 37.0 4.0 39.0 64 25.277973852718862 33.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.048595254275937805 2 2 0.39108127387090263 2 3 0.03325583942892507 2 4 0.22652004650128532 2 5 0.2103878385978888 2 6 0.29989617369314203 2 7 -0.13688704432743037 2 8 0.2485193368344909 2 9 -0.21893428004408122 2 10 0.08927436130161226 2 11 0.11111449558897846 2 12 -0.146506591207185 2 13 0.1786051550110841 2 14 -0.23476597718318004 2 15 0.28731763253019693 2 16 -0.3595015134641599 2 17 0.33294330854602805 2 18 -0.3971634409511253 2 19 -0.007730306438382684 2 20 0.009653201961270952 2 21 0.366130057716191 2 22 0.05473269909980161 2 23 0.5979401251567964 2 24 -0.15265979549416642 2 25 0.40878645770304445 2 26 0.4091998109201356 2 27 0.8964728565166098 2 28 0.61509607256518 2 29 0.7619635380991667 2 30 0.759295336583925 2 31 1.2815460625696815 2 32 0.8020165068038949 2 33 0.8163686897960858 2 34 1.012432809029292 2 35 1.2344010706699606 2 36 0.3049618914218044 2 37 -0.08754449920198937 2 38 -0.06365633700134765 2 39 -3.5421283015319744 2 40 -0.38744571476459555 2 41 -1.6410201879192208 2 42 -1.445107669624452 2 43 -2.003767890536576 2 44 -1.0691388196514318 2 45 -1.5998004014695795 2 46 -1.0094758762040676 2 47 -1.1887629523208005 2 48 -0.784316783689281 2 49 -1.01935449267852 2 50 -1.2574500010630736 2 51 -1.4932147317287416 2 52 -1.6639527793058875 2 53 -1.7993518782498157 2 54 -1.3733077507218745 2 55 -1.502519126214704 2 56 -1.0346034319262323 2 57 -0.8355703055009656 2 58 -0.7169718314141491 2 59 -0.8701162973792194 2 60 -0.958578958180361 2 61 -1.1076037745990455 2 62 -1.025617207497035 2 63 -0.9954515587157715 2 64 -1.1756237944698356 3 1 -0.17709046599207312 3 2 0.47666544472517103 3 3 0.013155336916362614 3 4 0.3511274585615851 3 5 -0.07669863543896582 3 6 0.19735220384389862 3 7 0.19950847493555557 3 8 0.4528744457122116 3 9 0.27342334139645175 3 10 -0.11133807588431921 3 11 -0.23588089804586332 3 12 -0.3089071355957955 3 13 -0.34023906609444055 3 14 -0.4391734311195279 3 15 0.07309548010138656 3 16 0.24490070596632307 3 17 0.010864162817384226 3 18 -0.08521306406669993 3 19 0.08198912404737513 3 20 0.011145036132127473 3 21 0.0925747813342781 3 22 0.2825122217128637 3 23 0.3437263479373698 3 24 0.1685556567671398 3 25 0.20513800711677987 3 26 0.023992524487972844 3 27 0.8153380156455867 3 28 0.8728693137712114 3 29 0.7541379518345153 3 30 0.6005327737698565 3 31 1.4505159118159128 3 32 1.0441909205392435 3 33 0.7508326763957527 3 34 1.3695307989790422 3 35 0.7620392616247358 3 36 -4.341813652966806 3 37 -4.000285286471669 3 38 -3.6879496787098915 3 39 -19.60470681911991 3 40 -6.227479215602113 3 41 -4.3686688478857185 3 42 -4.119652267614402 3 43 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19 0.23539575109122524 4 20 -0.1901405797646163 4 21 0.04474680898522365 4 22 -0.10651412831136753 4 23 0.15200632651043833 4 24 0.1900591131352769 4 25 0.03487042555414632 4 26 0.7570741764023694 4 27 1.017771502878709 4 28 1.1868758949002043 4 29 1.3698051201634698 4 30 1.0167509203402716 4 31 0.04740900673211712 4 32 -0.4213601852434792 4 33 -0.02352132712438504 4 34 0.3940952455775175 4 35 -4.6082385232386684 4 36 -0.8056557058709188 4 37 -0.34510583591941213 4 38 -0.7410833674582022 4 39 -0.3117190375725869 4 40 -0.3155337012282118 4 41 0.4880513010824714 4 42 0.8368561628628548 4 43 0.8598107896664722 4 44 0.9677689637665026 4 45 0.9604523481074061 4 46 1.0560645214270679 4 47 1.2531786720446831 4 48 1.0293464988826315 4 49 1.177742588539754 4 50 1.2946333322702586 4 51 0.9420908943287891 4 52 0.785338675854856 4 53 0.6398930456080514 4 54 0.5204105233897991 4 55 0.6533871766617771 4 56 0.31673434303146664 4 57 0.10592186877991594 4 58 0.1574200890596238 4 59 0.44322972630944335 4 60 0.5122861010412976 4 61 0.26551911034173514 4 62 0.2722360073929835 4 63 0.5078532213180686 4 64 0.21397011415960776 5 1 -0.0816130790573979 5 2 0.032511686242038706 5 3 0.10026614870727713 5 4 0.1938203042332418 5 5 0.0901666186666148 5 6 -0.17271980568830259 5 7 -0.10751633986007647 5 8 0.26054682824342024 5 9 -0.09157173708875632 5 10 0.04078853655934722 5 11 -2.1813038892907457 5 12 -2.414279357530212 5 13 -2.416755669731181 5 14 -2.0052771455680656 5 15 -1.8586767832773674 5 16 -19.404013970508835 5 17 -2.792378856402422 5 18 -3.105443080126385 5 19 -3.033718278946978 5 20 -2.9663455093789324 5 21 -2.9541019010466982 5 22 -2.9447840534775196 5 23 -2.5605113353243 5 24 -2.8928337645663333 5 25 -2.9307217038089775 5 26 -0.07630276639945066 5 27 0.4684230283379165 5 28 0.7421901447301309 5 29 0.5645301188551812 5 30 -0.1598412613542166 5 31 -0.5547941436158865 5 32 -0.4443859846050344 5 33 -0.7742670490355295 5 34 0.011788479132384566 5 35 -4.5336401664434405 5 36 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0.030516535760131802 118 59 0.43815358417746353 118 60 0.5376668117011931 118 61 0.026940430138687077 118 62 -0.11355079463747231 118 63 0.3606450994906574 118 64 0.16828483497178937 119 1 0.050366616374077466 119 2 0.495618075901362 119 3 0.007875128769029516 119 4 0.36936585868402716 119 5 0.35857417531531155 119 6 0.3723898285154803 119 7 0.03694438545260681 119 8 0.35173422685140565 119 9 0.17330345261242996 119 10 0.29985494505795884 119 11 0.13485603535208668 119 12 -0.011327868702956323 119 13 -0.028247636867085646 119 14 0.06372175547333114 119 15 0.08271209107503097 119 16 0.27456680294869784 119 17 0.3137491461094797 119 18 -0.034298593235391195 119 19 -0.16054333520319375 119 20 0.17534165373792376 119 21 -0.1582988762939621 119 22 -0.3603212349103515 119 23 -2.7793744895632244E-4 119 24 -0.601819059453554 119 25 -0.6199519094712329 119 26 0.005805140869377112 119 27 0.38325373638124205 119 28 0.4000738644433497 119 29 0.48148024706701875 119 30 0.2959387375991511 119 31 0.5702516463260281 119 32 0.36036570308139204 119 33 0.5653111601852601 119 34 0.8407957531917276 119 35 0.2953147762537185 119 36 0.6715016545351702 119 37 0.42646776814149945 119 38 0.6294750081763141 119 39 0.7238139573512754 119 40 0.6743140145078286 119 41 0.401756884838818 119 42 0.6236581933197094 119 43 0.7329072363669802 119 44 0.4347740399086355 119 45 0.7269498100363414 119 46 0.22357721178239842 119 47 0.5323664893035662 119 48 0.7653871080196843 119 49 0.19297101493569357 119 50 0.9139226723717826 119 51 0.8913294730089909 119 52 0.6685874068193236 119 53 0.9850707105826721 119 54 0.6219333660293955 