FastQCFastQC Report
Fri 17 Jun 2016
SRR1526440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187786
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC31921.6998072273758429No Hit
AACCTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA26081.3888149276303878No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC16000.8520336979327532No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC12150.6470130893676845No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC11760.6262447679805736No Hit
AACCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA9620.5122852608820678No Hit
AACCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC9300.4952445869234128No Hit
AACCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9260.4931145026785809No Hit
AACCTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG8290.4414599597414077No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC6860.36530944798866793No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT4630.24655725133929046No Hit
AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA4530.24123204072721074No Hit
AACCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4050.21567102978922817No Hit
AACCTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA3260.17360186595379848No Hit
AACCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3120.16614657109688688No Hit
AACCTAGGGGTGCTAACCTAGGGGGCTTTGGCCCTAAGCCATTGTCTCTCTGGAGT3080.164016486852055No Hit
AACCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2880.1533660656278956No Hit
AACCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC2740.145910770770984No Hit
AACCTAGGGCGTTCAAACTACTTGCTGCTAACCTTTCCCGAGTGATTGCTTTTCAT2570.13685791273044848No Hit
AACCTAGGGGATTTTGCTGATTGCACCAAGATTTCAGTTGAGACTTAGTTTTCAAA2540.1352603495468246No Hit
AACCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTT2330.12407740726145719No Hit
AACCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2310.12301236513904126No Hit
AACCTAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCG2220.11821967558816951No Hit
AACCTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCA2220.11821967558816951No Hit
AACCTAGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCC2170.11555707028212966No Hit
AACCTAGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGAATGCTCTTCCAGA2000.10650421224159415No Hit
AACCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC1890.10064648056830648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAC402.386514E-950.62185336
CAGTACG550.050.62185337
TCGTCAA150.00802699550.6081334
AACTCTA402.391971E-950.6081335
CGTCAAA150.00802699550.6081335
ATTTCTC150.00802699550.6081335
CTCGTAG251.9370167E-550.60812834
GATAAGA150.00812203250.45771832
CCTAAGC203.9911873E-450.45771833
GTCGTCA150.00812203250.45771833
ACGTAAG203.9911873E-450.45771833
CACGTAA203.9911873E-450.45771832
TGCGTCA309.813721E-750.44408831
AGCGTAT251.974604E-550.44408431
AACTTTC150.008270550550.22700540
TACGTAG550.050.22700540
CGACGCT150.0082793550.213539
GTAGCGG150.0082793550.213538
AGTACGT600.050.213538
GTACGTA600.050.213539
Produced by FastQC (version 0.11.5)