119 55 1.1305445370678662 119 56 0.21013535825989038 119 57 0.44602339162255333 119 58 0.548283033222063 119 59 1.0016053608272095 119 60 0.8371591974879955 119 61 0.9406460138950337 119 62 1.2214745860731853 119 63 0.680442053805379 119 64 1.122599555783971 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 154.0 5 21.0 6 50.0 7 71.0 8 187.0 9 159.0 10 253.0 11 345.0 12 401.0 13 617.0 14 745.0 15 902.0 16 1204.0 17 1331.0 18 1418.0 19 1706.0 20 2141.0 21 2747.0 22 3652.0 23 3642.0 24 3873.0 25 4839.0 26 3055.0 27 3578.0 28 4175.0 29 4860.0 30 6073.0 31 6696.0 32 7822.0 33 9590.0 34 11934.0 35 14885.0 36 19303.0 37 24994.0 38 30926.0 39 27569.0 40 69.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 99.2999456268448 0.5349930091657604 0.06602454559577443 0.09903681839366166 8 99.41662214018555 0.31087579540486715 0.18846845096420073 0.08403361344537816 9 99.05964939535019 0.5510056459873681 0.14466932379227815 0.2446756348701617 10 64.67689708573842 21.890701840407402 4.879919606577114 8.55248146727706 11 41.668648992412145 22.865035172122514 15.787889527008986 19.67842630845636 12 41.78419026443416 14.049430303854127 21.04356100142242 23.12281843028929 13 33.732711287605525 13.387738061139782 26.259424138416502 26.620126512838187 14 25.182657157975992 17.02874453242195 36.411521115410196 21.377077194191866 15 26.96097485132351 17.23432580557391 34.774750262370276 21.029949080732305 16 35.22690854706665 19.945907100057298 23.001621815851063 21.825562537024986 17 37.8674004141914 18.31581608346054 20.60748072454327 23.209302777804787 18 35.609528756669114 19.200240791894636 23.2485545204309 21.941675931005356 19 34.96531334501692 22.343643045434906 21.37756266172137 21.313480947826804 20 29.02028962985266 29.705028215114755 19.757379150924155 21.51730300410843 21 25.36325107895158 21.273672610407456 32.63701107351435 20.726065237126615 22 27.271623937432942 18.940030196080336 31.906867909139898 21.881477957346824 23 30.002524443408774 19.701824395832727 29.276261493499554 21.019389667258938 24 27.834500973438008 26.524607833141562 21.715678420748553 23.92521277267188 25 34.21762000281702 19.291761174602094 20.54679417750169 25.943824645079193 26 23.74402175232454 17.40914277390692 24.45194338569105 34.3948920880775 27 21.515145619735023 19.011789653897786 29.526176533530474 29.946888192836713 28 21.89981183723678 21.98976044497819 22.599952351548332 33.5104753662367 29 29.003422060624917 25.702397386790064 22.761077949096848 22.53310260348817 30 30.797027439765774 19.53320299201416 26.078477146497807 23.591292421722258 31 27.799319843729474 18.07960455578206 31.141718683863562 22.97935691662491 32 37.229113080854006 16.881658214492358 26.442744825373055 19.446483879280585 33 35.8345454280113 17.513511409905284 26.61857204706981 20.033371115013594 34 23.922799187257286 20.449639674318206 37.68972523644089 17.937835901983618 35 21.911268777646388 32.283682973474065 28.9293760979479 16.875672150931646 36 30.492996162013675 26.69640943716028 25.075006331703324 17.735588069122716 37 28.37547553057278 28.636560690122113 25.280447351885314 17.707516427419787 38 30.648452528794408 25.532422626439722 25.429663720262013 18.38946112450386 39 26.52228714361089 19.808785883180583 27.333261680964572 26.33566529224396 40 29.002479552219175 19.176340492695306 28.376502223802724 23.44467773128279 41 21.160416209761923 21.151156273606745 29.48022516265809 28.208202353973245 42 22.665599828265037 19.544609620085186 32.98335829670142 24.80643225494836 43 20.829166199134537 18.255706737829122 35.5269127755797 25.38821428745664 44 27.643981099966354 17.667337952691405 30.294179316263488 24.394501631078754 45 24.272417729299395 19.0869001043933 27.17347824814385 29.46720391816346 46 21.511032174328847 22.829428239633856 27.155445824753837 28.50409376128346 47 19.91393106678637 23.054178539365314 34.06944064951794 22.962449744330378 48 24.47523110319764 26.12600307324569 29.392424205468426 20.006341618088246 49 31.330174731510713 19.69084086794865 27.309544605085318 21.66943979545532 50 21.1456821967865 22.566304671737527 27.881425898514404 28.406587232961567 51 21.7189048290809 27.173281656521674 29.3298448231257 21.777968691271727 52 22.935493162656044 26.806489252115156 28.49568668805015 21.76233089717865 53 25.939346583972263 19.169800263710503 30.630463446793964 24.26038970552327 54 20.80975376337261 22.34000478508616 28.255933750970446 28.59430770057079 55 29.1380118787121 19.737124882775866 26.228899656142545 24.89596358236949 56 20.870274574168242 23.71078122481279 25.144467728620484 30.274476472398483 57 24.09822771948336 28.288098169099403 24.253082439377064 23.360591672040172 58 24.3976345242168 31.10014173305313 23.69385660524901 20.808367137481063 59 29.66044117718939 25.521411219567035 23.42452429016223 21.39362331308135 60 31.781865477517652 23.973674431340335 23.55268047487727 20.691779616264743 61 27.213925244146107 20.541982383374126 23.999355131831575 28.24473724064819 62 23.270904284688186 20.400275580355615 25.507795894634487 30.821024240321705 63 22.432066611613056 21.500715859838067 24.592839447053247 31.474378081495637 64 24.15455865883538 20.260271225896055 27.013531951936386 28.571638163332175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 2.5 3 5.0 4 4.0 5 3.5 6 4.0 7 40.5 8 77.0 9 112.5 10 161.0 11 174.0 12 234.5 13 412.0 14 529.0 15 717.5 16 956.5 17 1007.0 18 1137.0 19 1267.0 20 1495.0 21 2099.5 22 2476.0 23 2684.5 24 3062.0 25 3231.0 26 3593.5 27 3332.0 28 2708.0 29 2837.5 30 2665.5 31 2364.0 32 2318.5 33 2273.0 34 2301.5 35 2367.0 36 2404.0 37 2528.0 38 3024.0 39 3396.0 40 3241.0 41 3185.5 42 3285.0 43 3582.5 44 3880.0 45 4574.5 46 5008.0 47 4747.0 48 4884.5 49 5175.0 50 5328.0 51 5729.0 52 7305.5 53 8481.0 54 8949.5 55 10006.5 56 10595.0 57 12166.5 58 13738.0 59 15381.5 60 18016.5 61 19008.0 62 15750.0 63 11706.0 64 10920.0 65 10458.0 66 7755.0 67 5514.0 68 4579.5 69 3645.0 70 3248.5 71 2509.5 72 2167.0 73 1746.5 74 1141.0 75 956.0 76 767.5 77 457.5 78 336.0 79 267.5 80 153.5 81 108.0 82 76.0 83 44.0 84 35.5 85 19.0 86 11.0 87 9.0 88 6.5 89 6.0 90 3.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0014564025885128672 8 0.05679970095200182 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08301494754523343 16 0.02184603882769301 17 0.1383582459087224 18 0.0 19 0.0 20 0.03349725953579595 21 0.0 22 0.0 23 4.854675295042891E-4 24 0.007767480472068626 25 0.04660488283241175 26 0.015534960944137251 27 0.1859340638001427 28 0.15243680426434678 29 0.12767796025962805 30 0.18107938850509983 31 0.21651851815891296 32 0.15534960944137252 33 0.20389636239180145 34 0.36701345230524257 35 0.23787908945710168 36 0.325748712297378 37 0.3349725953579595 38 0.3165248292367965 39 0.6286804507080545 40 0.3437110108890367 41 0.38934495866243984 42 0.4951768800943748 43 0.490322204799332 44 0.4408045167898945 45 0.4820692567977591 46 0.505371698213965 47 0.5024588930369392 48 0.48158378926825474 49 0.5068281008024778 50 0.6252821780015244 51 0.545665503162821 52 0.5612004641069582 53 0.5908139834067199 54 0.5747935549330783 55 0.6073198794098656 56 0.6165437624704472 57 0.6209129702359858 58 0.6684887881274061 59 0.6786836062469962 60 0.7141227359008092 61 0.62819498317855 62 0.6447008791816959 63 0.6495555544767389 64 0.732085034492468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 205987.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.218936146455846 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42235176723912 36.245976687849236 2 8.251667454440419 7.62766582357139 3 3.9945381019904413 5.538699044114435 4 2.376975999159708 4.394452077072826 5 1.6259650228454388 3.7575186783631978 6 1.1228401869649705 3.11378873424051 7 0.7846226563730897 2.5385097117779276 8 0.5798014810146526 2.143824610290941 9 0.44955622078672336 1.8700209236505216 >10 2.190011028832519 17.527319685222853 >50 0.12919489522609107 4.053653871360814 >100 0.06197153510845018 5.083330501439411 >500 0.007352555012866971 2.46180584211625 >1k 0.003151095005514416 3.64343380892969 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 2964 1.4389257574507128 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 2744 1.3321229009597693 No Hit AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG 1797 0.8723851505192075 No Hit AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 874 0.4242986207867487 No Hit AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 816 0.3961415040755 No Hit AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG 786 0.38157747819037124 No Hit AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 719 0.34905115371358386 No Hit AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.3437110108890367 No Hit AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 646 0.31361202405977073 No Hit AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG 522 0.2534140504012389 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC 480 0.23302441416205877 No Hit AACCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC 440 0.2136057129818872 No Hit AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 401 0.19467247933121992 No Hit AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG 375 0.1820503235641084 No Hit AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 368 0.1786520508575784 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC 311 0.1509804016758339 No Hit AACCTAGGGGTGCTAACCTAGGGGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGTGAGTTTTC 294 0.142727453674261 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT 279 0.13544544073169665 No Hit AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.1252506226121066 No Hit AACCTAGGGGATTTTGCTGATTGCACCAAGATTTCAGTTGAGACTTAGTTTTCAAAGATGCTGT 257 0.1247651550826023 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC 251 0.12185234990557657 No Hit AACCTAGGGCGTTCAAACTACTTGCTGCTAACCTTTCCCGAGTGATTGCTTTTCATGCAAACTC 250 0.12136688237607228 No Hit AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 233 0.11311393437449936 No Hit AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC 209 0.10146271366639642 No Hit AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT 206 0.10000631107788356 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.004369207765538602 0.0 0.0 0.0 0.0 7 0.004854675295042891 0.0 0.0 0.0 0.0 8 0.004854675295042891 0.0 0.0 0.0 0.0 9 0.004854675295042891 0.0 0.0 0.0 0.0 10 0.009709350590085783 0.0 0.0 0.0 0.0 11 0.015534960944137251 0.0 0.0 0.0 0.0 12 0.01650589600314583 0.0 0.0 0.0 0.0 13 0.016991363532650117 0.0 0.0 0.0 0.0 14 0.022816973886701588 0.0 0.0 0.0 0.0 15 0.024273376475214454 0.0 0.0 0.0 0.0 16 0.0267007141227359 0.0 0.0 0.0 0.0 17 0.02864258424075306 0.0 0.0 0.0 0.0 18 0.030584454358770212 0.0 0.0 0.0 0.0 19 0.03204085694728308 0.0 0.0 0.0 0.0 20 0.03543912965381311 0.0 0.0 0.0 0.0 21 0.03592459718331739 0.0 0.0 0.0 0.0 22 0.04175020753736886 0.0 0.0 0.0 0.0 23 0.042721142596377444 0.0 0.0 0.0 0.0 24 0.045148480243898885 0.0 0.0 0.0 0.0 25 0.045148480243898885 0.0 0.0 0.0 0.0 26 0.04660488283241175 0.0 0.0 0.0 0.0 27 0.04660488283241175 0.0 0.0 0.0 0.0 28 0.05485783083398467 0.0 0.0 0.0 0.0 29 0.05728516848150612 0.0 0.0 0.0 0.0 30 0.061654376247044715 0.0 0.0 0.0 0.0 31 0.06359624636506188 0.0 0.0 0.0 0.0 32 0.06456718142407045 0.0 0.0 0.0 0.0 33 0.06456718142407045 0.0 0.0 0.0 0.0 34 0.06796545413060047 0.0 0.0 0.0 0.0 35 0.0849568176632506 0.0 0.0 0.0 0.0 36 0.08835509036978062 0.0 0.0 0.0 0.0 37 0.09029696048779777 0.0 0.0 0.0 0.0 38 0.09223883060581493 0.0 0.0 0.0 0.0 39 0.09418070072383208 0.0 0.0 0.0 0.0 40 0.10000631107788356 0.0 0.0 0.0 0.0 41 0.10291911625490929 0.0 0.0 0.0 0.0 42 0.10825925907945647 0.0 0.0 0.0 0.0 43 0.11020112919747363 0.0 0.0 0.0 0.0 44 0.11311393437449936 0.0 0.0 0.0 0.0 45 0.1160267395515251 0.0 0.0 0.0 0.0 46 0.1203959473170637 0.0 0.0 0.0 0.0 47 0.12379422002359372 0.0 0.0 0.0 0.0 48 0.13010529790714948 0.0 0.0 0.0 0.0 49 0.1335035706136795 0.0 0.0 0.0 0.0 50 0.1378727783792181 0.0 0.0 0.0 0.0 51 0.13981464849723527 0.0 0.0 0.0 0.0 52 0.14272745367426098 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGTA 15 0.0045789015 58.326214 39 GCAACGT 15 0.004583354 58.3119 36 ACGCAAC 15 0.00458781 58.297596 34 AAAGCGA 20 1.9586862E-4 58.283302 33 TAGAAAC 15 0.004592269 58.283295 33 CTAAATC 15 0.0046235747 58.183395 58 GCAACTA 15 0.00462806 58.16915 57 CCGAATG 15 0.00462806 58.16915 56 TTATTAC 15 0.00462806 58.16915 57 TCACAAT 25 8.520989E-6 58.14069 41 CACAATT 20 1.9825596E-4 58.14069 42 TGGATAG 15 0.004646033 58.11225 50 TTGTAAC 20 1.9897668E-4 58.098045 47 CGTTGAA 25 8.558147E-6 58.09804 28 CGTAGAA 15 0.004655039 58.08384 31 CGGGATA 15 0.004691193 57.970478 24 ATCATCC 15 0.004704804 57.928085 23 GATCATC 15 0.004709348 57.913963 22 GATATCT 15 0.004709348 57.913963 22 TACTGGT 20 2.0285568E-4 57.87165 15 >>END_MODULE